Restriction map for
pFN21AE1274
(Linear) (Six-base) MAPSORT of: KIEE1274.seq Check: 4879 from: 1 to: 2133
KIEE1274.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AccI GT'mk_AC
Cuts at: 0 637 2133
Size: 637 1496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1244 1672 1765 2104 2133
Size: 1139 105 428 93 339 29
Fragments arranged by size:
1139 428 339 105 93 29
AflIII A'CryG_T
Cuts at: 0 194 371 1537 2133
Size: 194 177 1166 596
Fragments arranged by size:
1166 596 194 177
AleI CACnn'nnGTG
Cuts at: 0 1225 2133
Size: 1225 908
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7 39 2133
Size: 7 32 2094
Fragments arranged by size:
2094 32 7
AlwNI CAG_nnn'CTG
Cuts at: 0 2093 2127 2133
Size: 2093 34 6
Fragments arranged by size:
2093 34 6
ApoI r'AATT_y
Cuts at: 0 204 610 1282 1297 1630 1832 1848 1863
Size: 204 406 672 15 333 202 16 15
Cuts at: 1863 1903 1937 2133
Size: 40 34 196
Fragments arranged by size:
672 406 333 204 202 196 40 34
16 15 15
AseI AT'TA_AT
Cuts at: 0 2099 2133
Size: 2099 34
AvaI C'yCGr_G
Cuts at: 0 41 1017 1994 2133
Size: 41 976 977 139
Fragments arranged by size:
977 976 139 41
BamHI G'GATC_C
Cuts at: 0 1432 2133
Size: 1432 701
BanI G'GyrC_C
Cuts at: 0 51 664 2133
Size: 51 613 1469
Fragments arranged by size:
1469 613 51
BanII G_rGCy'C
Cuts at: 0 42 2133
Size: 42 2091
BbeI G_GCGC'C
Cuts at: 0 668 2133
Size: 668 1465
BbsI GAAGACnn'nnnn_
Cuts at: 0 837 942 1163 1330 2133
Size: 837 105 221 167 803
Fragments arranged by size:
837 803 221 167 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 136 170 2133
Size: 136 34 1963
Fragments arranged by size:
1963 136 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 666 1323 2133
Size: 666 657 810
Fragments arranged by size:
810 666 657
BclI T'GATC_A
Cuts at: 0 86 1973 2133
Size: 86 1887 160
Fragments arranged by size:
1887 160 86
BfrBI ATG'CAT
Cuts at: 0 964 2133
Size: 964 1169
BglII A'GATC_T
Cuts at: 0 2108 2133
Size: 2108 25
BmgBI CAC'GTC
Cuts at: 0 1505 2133
Size: 1505 628
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1629 2133
Size: 1629 504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1411 1492 1918 2133
Size: 479 932 81 426 215
Fragments arranged by size:
932 479 426 215 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1935 2133
Size: 1935 198
BsaI GGTCTCn'nnnn_
Cuts at: 0 2055 2133
Size: 2055 78
BsaHI Gr'CG_yC
Cuts at: 0 665 2133
Size: 665 1468
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 760 790 2133
Size: 760 30 1343
Fragments arranged by size:
1343 760 30
BseYI C'CCAG_C
Cuts at: 0 1259 2133
Size: 1259 874
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 451 2068 2133
Size: 52 399 1617 65
Fragments arranged by size:
1617 399 65 52
BsmI GAATG_Cn'
Cuts at: 0 159 964 966 1973 2133
Size: 159 805 2 1007 160
Fragments arranged by size:
1007 805 160 159 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1739 2133
Size: 1739 394
Bsp1286I G_dGCh'C
Cuts at: 0 42 2133
Size: 42 2091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 579 2133
Size: 579 1554
BsrBI CCG'CTC
Cuts at: 0 150 162 2133
Size: 150 12 1971
Fragments arranged by size:
1971 150 12
BsrFI r'CCGG_y
Cuts at: 0 219 2133
Size: 219 1914
BsrGI T'GTAC_A
Cuts at: 0 137 2133
Size: 137 1996
BstXI CCAn_nnnn'nTGG
Cuts at: 0 945 2133
Size: 945 1188
BstYI r'GATC_y
Cuts at: 0 867 1432 1647 2108 2133
Size: 867 565 215 461 25
Fragments arranged by size:
867 565 461 215 25
BtgI C'CryG_G
Cuts at: 0 1795 2036 2133
Size: 1795 241 97
Fragments arranged by size:
1795 241 97
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 46 81 2133
Size: 46 35 2052
Fragments arranged by size:
2052 46 35
DraI TTT'AAA
Cuts at: 0 1242 2133
Size: 1242 891
EaeI y'GGCC_r
Cuts at: 0 1247 1792 2033 2133
Size: 1247 545 241 100
Fragments arranged by size:
1247 545 241 100
EarI CTCTTCn'nnn_
Cuts at: 0 320 1003 1119 2133
Size: 320 683 116 1014
Fragments arranged by size:
1014 683 320 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1139 1244 1411 1492 1672 1765 1918
Size: 479 660 105 167 81 180 93 153
Cuts at: 1918 2104 2133
Size: 186 29
Fragments arranged by size:
660 479 186 180 167 153 105 93
81 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 451 2133
Size: 451 1682
EcoO109I rG'GnC_Cy
Cuts at: 0 1006 1466 2133
Size: 1006 460 667
Fragments arranged by size:
1006 667 460
EcoRI G'AATT_C
Cuts at: 0 1282 2133
Size: 1282 851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1225 2040 2133
Size: 1225 815 93
Fragments arranged by size:
1225 815 93
HaeII r_GCGC'y
Cuts at: 0 414 668 1489 2046 2133
Size: 414 254 821 557 87
Fragments arranged by size:
821 557 414 254 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 673 705 1359 1391 2133
Size: 673 32 654 32 742
Fragments arranged by size:
742 673 654 32 32
HincII GTy'rAC
Cuts at: 0 978 2133
Size: 978 1155
HindIII A'AGCT_T
Cuts at: 0 1236 1720 2133
Size: 1236 484 413
Fragments arranged by size:
1236 484 413
KasI G'GCGC_C
Cuts at: 0 664 2133
Size: 664 1469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 777 2133
Size: 777 1356
MscI TGG'CCA
Cuts at: 0 1249 1794 2035 2133
Size: 1249 545 241 98
Fragments arranged by size:
1249 545 241 98
MslI CAynn'nnrTG
Cuts at: 0 199 1225 1363 2133
Size: 199 1026 138 770
Fragments arranged by size:
1026 770 199 138
MspA1I CmG'CkG
Cuts at: 0 607 1497 2057 2133
Size: 607 890 560 76
Fragments arranged by size:
890 607 560 76
NarI GG'CG_CC
Cuts at: 0 665 2133
Size: 665 1468
NcoI C'CATG_G
Cuts at: 0 2036 2133
Size: 2036 97
NsiI A_TGCA'T
Cuts at: 0 966 2133
Size: 966 1167
NspI r_CATG'y
Cuts at: 0 198 910 1541 1768 2133
Size: 198 712 631 227 365
Fragments arranged by size:
712 631 365 227 198
PasI CC'CwG_GG
Cuts at: 0 9 2133
Size: 9 2124
PciI A'CATG_T
Cuts at: 0 194 1537 2133
Size: 194 1343 596
Fragments arranged by size:
1343 596 194
PflMI CCAn_nnn'nTGG
Cuts at: 0 80 222 756 2133
Size: 80 142 534 1377
Fragments arranged by size:
1377 534 142 80
PpuMI rG'GwC_Cy
Cuts at: 0 1006 1466 2133
Size: 1006 460 667
Fragments arranged by size:
1006 667 460
PspXI vC'TCGA_Gb
Cuts at: 0 1994 2133
Size: 1994 139
PstI C_TGCA'G
Cuts at: 0 877 2133
Size: 877 1256
PvuII CAG'CTG
Cuts at: 0 607 1497 2133
Size: 607 890 636
Fragments arranged by size:
890 636 607
RsrII CG'GwC_CG
Cuts at: 0 164 2133
Size: 164 1969
SanDI GG'GwC_CC
Cuts at: 0 1466 2133
Size: 1466 667
ScaI AGT'ACT
Cuts at: 0 592 2133
Size: 592 1541
SfcI C'TryA_G
Cuts at: 0 873 2133
Size: 873 1260
SfoI GGC'GCC
Cuts at: 0 666 2133
Size: 666 1467
SmaI CCC'GGG
Cuts at: 0 43 2133
Size: 43 2090
SmlI C'TyrA_G
Cuts at: 0 1914 1994 2133
Size: 1914 80 139
Fragments arranged by size:
1914 139 80
SspI AAT'ATT
Cuts at: 0 1887 2133
Size: 1887 246
StyI C'CwwG_G
Cuts at: 0 74 447 763 1002 2036 2133
Size: 74 373 316 239 1034 97
Fragments arranged by size:
1034 373 316 239 97 74
TatI w'GTAC_w
Cuts at: 0 137 590 716 1526 2133
Size: 137 453 126 810 607
Fragments arranged by size:
810 607 453 137 126
XhoI C'TCGA_G
Cuts at: 0 1994 2133
Size: 1994 139
XmaI C'CCGG_G
Cuts at: 0 41 2133
Size: 41 2092
XmnI GAAnn'nnTTC
Cuts at: 0 314 964 1641 1810 1867 2133
Size: 314 650 677 169 57 266
Fragments arranged by size:
677 650 314 266 169 57
Enzymes that do cut:
AccI AcuI AflIII AleI AloI AlwNI ApoI AseI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BmgBI BplI BpmI BpuEI BsaI
BsaHI BsaXI BseYI BsgI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrFI BsrGI BstXI BstYI BtgI CspCI DraI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I
NarI NcoI NsiI NspI PasI PciI PflMI PpuMI
PspXI PstI PvuII RsrII SanDI ScaI SfcI SfoI
SmaI SmlI SspI StyI TatI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BbvCI
BglI BlpI Bme1580I BmrI BmtI Bpu10I BsaAI BsaBI
BsaWI BseRI BsiEI BsiHKAI BsiWI BspEI BspHI BsrDI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI DraIII DrdI EagI EciI EcoICRI EcoRV
FseI FspI FspAI HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI PacI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PvuI SacI
SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
Tth111I XbaI XcmI ZraI