Restriction map for
pFN21AE1282
(Linear) (Six-base) MAPSORT of: KIEE1282.seq Check: 6920 from: 1 to: 2382
KIEE1282.orf
With 182 enzymes: SgfI *
July 3, 2012 16:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3 2382
Size: 3 2379
Acc65I G'GTAC_C
Cuts at: 0 895 2382
Size: 895 1487
AclI AA'CG_TT
Cuts at: 0 2154 2382
Size: 2154 228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 635 1687 1744 2382
Size: 190 445 1052 57 638
Fragments arranged by size:
1052 638 445 190 57
AfeI AGC'GCT
Cuts at: 0 436 450 795 2382
Size: 436 14 345 1587
Fragments arranged by size:
1587 436 345 14
AflIII A'CryG_T
Cuts at: 0 1280 2382
Size: 1280 1102
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1945 2382
Size: 1945 437
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1840 1872 2382
Size: 1840 32 510
Fragments arranged by size:
1840 510 32
AlwNI CAG_nnn'CTG
Cuts at: 0 774 2382
Size: 774 1608
ApaI G_GGCC'C
Cuts at: 0 580 845 880 2382
Size: 580 265 35 1502
Fragments arranged by size:
1502 580 265 35
ApaLI G'TGCA_C
Cuts at: 0 1930 2083 2382
Size: 1930 153 299
Fragments arranged by size:
1930 299 153
ApoI r'AATT_y
Cuts at: 0 142 2031 2203 2382
Size: 142 1889 172 179
Fragments arranged by size:
1889 179 172 142
AvaI C'yCGr_G
Cuts at: 0 208 717 889 900 1272 1356 1557 2067
Size: 208 509 172 11 372 84 201 510
Cuts at: 2067 2382
Size: 315
Fragments arranged by size:
510 509 372 315 208 201 172 84
11
BanI G'GyrC_C
Cuts at: 0 895 1406 1465 1755 2043 2382
Size: 895 511 59 290 288 339
Fragments arranged by size:
895 511 339 290 288 59
BanII G_rGCy'C
Cuts at: 0 580 640 845 870 880 1273 1643 2382
Size: 580 60 205 25 10 393 370 739
Fragments arranged by size:
739 580 393 370 205 60 25 10
BbeI G_GCGC'C
Cuts at: 0 1410 1469 1759 2047 2382
Size: 1410 59 290 288 335
Fragments arranged by size:
1410 335 290 288 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 926 2382
Size: 926 1456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 126 160 486 520 2382
Size: 126 34 326 34 1862
Fragments arranged by size:
1862 326 126 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2157 2382
Size: 2157 225
BglI GCCn_nnn'nGGC
Cuts at: 0 250 575 584 2245 2382
Size: 250 325 9 1661 137
Fragments arranged by size:
1661 325 250 137 9
BlpI GC'TnA_GC
Cuts at: 0 1063 2382
Size: 1063 1319
Bme1580I G_kGCm'C
Cuts at: 0 580 752 845 880 1463 1766 1900 1934
Size: 580 172 93 35 583 303 134 34
Cuts at: 1934 2087 2185 2382
Size: 153 98 197
Fragments arranged by size:
583 580 303 197 172 153 134 98
93 35 34
BmgBI CAC'GTC
Cuts at: 0 917 2382
Size: 917 1465
BmrI ACTGGGnnnn_n'
Cuts at: 0 1966 2382
Size: 1966 416
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1441 2382
Size: 1441 941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 1272 1393 2382
Size: 451 821 121 989
Fragments arranged by size:
989 821 451 121
Bpu10I CC'TnA_GC
Cuts at: 0 254 445 2382
Size: 254 191 1937
Fragments arranged by size:
1937 254 191
BsaI GGTCTCn'nnnn_
Cuts at: 0 1412 2369 2382
Size: 1412 957 13
Fragments arranged by size:
1412 957 13
BsaAI yAC'GTr
Cuts at: 0 2017 2382
Size: 2017 365
BsaHI Gr'CG_yC
Cuts at: 0 661 670 850 1211 1407 1466 1496 1756
Size: 661 9 180 361 196 59 30 260
Cuts at: 1756 2044 2382
Size: 288 338
Fragments arranged by size:
661 361 338 288 260 196 180 59
30 9
BsaWI w'CCGG_w
Cuts at: 0 1635 1817 2100 2382
Size: 1635 182 283 282
Fragments arranged by size:
1635 283 282 182
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 840 870 1006 1036 1201 1231
Size: 187 30 623 30 136 30 165 30
Cuts at: 1231 1270 1300 2382
Size: 39 30 1082
Fragments arranged by size:
1082 623 187 165 136 39 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 622 1105 2382
Size: 622 483 1277
Fragments arranged by size:
1277 622 483
BseYI C'CCAG_C
Cuts at: 0 737 1188 1481 1546 1671 2194 2269 2347
Size: 737 451 293 65 125 523 75 78
Cuts at: 2347 2382
Size: 35
Fragments arranged by size:
737 523 451 293 125 78 75 65
35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1131 2121 2382
Size: 1131 990 261
Fragments arranged by size:
1131 990 261
BsiEI CG_ry'CG
Cuts at: 0 201 290 1344 2382
Size: 201 89 1054 1038
Fragments arranged by size:
1054 1038 201 89
BsiHKAI G_wGCw'C
Cuts at: 0 1006 1934 2087 2382
Size: 1006 928 153 295
Fragments arranged by size:
1006 928 295 153
BsmBI CGTCTCn'nnnn_
Cuts at: 0 677 1221 2382
Size: 677 544 1161
Fragments arranged by size:
1161 677 544
Bsp1286I G_dGCh'C
Cuts at: 0 580 640 752 845 870 880 1006 1273
Size: 580 60 112 93 25 10 126 267
Cuts at: 1273 1463 1643 1766 1900 1934 2087 2185 2382
Size: 190 180 123 134 34 153 98 197
Fragments arranged by size:
580 267 197 190 180 153 134 126
123 112 98 93 60 34 25 10
BspEI T'CCGG_A
Cuts at: 0 1635 2100 2382
Size: 1635 465 282
Fragments arranged by size:
1635 465 282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3 62 767 1430 2382
Size: 3 59 705 663 952
Fragments arranged by size:
952 705 663 59 3
BsrBI CCG'CTC
Cuts at: 0 203 1870 2382
Size: 203 1667 512
Fragments arranged by size:
1667 512 203
BsrFI r'CCGG_y
Cuts at: 0 522 1980 2251 2382
Size: 522 1458 271 131
Fragments arranged by size:
1458 522 271 131
BssHII G'CGCG_C
Cuts at: 0 160 1661 2382
Size: 160 1501 721
Fragments arranged by size:
1501 721 160
BstAPI GCAn_nnn'nTGC
Cuts at: 0 774 2144 2382
Size: 774 1370 238
Fragments arranged by size:
1370 774 238
BstXI CCAn_nnnn'nTGG
Cuts at: 0 388 570 1524 2382
Size: 388 182 954 858
Fragments arranged by size:
954 858 388 182
BtgI C'CryG_G
Cuts at: 0 105 482 837 1265 2111 2382
Size: 105 377 355 428 846 271
Fragments arranged by size:
846 428 377 355 271 105
BtsI GCAGTG_nn'
Cuts at: 0 1517 1702 1940 2382
Size: 1517 185 238 442
Fragments arranged by size:
1517 442 238 185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2188 2223 2382
Size: 2188 35 159
Fragments arranged by size:
2188 159 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 945 2382
Size: 945 1437
EaeI y'GGCC_r
Cuts at: 0 198 287 788 911 1055 1304 1341 1865
Size: 198 89 501 123 144 249 37 524
Cuts at: 1865 2114 2382
Size: 249 268
Fragments arranged by size:
524 501 268 249 249 198 144 123
89 37
EagI C'GGCC_G
Cuts at: 0 198 287 1341 2382
Size: 198 89 1054 1041
Fragments arranged by size:
1054 1041 198 89
EarI CTCTTCn'nnn_
Cuts at: 0 948 2382
Size: 948 1434
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 743 1728 1769 1775 2382
Size: 743 985 41 6 607
Fragments arranged by size:
985 743 607 41 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 451 635 1272 1393 1687 1744 2382
Size: 190 261 184 637 121 294 57 638
Fragments arranged by size:
638 637 294 261 190 184 121 57
EcoO109I rG'GnC_Cy
Cuts at: 0 576 613 733 842 876 877 1050 1347
Size: 576 37 120 109 34 1 173 297
Cuts at: 1347 1568 1804 2025 2382
Size: 221 236 221 357
Fragments arranged by size:
576 357 297 236 221 221 173 120
109 37 34 1
EcoRI G'AATT_C
Cuts at: 0 142 2382
Size: 142 2240
FspI TGC'GCA
Cuts at: 0 1078 1618 2382
Size: 1078 540 764
Fragments arranged by size:
1078 764 540
HaeII r_GCGC'y
Cuts at: 0 131 438 452 797 1410 1469 1723 1759
Size: 131 307 14 345 613 59 254 36
Cuts at: 1759 2004 2047 2063 2382
Size: 245 43 16 319
Fragments arranged by size:
613 345 319 307 254 245 131 59
43 36 16 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 840 872 1201 1233 2216 2248
Size: 187 32 621 32 329 32 983 32
Cuts at: 2248 2382
Size: 134
Fragments arranged by size:
983 621 329 187 134 32 32 32
32
HincII GTy'rAC
Cuts at: 0 117 2382
Size: 117 2265
KasI G'GCGC_C
Cuts at: 0 1406 1465 1755 2043 2382
Size: 1406 59 290 288 339
Fragments arranged by size:
1406 339 290 288 59
KpnI G_GTAC'C
Cuts at: 0 899 2382
Size: 899 1483
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1838 2382
Size: 1838 544
MscI TGG'CCA
Cuts at: 0 1306 2116 2382
Size: 1306 810 266
Fragments arranged by size:
1306 810 266
MslI CAynn'nnrTG
Cuts at: 0 386 1879 2382
Size: 386 1493 503
Fragments arranged by size:
1493 503 386
MspA1I CmG'CkG
Cuts at: 0 107 168 283 484 655 1502 2123 2273
Size: 107 61 115 201 171 847 621 150
Cuts at: 2273 2382
Size: 109
Fragments arranged by size:
847 621 201 171 150 115 109 107
61
NaeI GCC'GGC
Cuts at: 0 1982 2382
Size: 1982 400
NarI GG'CG_CC
Cuts at: 0 1407 1466 1756 2044 2382
Size: 1407 59 290 288 338
Fragments arranged by size:
1407 338 290 288 59
NcoI C'CATG_G
Cuts at: 0 2111 2382
Size: 2111 271
NgoMIV G'CCGG_C
Cuts at: 0 1980 2382
Size: 1980 402
NotI GC'GGCC_GC
Cuts at: 0 287 2382
Size: 287 2095
NruI TCG'CGA
Cuts at: 0 2335 2382
Size: 2335 47
NspI r_CATG'y
Cuts at: 0 66 1476 2382
Size: 66 1410 906
Fragments arranged by size:
1410 906 66
PasI CC'CwG_GG
Cuts at: 0 68 828 862 2241 2382
Size: 68 760 34 1379 141
Fragments arranged by size:
1379 760 141 68 34
PflMI CCAn_nnn'nTGG
Cuts at: 0 80 828 2382
Size: 80 748 1554
Fragments arranged by size:
1554 748 80
PfoI T'CCnGG_A
Cuts at: 0 25 191 2382
Size: 25 166 2191
Fragments arranged by size:
2191 166 25
PpuMI rG'GwC_Cy
Cuts at: 0 613 1804 2025 2382
Size: 613 1191 221 357
Fragments arranged by size:
1191 613 357 221
PspOMI G'GGCC_C
Cuts at: 0 576 841 876 2382
Size: 576 265 35 1506
Fragments arranged by size:
1506 576 265 35
PvuII CAG'CTG
Cuts at: 0 168 1502 2382
Size: 168 1334 880
Fragments arranged by size:
1334 880 168
RsrII CG'GwC_CG
Cuts at: 0 1852 2382
Size: 1852 530
SacII CC_GC'GG
Cuts at: 0 108 485 2382
Size: 108 377 1897
Fragments arranged by size:
1897 377 108
SanDI GG'GwC_CC
Cuts at: 0 613 1804 2382
Size: 613 1191 578
Fragments arranged by size:
1191 613 578
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 575 2245 2382
Size: 575 1670 137
Fragments arranged by size:
1670 575 137
SfoI GGC'GCC
Cuts at: 0 1408 1467 1757 2045 2382
Size: 1408 59 290 288 337
Fragments arranged by size:
1408 337 290 288 59
SgrAI Cr'CCGG_yG
Cuts at: 0 522 2251 2382
Size: 522 1729 131
Fragments arranged by size:
1729 522 131
SmaI CCC'GGG
Cuts at: 0 210 719 902 1559 2069 2382
Size: 210 509 183 657 510 313
Fragments arranged by size:
657 510 509 313 210 183
SphI G_CATG'C
Cuts at: 0 66 2382
Size: 66 2316
StuI AGG'CCT
Cuts at: 0 1630 2382
Size: 1630 752
StyI C'CwwG_G
Cuts at: 0 1913 2111 2308 2382
Size: 1913 198 197 74
Fragments arranged by size:
1913 198 197 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 958 2382
Size: 958 1424
TatI w'GTAC_w
Cuts at: 0 1283 2382
Size: 1283 1099
Tth111I GACn'n_nGTC
Cuts at: 0 1213 2382
Size: 1213 1169
XbaI T'CTAG_A
Cuts at: 0 2221 2382
Size: 2221 161
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1227 2382
Size: 1227 1155
XmaI C'CCGG_G
Cuts at: 0 208 717 900 1557 2067 2382
Size: 208 509 183 657 510 315
Fragments arranged by size:
657 510 509 315 208 183
XmnI GAAnn'nnTTC
Cuts at: 0 1249 1336 2382
Size: 1249 87 1046
Fragments arranged by size:
1249 1046 87
Enzymes that do cut:
AarI Acc65I AclI AcuI AfeI AflIII AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrFI BssHII BstAPI BstXI BtgI BtsI
CspCI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NruI NspI PasI PflMI PfoI PpuMI PspOMI PvuII
RsrII SacII SanDI SfiI SfoI SgrAI SmaI SphI
StuI StyI TaqII TatI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AflII AgeI AhdI AleI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BclI BfrBI BglII
BmtI BpuEI BsaBI BsiWI BsmI BspHI BsrDI BsrGI
BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
HindIII HpaI MfeI MluI NdeI NheI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SmlI SnaBI SpeI SrfI SspI SwaI
TstI XhoI ZraI