Restriction map for
pFN21AE1249
(Linear) (Six-base) MAPSORT of: KIEE1249.seq Check: 4070 from: 1 to: 1482
KIEE1249.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 347 1482
Size: 347 1135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 1482
Size: 1021 461
AfeI AGC'GCT
Cuts at: 0 1006 1482
Size: 1006 476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 589 1482
Size: 589 893
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1144 1176 1482
Size: 1144 32 306
Fragments arranged by size:
1144 306 32
AlwNI CAG_nnn'CTG
Cuts at: 0 339 477 1254 1482
Size: 339 138 777 228
Fragments arranged by size:
777 339 228 138
ApaI G_GGCC'C
Cuts at: 0 259 1450 1482
Size: 259 1191 32
Fragments arranged by size:
1191 259 32
AvaI C'yCGr_G
Cuts at: 0 72 121 129 1441 1482
Size: 72 49 8 1312 41
Fragments arranged by size:
1312 72 49 41 8
BamHI G'GATC_C
Cuts at: 0 1040 1482
Size: 1040 442
BanI G'GyrC_C
Cuts at: 0 117 125 190 291 307 634 852 1397
Size: 117 8 65 101 16 327 218 545
Cuts at: 1397 1456 1482
Size: 59 26
Fragments arranged by size:
545 327 218 117 101 65 59 26
16 8
BanII G_rGCy'C
Cuts at: 0 73 259 1364 1430 1450 1482
Size: 73 186 1105 66 20 32
Fragments arranged by size:
1105 186 73 66 32 20
BbeI G_GCGC'C
Cuts at: 0 121 129 194 295 638 856 1401 1460
Size: 121 8 65 101 343 218 545 59
Cuts at: 1460 1482
Size: 22
Fragments arranged by size:
545 343 218 121 101 65 59 22
8
BbvCI CC'TCA_GC
Cuts at: 0 1421 1482
Size: 1421 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1269 1303 1482
Size: 1269 34 179
Fragments arranged by size:
1269 179 34
BglI GCCn_nnn'nGGC
Cuts at: 0 26 148 289 305 692 1454 1482
Size: 26 122 141 16 387 762 28
Fragments arranged by size:
762 387 141 122 28 26 16
BlpI GC'TnA_GC
Cuts at: 0 7 67 1482
Size: 7 60 1415
Fragments arranged by size:
1415 60 7
Bme1580I G_kGCm'C
Cuts at: 0 259 1063 1450 1482
Size: 259 804 387 32
Fragments arranged by size:
804 387 259 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1482
Size: 34 1448
Bpu10I CC'TnA_GC
Cuts at: 0 1421 1482
Size: 1421 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 1114 1482
Size: 1114 368
BsaBI GATnn'nnATC
Cuts at: 0 1291 1482
Size: 1291 191
BsaHI Gr'CG_yC
Cuts at: 0 118 126 191 292 635 853 1398 1457
Size: 118 8 65 101 343 218 545 59
Cuts at: 1457 1482
Size: 25
Fragments arranged by size:
545 343 218 118 101 65 59 25
8
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1144 1174 1482
Size: 1144 30 308
Fragments arranged by size:
1144 308 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 970 1438 1482
Size: 970 468 44
Fragments arranged by size:
970 468 44
BseYI C'CCAG_C
Cuts at: 0 703 753 858 934 1415 1429 1482
Size: 703 50 105 76 481 14 53
Fragments arranged by size:
703 481 105 76 53 50 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 247 613 1482
Size: 16 231 366 869
Fragments arranged by size:
869 366 231 16
BsiEI CG_ry'CG
Cuts at: 0 356 389 395 446 878 953 1116 1315
Size: 356 33 6 51 432 75 163 199
Cuts at: 1315 1482
Size: 167
Fragments arranged by size:
432 356 199 167 163 75 51 33
