Restriction map for
pFN21AE1295
(Linear) (Six-base) MAPSORT of: KIEE1295.seq Check: 8273 from: 1 to: 1512
KIEE1295.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 680 1512
Size: 596 84 832
Fragments arranged by size:
832 596 84
AflIII A'CryG_T
Cuts at: 0 517 1512
Size: 517 995
AgeI A'CCGG_T
Cuts at: 0 1376 1512
Size: 1376 136
ApaLI G'TGCA_C
Cuts at: 0 277 1512
Size: 277 1235
ApoI r'AATT_y
Cuts at: 0 231 370 378 498 650 902 1070 1322
Size: 231 139 8 120 152 252 168 252
Cuts at: 1322 1406 1512
Size: 84 106
Fragments arranged by size:
252 252 231 168 152 139 120 106
84 8
BanII G_rGCy'C
Cuts at: 0 89 1512
Size: 89 1423
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 1512
Size: 18 1494
BclI T'GATC_A
Cuts at: 0 59 1512
Size: 59 1453
BglI GCCn_nnn'nGGC
Cuts at: 0 1207 1512
Size: 1207 305
Bme1580I G_kGCm'C
Cuts at: 0 281 1512
Size: 281 1231
BmrI ACTGGGnnnn_n'
Cuts at: 0 121 1512
Size: 121 1391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 848 932 1016 1184 1268 1352 1436
Size: 91 757 84 84 168 84 84 84
Cuts at: 1436 1512
Size: 76
Fragments arranged by size:
757 168 91 84 84 84 84 84
76
BsaWI w'CCGG_w
Cuts at: 0 1376 1512
Size: 1376 136
BsiHKAI G_wGCw'C
Cuts at: 0 281 1512
Size: 281 1231
BsmI GAATG_Cn'
Cuts at: 0 1210 1512
Size: 1210 302
Bsp1286I G_dGCh'C
Cuts at: 0 89 281 1512
Size: 89 192 1231
Fragments arranged by size:
1231 192 89
BsrDI GCAATG_nn'
Cuts at: 0 855 1275 1512
Size: 855 420 237
Fragments arranged by size:
855 420 237
BsrFI r'CCGG_y
Cuts at: 0 1376 1512
Size: 1376 136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 276 1512
Size: 276 1236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 124 1512
Size: 124 1388
BtsI GCAGTG_nn'
Cuts at: 0 1203 1512
Size: 1203 309
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 837 872 1257 1292 1512
Size: 837 35 385 35 220
Fragments arranged by size:
837 385 220 35 35
DraI TTT'AAA
Cuts at: 0 1038 1512
Size: 1038 474
EaeI y'GGCC_r
Cuts at: 0 160 1512
Size: 160 1352
EarI CTCTTCn'nnn_
Cuts at: 0 94 656 1512
Size: 94 562 856
Fragments arranged by size:
856 562 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 596 680 848 932 1016 1184 1268
Size: 91 505 84 168 84 84 168 84
Cuts at: 1268 1352 1436 1512
Size: 84 84 76
Fragments arranged by size:
505 168 168 91 84 84 84 84
84 84 76
EcoRI G'AATT_C
Cuts at: 0 902 1322 1512
Size: 902 420 190
Fragments arranged by size:
902 420 190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 189 1512
Size: 189 1323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 51 83 555 587 1512
Size: 51 32 472 32 925
Fragments arranged by size:
925 472 51 32 32
HindIII A'AGCT_T
Cuts at: 0 947 1367 1512
Size: 947 420 145
Fragments arranged by size:
947 420 145
MfeI C'AATT_G
Cuts at: 0 759 1512
Size: 759 753
MscI TGG'CCA
Cuts at: 0 162 1512
Size: 162 1350
MslI CAynn'nnrTG
Cuts at: 0 1095 1499 1512
Size: 1095 404 13
Fragments arranged by size:
1095 404 13
NdeI CA'TA_TG
Cuts at: 0 1496 1512
Size: 1496 16
NspI r_CATG'y
Cuts at: 0 109 521 1512
Size: 109 412 991
Fragments arranged by size:
991 412 109
PciI A'CATG_T
Cuts at: 0 517 1512
Size: 517 995
PsiI TTA'TAA
Cuts at: 0 293 1512
Size: 293 1219
SexAI A'CCwGG_T
Cuts at: 0 20 1512
Size: 20 1492
SfcI C'TryA_G
Cuts at: 0 12 1512
Size: 12 1500
SspI AAT'ATT
Cuts at: 0 335 1512
Size: 335 1177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1489 1512
Size: 1489 23
XbaI T'CTAG_A
Cuts at: 0 1477 1512
Size: 1477 35
XmnI GAAnn'nnTTC
Cuts at: 0 418 1512
Size: 418 1094
Enzymes that do cut:
AcuI AflIII AgeI ApaLI ApoI BanII BbsI BclI
BglI Bme1580I BmrI BpmI BsaWI BsiHKAI BsmI Bsp1286I
BsrDI BsrFI BstAPI BtgZI BtsI CspCI DraI EaeI
EarI Eco57MI EcoRI FalI Hin4I HindIII MfeI MscI
MslI NdeI NspI PciI PsiI SexAI SfcI SspI
TaqII XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI ClaI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MluI MmeI MspA1I NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI SwaI TatI TstI Tth111I XcmI XhoI XmaI
ZraI