Restriction map for
pFN21AE1260
(Linear) (Six-base) MAPSORT of: KIEE1260.seq Check: 6856 from: 1 to: 1785
KIEE1260.orf
With 182 enzymes: SgfI *
July 3, 2012 16:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 133 1523 1785
Size: 25 108 1390 262
Fragments arranged by size:
1390 262 108 25
AfeI AGC'GCT
Cuts at: 0 681 1249 1785
Size: 681 568 536
Fragments arranged by size:
681 568 536
AflIII A'CryG_T
Cuts at: 0 599 1472 1785
Size: 599 873 313
Fragments arranged by size:
873 599 313
AleI CACnn'nnGTG
Cuts at: 0 1571 1785
Size: 1571 214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1008 1785
Size: 1008 777
AlwNI CAG_nnn'CTG
Cuts at: 0 1406 1785
Size: 1406 379
ApaI G_GGCC'C
Cuts at: 0 440 1313 1785
Size: 440 873 472
Fragments arranged by size:
873 472 440
ApaLI G'TGCA_C
Cuts at: 0 1476 1785
Size: 1476 309
AvaI C'yCGr_G
Cuts at: 0 433 1785
Size: 433 1352
AvrII C'CTAG_G
Cuts at: 0 1063 1785
Size: 1063 722
BamHI G'GATC_C
Cuts at: 0 800 1118 1454 1785
Size: 800 318 336 331
Fragments arranged by size:
800 336 331 318
BanI G'GyrC_C
Cuts at: 0 115 418 484 670 811 868 1069 1785
Size: 115 303 66 186 141 57 201 716
Fragments arranged by size:
716 303 201 186 141 115 66 57
BanII G_rGCy'C
Cuts at: 0 440 1313 1765 1785
Size: 440 873 452 20
Fragments arranged by size:
873 452 440 20
BbeI G_GCGC'C
Cuts at: 0 119 422 488 872 1785
Size: 119 303 66 384 913
Fragments arranged by size:
913 384 303 119 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1209 1243 1785
Size: 1209 34 542
Fragments arranged by size:
1209 542 34
BfrBI ATG'CAT
Cuts at: 0 636 1133 1785
Size: 636 497 652
Fragments arranged by size:
652 636 497
BglI GCCn_nnn'nGGC
Cuts at: 0 313 717 1785
Size: 313 404 1068
Fragments arranged by size:
1068 404 313
BlpI GC'TnA_GC
Cuts at: 0 185 1714 1785
Size: 185 1529 71
Fragments arranged by size:
1529 185 71
Bme1580I G_kGCm'C
Cuts at: 0 440 631 673 767 1072 1313 1480 1785
Size: 440 191 42 94 305 241 167 305
Fragments arranged by size:
440 305 305 241 191 167 94 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 1515 1785
Size: 1515 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1676 1785
Size: 1676 109
Bpu10I CC'TnA_GC
Cuts at: 0 130 1785
Size: 130 1655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1785
Size: 88 1697
BsaI GGTCTCn'nnnn_
Cuts at: 0 8 130 325 1785
Size: 8 122 195 1460
Fragments arranged by size:
1460 195 122 8
BsaAI yAC'GTr
Cuts at: 0 1473 1557 1785
Size: 1473 84 228
Fragments arranged by size:
1473 228 84
BsaHI Gr'CG_yC
Cuts at: 0 116 419 485 869 1196 1785
Size: 116 303 66 384 327 589
Fragments arranged by size:
589 384 327 303 116 66
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 219 261 270 526 1704 1785
Size: 219 42 9 256 1178 81
Fragments arranged by size:
1178 256 219 81 42 9
BseYI C'CCAG_C
Cuts at: 0 48 1241 1448 1532 1709 1785
Size: 48 1193 207 84 177 76
Fragments arranged by size:
1193 207 177 84 76 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 735 880 1206 1296 1785
Size: 631 104 145 326 90 489
Fragments arranged by size:
631 489 326 145 104 90
BsiEI CG_ry'CG
Cuts at: 0 299 689 1785
Size: 299 390 1096
Fragments arranged by size:
1096 390 299
BsiHKAI G_wGCw'C
Cuts at: 0 1480 1785
Size: 1480 305
Bsp1286I G_dGCh'C
Cuts at: 0 440 631 673 767 1072 1313 1480 1765
Size: 440 191 42 94 305 241 167 285
Cuts at: 1765 1785
Size: 20
Fragments arranged by size:
440 305 285 241 191 167 94 42
20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 65 737 1785
Size: 65 672 1048
Fragments arranged by size:
1048 672 65
BsrBI CCG'CTC
Cuts at: 0 102 561 1785
Size: 102 459 1224
Fragments arranged by size:
1224 459 102
BsrDI GCAATG_nn'
Cuts at: 0 1133 1785
Size: 1133 652
BsrFI r'CCGG_y
Cuts at: 0 246 333 348 659 1577 1785
Size: 246 87 15 311 918 208
Fragments arranged by size:
918 311 246 208 87 15
BssHII G'CGCG_C
Cuts at: 0 134 180 1785
Size: 134 46 1605
Fragments arranged by size:
1605 134 46
BstAPI GCAn_nnn'nTGC
Cuts at: 0 654 1785
Size: 654 1131
BstYI r'GATC_y
Cuts at: 0 800 1118 1454 1785
Size: 800 318 336 331
Fragments arranged by size:
800 336 331 318
BtgI C'CryG_G
Cuts at: 0 119 211 1785
Size: 119 92 1574
Fragments arranged by size:
1574 119 92
BtsI GCAGTG_nn'
Cuts at: 0 643 990 1435 1512 1785
Size: 643 347 445 77 273
Fragments arranged by size:
643 445 347 273 77
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 432 467 1760 1785
Size: 432 35 1293 25
Fragments arranged by size:
1293 432 35 25
EaeI y'GGCC_r
Cuts at: 0 296 534 686 1509 1785
Size: 296 238 152 823 276
Fragments arranged by size:
823 296 276 238 152
EagI C'GGCC_G
Cuts at: 0 296 686 1785
Size: 296 390 1099
Fragments arranged by size:
1099 390 296
EarI CTCTTCn'nnn_
Cuts at: 0 82 152 261 1298 1785
Size: 82 70 109 1037 487
Fragments arranged by size:
1037 487 109 82 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 182 222 255 264 1785
Size: 182 40 33 9 1521
Fragments arranged by size:
1521 182 40 33 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 133 1523 1676 1785
Size: 25 108 1390 153 109
Fragments arranged by size:
1390 153 109 108 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 86 830 1785
Size: 86 744 955
Fragments arranged by size:
955 744 86
EcoO109I rG'GnC_Cy
Cuts at: 0 726 1785
Size: 726 1059
EcoRV GAT'ATC
Cuts at: 0 1059 1785
Size: 1059 726
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 856 1388 1785
Size: 856 532 397
Fragments arranged by size:
856 532 397
HaeII r_GCGC'y
Cuts at: 0 119 422 488 683 872 1251 1785
Size: 119 303 66 195 189 379 534
Fragments arranged by size:
534 379 303 195 189 119 66
KasI G'GCGC_C
Cuts at: 0 115 418 484 868 1785
Size: 115 303 66 384 917
Fragments arranged by size:
917 384 303 115 66
MscI TGG'CCA
Cuts at: 0 536 1785
Size: 536 1249
MslI CAynn'nnrTG
Cuts at: 0 1571 1785
Size: 1571 214
MspA1I CmG'CkG
Cuts at: 0 113 121 213 301 345 373 393 500
Size: 113 8 92 88 44 28 20 107
Cuts at: 500 1452 1713 1728 1785
Size: 952 261 15 57
Fragments arranged by size:
952 261 113 107 92 88 57 44
28 20 15 8
NaeI GCC'GGC
Cuts at: 0 248 661 1785
Size: 248 413 1124
Fragments arranged by size:
1124 413 248
NarI GG'CG_CC
Cuts at: 0 116 419 485 869 1785
Size: 116 303 66 384 916
Fragments arranged by size:
916 384 303 116 66
NgoMIV G'CCGG_C
Cuts at: 0 246 659 1785
Size: 246 413 1126
Fragments arranged by size:
1126 413 246
NotI GC'GGCC_GC
Cuts at: 0 296 686 1785
Size: 296 390 1099
Fragments arranged by size:
1099 390 296
NruI TCG'CGA
Cuts at: 0 737 1785
Size: 737 1048
NsiI A_TGCA'T
Cuts at: 0 638 1135 1785
Size: 638 497 650
Fragments arranged by size:
650 638 497
NspI r_CATG'y
Cuts at: 0 603 1785
Size: 603 1182
PciI A'CATG_T
Cuts at: 0 599 1785
Size: 599 1186
PmlI CAC'GTG
Cuts at: 0 1473 1557 1785
Size: 1473 84 228
Fragments arranged by size:
1473 228 84
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 398 430 1785
Size: 398 32 1355
Fragments arranged by size:
1355 398 32
PpuMI rG'GwC_Cy
Cuts at: 0 726 1785
Size: 726 1059
PspOMI G'GGCC_C
Cuts at: 0 436 1309 1785
Size: 436 873 476
Fragments arranged by size:
873 476 436
PstI C_TGCA'G
Cuts at: 0 62 505 1205 1785
Size: 62 443 700 580
Fragments arranged by size:
700 580 443 62
PvuII CAG'CTG
Cuts at: 0 345 1713 1785
Size: 345 1368 72
Fragments arranged by size:
1368 345 72
SacII CC_GC'GG
Cuts at: 0 122 214 1785
Size: 122 92 1571
Fragments arranged by size:
1571 122 92
ScaI AGT'ACT
Cuts at: 0 759 1785
Size: 759 1026
SfcI C'TryA_G
Cuts at: 0 58 501 922 1201 1785
Size: 58 443 421 279 584
Fragments arranged by size:
584 443 421 279 58
SfoI GGC'GCC
Cuts at: 0 117 420 486 870 1785
Size: 117 303 66 384 915
Fragments arranged by size:
915 384 303 117 66
SmaI CCC'GGG
Cuts at: 0 435 1785
Size: 435 1350
SmlI C'TyrA_G
Cuts at: 0 103 1785
Size: 103 1682
StyI C'CwwG_G
Cuts at: 0 401 831 1063 1785
Size: 401 430 232 722
Fragments arranged by size:
722 430 401 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1255 1462 1723 1785
Size: 1255 207 261 62
Fragments arranged by size:
1255 261 207 62
TatI w'GTAC_w
Cuts at: 0 757 1022 1785
Size: 757 265 763
Fragments arranged by size:
763 757 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 1785
Size: 1168 32 585
Fragments arranged by size:
1168 585 32
XmaI C'CCGG_G
Cuts at: 0 433 1785
Size: 433 1352
XmnI GAAnn'nnTTC
Cuts at: 0 1036 1785
Size: 1036 749
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI
AvaI AvrII BamHI BanI BanII BbeI BcgI BfrBI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstYI BtgI
BtsI CspCI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII KasI MscI MslI MspA1I
NaeI NarI NgoMIV NotI NruI NsiI NspI PciI
PmlI PpiI PpuMI PspOMI PstI PvuII SacII ScaI
SfcI SfoI SmaI SmlI StyI TaqII TatI TstI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AloI ApoI AscI AseI AsiSI BaeI BbsI BbvCI
BciVI BclI BglII BmgBI BmtI BplI BsaBI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EcoICRI EcoRI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NheI PacI PasI PflMI PfoI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI ZraI