6
BsiHKAI G_wGCw'C
Cuts at: 0 1364 1482
Size: 1364 118
Bsp1286I G_dGCh'C
Cuts at: 0 73 259 1063 1364 1430 1450 1482
Size: 73 186 804 301 66 20 32
Fragments arranged by size:
804 301 186 73 66 32 20
BspHI T'CATG_A
Cuts at: 0 1385 1482
Size: 1385 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 347 1082 1277 1320 1482
Size: 347 735 195 43 162
Fragments arranged by size:
735 347 195 162 43
BsrBI CCG'CTC
Cuts at: 0 348 494 522 823 923 1482
Size: 348 146 28 301 100 559
Fragments arranged by size:
559 348 301 146 100 28
BsrDI GCAATG_nn'
Cuts at: 0 278 1482
Size: 278 1204
BsrFI r'CCGG_y
Cuts at: 0 142 1453 1482
Size: 142 1311 29
Fragments arranged by size:
1311 142 29
BssHII G'CGCG_C
Cuts at: 0 82 1227 1482
Size: 82 1145 255
Fragments arranged by size:
1145 255 82
BstAPI GCAn_nnn'nTGC
Cuts at: 0 284 339 1482
Size: 284 55 1143
Fragments arranged by size:
1143 284 55
BstEII G'GTnAC_C
Cuts at: 0 1332 1482
Size: 1332 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 703 975 1482
Size: 703 272 507
Fragments arranged by size:
703 507 272
BstYI r'GATC_y
Cuts at: 0 1040 1482
Size: 1040 442
BtgI C'CryG_G
Cuts at: 0 105 240 356 389 395 539 581 780
Size: 105 135 116 33 6 144 42 199
Cuts at: 780 839 1093 1460 1482
Size: 59 254 367 22
Fragments arranged by size:
367 254 199 144 135 116 105 59
42 33 22 6
DraIII CAC_nnn'GTG
Cuts at: 0 39 1482
Size: 39 1443
EaeI y'GGCC_r
Cuts at: 0 140 353 359 386 392 398 414 443
Size: 140 213 6 27 6 6 16 29
Cuts at: 443 482 674 875 950 1113 1312 1463 1482
Size: 39 192 201 75 163 199 151 19
Fragments arranged by size:
213 201 199 192 163 151 140 75
39 29 27 19 16 6 6 6
EagI C'GGCC_G
Cuts at: 0 353 386 392 443 875 950 1113 1312
Size: 353 33 6 51 432 75 163 199
Cuts at: 1312 1482
Size: 170
Fragments arranged by size:
432 353 199 170 163 75 51 33
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 833 1009 1482
Size: 833 176 473
Fragments arranged by size:
833 473 176
EcoICRI GAG'CTC
Cuts at: 0 1362 1482
Size: 1362 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1021 1482
Size: 34 987 461
Fragments arranged by size:
987 461 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1128 1482
Size: 1128 354
EcoO109I rG'GnC_Cy
Cuts at: 0 27 1446 1447 1482
Size: 27 1419 1 35
Fragments arranged by size:
1419 35 27 1
FspI TGC'GCA
Cuts at: 0 1081 1482
Size: 1081 401
HaeII r_GCGC'y
Cuts at: 0 121 129 194 295 532 638 839 856
Size: 121 8 65 101 237 106 201 17
Cuts at: 856 1008 1180 1254 1401 1460 1482
Size: 152 172 74 147 59 22
Fragments arranged by size:
237 201 172 152 147 121 106 101
74 65 59 22 17 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1032 1064 1482
Size: 1032 32 418
Fragments arranged by size:
1032 418 32
KasI G'GCGC_C
Cuts at: 0 117 125 190 291 634 852 1397 1456
Size: 117 8 65 101 343 218 545 59
Cuts at: 1456 1482
Size: 26
Fragments arranged by size:
545 343 218 117 101 65 59 26
8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1094 1482
Size: 1094 388
MslI CAynn'nnrTG
Cuts at: 0 508 1482
Size: 508 974
MspA1I CmG'CkG
Cuts at: 0 107 242 261 391 594 603 672 782
