Restriction map for
pFN21AE1261
(Linear) (Six-base) MAPSORT of: KIEE1261.seq Check: 6296 from: 1 to: 1812
KIEE1261.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
Acc65I G'GTAC_C
Cuts at: 0 1205 1812
Size: 1205 607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 1812
Size: 393 1419
AhdI GACnn_n'nnGTC
Cuts at: 0 252 1812
Size: 252 1560
ApoI r'AATT_y
Cuts at: 0 278 1027 1812
Size: 278 749 785
Fragments arranged by size:
785 749 278
AvrII C'CTAG_G
Cuts at: 0 171 1086 1812
Size: 171 915 726
Fragments arranged by size:
915 726 171
BanI G'GyrC_C
Cuts at: 0 1205 1236 1812
Size: 1205 31 576
Fragments arranged by size:
1205 576 31
BanII G_rGCy'C
Cuts at: 0 391 803 1812
Size: 391 412 1009
Fragments arranged by size:
1009 412 391
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 1812
Size: 248 1564
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 327 361 1812
Size: 327 34 1451
Fragments arranged by size:
1451 327 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 360 390 1366 1504 1812
Size: 360 30 976 138 308
Fragments arranged by size:
976 360 308 138 30
BglI GCCn_nnn'nGGC
Cuts at: 0 835 1489 1812
Size: 835 654 323
Fragments arranged by size:
835 654 323
Bme1580I G_kGCm'C
Cuts at: 0 666 1812
Size: 666 1146
BmrI ACTGGGnnnn_n'
Cuts at: 0 475 1060 1324 1516 1786 1812
Size: 475 585 264 192 270 26
Fragments arranged by size:
585 475 270 264 192 26
Bpu10I CC'TnA_GC
Cuts at: 0 797 1812
Size: 797 1015
BsaI GGTCTCn'nnnn_
Cuts at: 0 896 1812
Size: 896 916
BsaAI yAC'GTr
Cuts at: 0 104 1812
Size: 104 1708
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 686 716 1812
Size: 686 30 1096
Fragments arranged by size:
1096 686 30
BseYI C'CCAG_C
Cuts at: 0 3 160 494 1043 1479 1610 1812
Size: 3 157 334 549 436 131 202
Fragments arranged by size:
549 436 334 202 157 131 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 486 844 962 1812
Size: 75 411 358 118 850
Fragments arranged by size:
850 411 358 118 75
Bsp1286I G_dGCh'C
Cuts at: 0 391 666 803 1812
Size: 391 275 137 1009
Fragments arranged by size:
1009 391 275 137
BspHI T'CATG_A
Cuts at: 0 119 1812
Size: 119 1693
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 175 984 1812
Size: 175 809 828
Fragments arranged by size:
828 809 175
BsrBI CCG'CTC
Cuts at: 0 737 1812
Size: 737 1075
BssSI C'ACGA_G
Cuts at: 0 665 1812
Size: 665 1147
BstAPI GCAn_nnn'nTGC
Cuts at: 0 460 1812
Size: 460 1352
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1229 1295 1487 1812
Size: 1229 66 192 325
Fragments arranged by size:
1229 325 192 66
BstYI r'GATC_y
Cuts at: 0 266 303 1256 1812
Size: 266 37 953 556
Fragments arranged by size:
953 556 266 37
Bsu36I CC'TnA_GG
Cuts at: 0 1368 1812
Size: 1368 444
BtgI C'CryG_G
Cuts at: 0 383 416 913 1812
Size: 383 33 497 899
Fragments arranged by size:
899 497 383 33
BtsI GCAGTG_nn'
Cuts at: 0 87 1812
Size: 87 1725
DraI TTT'AAA
Cuts at: 0 647 1193 1812
Size: 647 546 619
Fragments arranged by size:
647 619 546
DraIII CAC_nnn'GTG
Cuts at: 0 104 509 1812
Size: 104 405 1303
Fragments arranged by size:
1303 405 104
EaeI y'GGCC_r
Cuts at: 0 812 910 1812
Size: 812 98 902
Fragments arranged by size:
902 812 98
EarI CTCTTCn'nnn_
Cuts at: 0 969 1676 1812
Size: 969 707 136
Fragments arranged by size:
969 707 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 1812
Size: 393 1419
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1354 1675 1812
Size: 1354 321 137
Fragments arranged by size:
1354 321 137
EcoO109I rG'GnC_Cy
Cuts at: 0 164 1304 1592 1812
Size: 164 1140 288 220
Fragments arranged by size:
1140 288 220 164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 322 1812
Size: 322 1490
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 334 366 686 718 1364 1396
Size: 238 32 64 32 320 32 646 32
Cuts at: 1396 1812
Size: 416
Fragments arranged by size:
646 416 320 238 64 32 32 32
32
HincII GTy'rAC
Cuts at: 0 783 1079 1812
Size: 783 296 733
Fragments arranged by size:
783 733 296
HpaI GTT'AAC
Cuts at: 0 783 1079 1812
Size: 783 296 733
Fragments arranged by size:
783 733 296
KpnI G_GTAC'C
Cuts at: 0 1209 1812
Size: 1209 603
MscI TGG'CCA
Cuts at: 0 814 912 1812
Size: 814 98 900
Fragments arranged by size:
900 814 98
MslI CAynn'nnrTG
Cuts at: 0 1624 1812
Size: 1624 188
MspA1I CmG'CkG
Cuts at: 0 674 1812
Size: 674 1138
NcoI C'CATG_G
Cuts at: 0 383 416 913 1812
Size: 383 33 497 899
Fragments arranged by size:
899 497 383 33
NdeI CA'TA_TG
Cuts at: 0 631 1812
Size: 631 1181
NspI r_CATG'y
Cuts at: 0 18 781 1533 1812
Size: 18 763 752 279
Fragments arranged by size:
763 752 279 18
PasI CC'CwG_GG
Cuts at: 0 71 1812
Size: 71 1741
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 168 200 1812
Size: 168 32 1612
Fragments arranged by size:
1612 168 32
PpuMI rG'GwC_Cy
Cuts at: 0 164 1812
Size: 164 1648
PsiI TTA'TAA
Cuts at: 0 575 1546 1812
Size: 575 971 266
Fragments arranged by size:
971 575 266
PstI C_TGCA'G
Cuts at: 0 1590 1812
Size: 1590 222
ScaI AGT'ACT
Cuts at: 0 424 1562 1812
Size: 424 1138 250
Fragments arranged by size:
1138 424 250
SfcI C'TryA_G
Cuts at: 0 426 1421 1586 1812
Size: 426 995 165 226
Fragments arranged by size:
995 426 226 165
SspI AAT'ATT
Cuts at: 0 1099 1471 1812
Size: 1099 372 341
Fragments arranged by size:
1099 372 341
StuI AGG'CCT
Cuts at: 0 1054 1812
Size: 1054 758
StyI C'CwwG_G
Cuts at: 0 171 320 383 416 809 913 1086 1812
Size: 171 149 63 33 393 104 173 726
Fragments arranged by size:
726 393 173 171 149 104 63 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 109 1178 1812
Size: 109 1069 634
Fragments arranged by size:
1069 634 109
TatI w'GTAC_w
Cuts at: 0 181 422 1311 1560 1812
Size: 181 241 889 249 252
Fragments arranged by size:
889 252 249 241 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1425 1457 1812
Size: 1425 32 355
Fragments arranged by size:
1425 355 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1799 1812
Size: 1799 13
Enzymes that do cut:
Acc65I AcuI AhdI ApoI AvrII BanI BanII BbsI
BcgI BciVI BglI Bme1580I BmrI Bpu10I BsaI BsaAI
BsaXI BseYI BsgI Bsp1286I BspHI BspMI BsrBI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I FalI Hin4I HincII
HpaI KpnI MscI MslI MspA1I NcoI NdeI NspI
PasI PpiI PpuMI PsiI PstI ScaI SfcI SspI
StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI BaeI BamHI BbeI BbvCI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaBI
BsaHI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I
BtgZI ClaI CspCI DrdI EagI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII KasI MfeI
MluI MmeI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI