Restriction map for
pFN21AE1263
(Linear) (Six-base) MAPSORT of: KIEE1263.seq Check: 3110 from: 1 to: 1821
KIEE1263.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
Acc65I G'GTAC_C
Cuts at: 0 927 1821
Size: 927 894
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1608 1821
Size: 505 1103 213
Fragments arranged by size:
1103 505 213
AhdI GACnn_n'nnGTC
Cuts at: 0 859 1821
Size: 859 962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1819 1821
Size: 1819 2
AlwNI CAG_nnn'CTG
Cuts at: 0 483 567 937 1657 1821
Size: 483 84 370 720 164
Fragments arranged by size:
720 483 370 164 84
ApaLI G'TGCA_C
Cuts at: 0 1580 1821
Size: 1580 241
ApoI r'AATT_y
Cuts at: 0 1117 1409 1821
Size: 1117 292 412
Fragments arranged by size:
1117 412 292
BamHI G'GATC_C
Cuts at: 0 1345 1821
Size: 1345 476
BanI G'GyrC_C
Cuts at: 0 65 927 1821
Size: 65 862 894
Fragments arranged by size:
894 862 65
BanII G_rGCy'C
Cuts at: 0 1508 1669 1821
Size: 1508 161 152
Fragments arranged by size:
1508 161 152
BbeI G_GCGC'C
Cuts at: 0 69 1821
Size: 69 1752
BbsI GAAGACnn'nnnn_
Cuts at: 0 942 1614 1821
Size: 942 672 207
Fragments arranged by size:
942 672 207
BbvCI CC'TCA_GC
Cuts at: 0 1661 1821
Size: 1661 160
BciVI GTATCCnnnnn_n'
Cuts at: 0 888 1821
Size: 888 933
BclI T'GATC_A
Cuts at: 0 744 1821
Size: 744 1077
BglI GCCn_nnn'nGGC
Cuts at: 0 1634 1821
Size: 1634 187
BglII A'GATC_T
Cuts at: 0 138 1821
Size: 138 1683
Bme1580I G_kGCm'C
Cuts at: 0 1584 1815 1821
Size: 1584 231 6
Fragments arranged by size:
1584 231 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 868 1821
Size: 868 953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 621 836 1037 1701 1821
Size: 442 179 215 201 664 120
Fragments arranged by size:
664 442 215 201 179 120
Bpu10I CC'TnA_GC
Cuts at: 0 1510 1661 1821
Size: 1510 151 160
Fragments arranged by size:
1510 160 151
BsaI GGTCTCn'nnnn_
Cuts at: 0 751 1821
Size: 751 1070
BsaAI yAC'GTr
Cuts at: 0 1644 1821
Size: 1644 177
BsaBI GATnn'nnATC
Cuts at: 0 899 1190 1821
Size: 899 291 631
Fragments arranged by size:
899 631 291
BsaHI Gr'CG_yC
Cuts at: 0 66 1821
Size: 66 1755
BsaWI w'CCGG_w
Cuts at: 0 1286 1821
Size: 1286 535
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 415 445 1821
Size: 415 30 1376
Fragments arranged by size:
1376 415 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 1821
Size: 112 1709
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 1353 1821
Size: 1003 350 468
Fragments arranged by size:
1003 468 350
BsiEI CG_ry'CG
Cuts at: 0 1287 1821
Size: 1287 534
BsiHKAI G_wGCw'C
Cuts at: 0 1508 1584 1691 1821
Size: 1508 76 107 130
Fragments arranged by size:
1508 130 107 76
BsmBI CGTCTCn'nnnn_
Cuts at: 0 418 846 1821
Size: 418 428 975
Fragments arranged by size:
975 428 418
Bsp1286I G_dGCh'C
Cuts at: 0 1508 1584 1669 1691 1815 1821
Size: 1508 76 85 22 124 6
Fragments arranged by size:
1508 124 85 76 22 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 449 1821
Size: 449 1372
BsrDI GCAATG_nn'
Cuts at: 0 1362 1384 1821
Size: 1362 22 437
Fragments arranged by size:
1362 437 22
BsrFI r'CCGG_y
Cuts at: 0 74 1821
Size: 74 1747
BssSI C'ACGA_G
Cuts at: 0 199 1821
Size: 199 1622
BstAPI GCAn_nnn'nTGC
Cuts at: 0 391 1322 1657 1821
Size: 391 931 335 164
Fragments arranged by size:
931 391 335 164
BstEII G'GTnAC_C
Cuts at: 0 570 1821
Size: 570 1251
BstXI CCAn_nnnn'nTGG
Cuts at: 0 582 1821
Size: 582 1239
BstYI r'GATC_y
Cuts at: 0 138 1132 1305 1345 1821
Size: 138 994 173 40 476
Fragments arranged by size:
994 476 173 138 40
ClaI AT'CG_AT
Cuts at: 0 1194 1821
Size: 1194 627
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 288 323 1821
Size: 288 35 1498
Fragments arranged by size:
1498 288 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 721 1821
Size: 721 1100
EaeI y'GGCC_r
Cuts at: 0 1010 1821
Size: 1010 811
EarI CTCTTCn'nnn_
Cuts at: 0 548 749 870 1821
Size: 548 201 121 951
Fragments arranged by size:
951 548 201 121
EcoICRI GAG'CTC
Cuts at: 0 1506 1821
Size: 1506 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 505 621 836 1037 1608 1701 1821
Size: 442 63 116 215 201 571 93 120
Fragments arranged by size:
571 442 215 201 120 116 93 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 815 1821
Size: 815 1006
FspI TGC'GCA
Cuts at: 0 445 1821
Size: 445 1376
HaeII r_GCGC'y
Cuts at: 0 45 69 1821
Size: 45 24 1752
Fragments arranged by size:
1752 45 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 845 877 1124 1156 1414 1446 1821
Size: 845 32 247 32 258 32 375
Fragments arranged by size:
845 375 258 247 32 32 32
HincII GTy'rAC
Cuts at: 0 975 1821
Size: 975 846
HindIII A'AGCT_T
Cuts at: 0 451 1039 1821
Size: 451 588 782
Fragments arranged by size:
782 588 451
KasI G'GCGC_C
Cuts at: 0 65 1821
Size: 65 1756
KpnI G_GTAC'C
Cuts at: 0 931 1821
Size: 931 890
MscI TGG'CCA
Cuts at: 0 1012 1821
Size: 1012 809
MspA1I CmG'CkG
Cuts at: 0 22 35 354 1821
Size: 22 13 319 1467
Fragments arranged by size:
1467 319 22 13
NarI GG'CG_CC
Cuts at: 0 66 1821
Size: 66 1755
PflMI CCAn_nnn'nTGG
Cuts at: 0 567 1821
Size: 567 1254
PfoI T'CCnGG_A
Cuts at: 0 210 637 1821
Size: 210 427 1184
Fragments arranged by size:
1184 427 210
PstI C_TGCA'G
Cuts at: 0 83 113 509 662 1821
Size: 83 30 396 153 1159
Fragments arranged by size:
1159 396 153 83 30
SacI G_AGCT'C
Cuts at: 0 1508 1821
Size: 1508 313
SfcI C'TryA_G
Cuts at: 0 79 109 166 505 658 1821
Size: 79 30 57 339 153 1163
Fragments arranged by size:
1163 339 153 79 57 30
SfoI GGC'GCC
Cuts at: 0 67 1821
Size: 67 1754
StuI AGG'CCT
Cuts at: 0 821 1821
Size: 821 1000
StyI C'CwwG_G
Cuts at: 0 524 1821
Size: 524 1297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 415 447 1717 1749 1821
Size: 415 32 1270 32 72
Fragments arranged by size:
1270 415 72 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1020 1743 1821
Size: 1020 723 78
Fragments arranged by size:
1020 723 78
XmnI GAAnn'nnTTC
Cuts at: 0 1709 1821
Size: 1709 112
Enzymes that do cut:
Acc65I AcuI AhdI AlfI AlwNI ApaLI ApoI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI ClaI CspCI DrdI EaeI EarI EcoICRI Eco57MI
EcoNI FspI HaeII Hin4I HincII HindIII KasI KpnI
MscI MspA1I NarI PflMI PfoI PstI SacI SfcI
SfoI StuI StyI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BcgI BfrBI BlpI BmgBI BmtI BplI BpuEI
BseYI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII
BstBI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
EagI EciI EcoO109I EcoRI EcoRV FalI FseI FspAI
HpaI MfeI MluI MmeI MslI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TatI Tth111I XbaI XhoI XmaI ZraI