Size: 107 135 19 130 203 9 69 110
Cuts at: 782 815 830 841 858 949 1038 1403 1415
Size: 33 15 11 17 91 89 365 12
Cuts at: 1415 1482
Size: 67
Fragments arranged by size:
365 203 135 130 110 107 91 89
69 67 33 19 17 15 12 11
9
NaeI GCC'GGC
Cuts at: 0 144 1455 1482
Size: 144 1311 27
Fragments arranged by size:
1311 144 27
NarI GG'CG_CC
Cuts at: 0 118 126 191 292 635 853 1398 1457
Size: 118 8 65 101 343 218 545 59
Cuts at: 1457 1482
Size: 25
Fragments arranged by size:
545 343 218 118 101 65 59 25
8
NcoI C'CATG_G
Cuts at: 0 581 1482
Size: 581 901
NgoMIV G'CCGG_C
Cuts at: 0 142 1453 1482
Size: 142 1311 29
Fragments arranged by size:
1311 142 29
NotI GC'GGCC_GC
Cuts at: 0 386 443 1482
Size: 386 57 1039
Fragments arranged by size:
1039 386 57
NspI r_CATG'y
Cuts at: 0 1173 1482
Size: 1173 309
PflMI CCAn_nnn'nTGG
Cuts at: 0 1094 1482
Size: 1094 388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1144 1176 1482
Size: 1144 32 306
Fragments arranged by size:
1144 306 32
PspOMI G'GGCC_C
Cuts at: 0 255 1446 1482
Size: 255 1191 36
Fragments arranged by size:
1191 255 36
PstI C_TGCA'G
Cuts at: 0 335 1151 1238 1274 1482
Size: 335 816 87 36 208
Fragments arranged by size:
816 335 208 87 36
SacI G_AGCT'C
Cuts at: 0 1364 1482
Size: 1364 118
SacII CC_GC'GG
Cuts at: 0 108 243 392 783 842 1482
Size: 108 135 149 391 59 640
Fragments arranged by size:
640 391 149 135 108 59
SfcI C'TryA_G
Cuts at: 0 331 1008 1147 1234 1270 1482
Size: 331 677 139 87 36 212
Fragments arranged by size:
677 331 212 139 87 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 692 1482
Size: 692 790
SfoI GGC'GCC
Cuts at: 0 119 127 192 293 636 854 1399 1458
Size: 119 8 65 101 343 218 545 59
Cuts at: 1458 1482
Size: 24
Fragments arranged by size:
545 343 218 119 101 65 59 24
8
SgrAI Cr'CCGG_yG
Cuts at: 0 1453 1482
Size: 1453 29
SmaI CCC'GGG
Cuts at: 0 74 123 131 1443 1482
Size: 74 49 8 1312 39
Fragments arranged by size:
1312 74 49 39 8
SrfI GCCC'GGGC
Cuts at: 0 74 123 131 1482
Size: 74 49 8 1351
Fragments arranged by size:
1351 74 49 8
StyI C'CwwG_G
Cuts at: 0 581 968 1319 1475 1482
Size: 581 387 351 156 7
Fragments arranged by size:
581 387 351 156 7
XmaI C'CCGG_G
Cuts at: 0 72 121 129 1441 1482
Size: 72 49 8 1312 41
Fragments arranged by size:
1312 72 49 41 8
Enzymes that do cut:
AarI AcuI AfeI AlfI AloI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BglI BlpI
Bme1580I BpmI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssHII BstAPI BstEII BstXI BstYI BtgI
DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I KasI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PflMI PpiI PspOMI
PstI SacI SacII SfcI SfiI SfoI SgrAI SmaI
SrfI StyI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
BplI BpuEI BsaAI BsaWI BsiWI BsmI BsmBI BspEI
BsrGI BssSI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EarI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
NdeI NheI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI
SgfI SmlI SnaBI SpeI SphI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI