Restriction map for
pFN21AE1245
(Linear) (Six-base) MAPSORT of: KIEE1245.seq Check: 7594 from: 1 to: 1431
KIEE1245.orf
With 182 enzymes: SgfI *
March 22, 2012 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 750 1431
Size: 750 681
AleI CACnn'nnGTG
Cuts at: 0 352 1431
Size: 352 1079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 1431
Size: 202 1229
AlwNI CAG_nnn'CTG
Cuts at: 0 504 1167 1431
Size: 504 663 264
Fragments arranged by size:
663 504 264
ApaLI G'TGCA_C
Cuts at: 0 537 1431
Size: 537 894
AscI GG'CGCG_CC
Cuts at: 0 616 1431
Size: 616 815
AvaI C'yCGr_G
Cuts at: 0 148 1233 1270 1380 1431
Size: 148 1085 37 110 51
Fragments arranged by size:
1085 148 110 51 37
BanI G'GyrC_C
Cuts at: 0 25 99 333 1059 1199 1431
Size: 25 74 234 726 140 232
Fragments arranged by size:
726 234 232 140 74 25
BanII G_rGCy'C
Cuts at: 0 38 1340 1431
Size: 38 1302 91
Fragments arranged by size:
1302 91 38
BbeI G_GCGC'C
Cuts at: 0 29 1203 1431
Size: 29 1174 228
Fragments arranged by size:
1174 228 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 987 1071 1431
Size: 987 84 360
Fragments arranged by size:
987 360 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1154 1188 1431
Size: 1154 34 243
Fragments arranged by size:
1154 243 34
BglI GCCn_nnn'nGGC
Cuts at: 0 85 556 901 1108 1406 1431
Size: 85 471 345 207 298 25
Fragments arranged by size:
471 345 298 207 85 25
Bme1580I G_kGCm'C
Cuts at: 0 104 541 707 1064 1431
Size: 104 437 166 357 367
Fragments arranged by size:
437 367 357 166 104
BmgBI CAC'GTC
Cuts at: 0 414 1431
Size: 414 1017
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 396 1431
Size: 396 1035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1354 1431
Size: 1354 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 1431
Size: 952 479
BsaI GGTCTCn'nnnn_
Cuts at: 0 433 1211 1431
Size: 433 778 220
Fragments arranged by size:
778 433 220
BsaAI yAC'GTr
Cuts at: 0 702 1431
Size: 702 729
BsaHI Gr'CG_yC
Cuts at: 0 26 256 992 1200 1431
Size: 26 230 736 208 231
Fragments arranged by size:
736 231 230 208 26
BseYI C'CCAG_C
Cuts at: 0 368 804 975 1092 1431
Size: 368 436 171 117 339
Fragments arranged by size:
436 368 339 171 117
BsiEI CG_ry'CG
Cuts at: 0 56 814 1068 1225 1431
Size: 56 758 254 157 206
Fragments arranged by size:
758 254 206 157 56
BsiHKAI G_wGCw'C
Cuts at: 0 17 541 1431
Size: 17 524 890
Fragments arranged by size:
890 524 17
Bsp1286I G_dGCh'C
Cuts at: 0 17 38 104 541 707 1064 1340 1431
Size: 17 21 66 437 166 357 276 91
Fragments arranged by size:
437 357 276 166 91 66 21 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 740 750 1431
Size: 740 10 681
Fragments arranged by size:
740 681 10
BsrBI CCG'CTC
Cuts at: 0 63 1431
Size: 63 1368
BsrDI GCAATG_nn'
Cuts at: 0 231 452 1431
Size: 231 221 979
Fragments arranged by size:
979 231 221
BsrFI r'CCGG_y
Cuts at: 0 1196 1220 1345 1431
Size: 1196 24 125 86
Fragments arranged by size:
1196 125 86 24
BssHII G'CGCG_C
Cuts at: 0 513 515 616 1178 1180 1227 1324 1431
Size: 513 2 101 562 2 47 97 107
Fragments arranged by size:
562 513 107 101 97 47 2 2
BssSI C'ACGA_G
Cuts at: 0 532 940 1431
Size: 532 408 491
Fragments arranged by size:
532 491 408
BstEII G'GTnAC_C
Cuts at: 0 40 1431
Size: 40 1391
BstXI CCAn_nnnn'nTGG
Cuts at: 0 354 1431
Size: 354 1077
BtgI C'CryG_G
Cuts at: 0 83 259 1212 1431
Size: 83 176 953 219
Fragments arranged by size:
953 219 176 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 231 660 1250 1431
Size: 231 429 590 181
Fragments arranged by size:
590 429 231 181
BtsI GCAGTG_nn'
Cuts at: 0 172 1431
Size: 172 1259
DraIII CAC_nnn'GTG
Cuts at: 0 545 1431
Size: 545 886
DrdI GACnn_nn'nnGTC
Cuts at: 0 1300 1303 1431
Size: 1300 3 128
Fragments arranged by size:
1300 128 3
EaeI y'GGCC_r
Cuts at: 0 53 77 242 299 428 811 902 1025
Size: 53 24 165 57 129 383 91 123
Cuts at: 1025 1222 1431
Size: 197 209
Fragments arranged by size:
383 209 197 165 129 123 91 57
53 24
EagI C'GGCC_G
Cuts at: 0 53 811 1222 1431
Size: 53 758 411 209
Fragments arranged by size:
758 411 209 53
EarI CTCTTCn'nnn_
Cuts at: 0 289 1078 1431
Size: 289 789 353
Fragments arranged by size:
789 353 289
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 616 1384 1431
Size: 616 768 47
Fragments arranged by size:
768 616 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1354 1431
Size: 1354 77
EcoO109I rG'GnC_Cy
Cuts at: 0 528 1431
Size: 528 903
HaeII r_GCGC'y
Cuts at: 0 29 199 308 789 967 1203 1431
Size: 29 170 109 481 178 236 228
Fragments arranged by size:
481 236 228 178 170 109 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 1363 1395 1431
Size: 142 32 1189 32 36
Fragments arranged by size:
1189 142 36 32 32
KasI G'GCGC_C
Cuts at: 0 25 1199 1431
Size: 25 1174 232
Fragments arranged by size:
1174 232 25
MscI TGG'CCA
Cuts at: 0 244 301 904 1027 1431
Size: 244 57 603 123 404
Fragments arranged by size:
603 404 244 123 57
MslI CAynn'nnrTG
Cuts at: 0 352 355 583 985 1431
Size: 352 3 228 402 446
Fragments arranged by size:
446 402 352 228 3
MspA1I CmG'CkG
Cuts at: 0 85 156 175 261 368 623 779 900
Size: 85 71 19 86 107 255 156 121
Cuts at: 900 1431
Size: 531
Fragments arranged by size:
531 255 156 121 107 86 85 71
19
NaeI GCC'GGC
Cuts at: 0 1198 1347 1431
Size: 1198 149 84
Fragments arranged by size:
1198 149 84
NarI GG'CG_CC
Cuts at: 0 26 1200 1431
Size: 26 1174 231
Fragments arranged by size:
1174 231 26
NgoMIV G'CCGG_C
Cuts at: 0 1196 1345 1431
Size: 1196 149 86
Fragments arranged by size:
1196 149 86
NotI GC'GGCC_GC
Cuts at: 0 811 1431
Size: 811 620
PasI CC'CwG_GG
Cuts at: 0 1340 1431
Size: 1340 91
PspXI vC'TCGA_Gb
Cuts at: 0 1380 1431
Size: 1380 51
PstI C_TGCA'G
Cuts at: 0 269 1431
Size: 269 1162
PvuII CAG'CTG
Cuts at: 0 779 1431
Size: 779 652
SacII CC_GC'GG
Cuts at: 0 86 262 1431
Size: 86 176 1169
Fragments arranged by size:
1169 176 86
SexAI A'CCwGG_T
Cuts at: 0 311 1431
Size: 311 1120
SfcI C'TryA_G
Cuts at: 0 265 1431
Size: 265 1166
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 556 1431
Size: 556 875
SfoI GGC'GCC
Cuts at: 0 27 1201 1431
Size: 27 1174 230
Fragments arranged by size:
1174 230 27
SgrAI Cr'CCGG_yG
Cuts at: 0 1196 1431
Size: 1196 235
SmaI CCC'GGG
Cuts at: 0 1272 1431
Size: 1272 159
SmlI C'TyrA_G
Cuts at: 0 967 1380 1431
Size: 967 413 51
Fragments arranged by size:
967 413 51
StuI AGG'CCT
Cuts at: 0 1042 1156 1431
Size: 1042 114 275
Fragments arranged by size:
1042 275 114
StyI C'CwwG_G
Cuts at: 0 1424 1431
Size: 1424 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 403 435 1381 1413 1431
Size: 403 32 946 32 18
Fragments arranged by size:
946 403 32 32 18
Tth111I GACn'n_nGTC
Cuts at: 0 313 652 1300 1431
Size: 313 339 648 131
Fragments arranged by size:
648 339 313 131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 309 558 897 1431
Size: 309 249 339 534
Fragments arranged by size:
534 339 309 249
XhoI C'TCGA_G
Cuts at: 0 1380 1431
Size: 1380 51
XmaI C'CCGG_G
Cuts at: 0 1270 1431
Size: 1270 161
XmnI GAAnn'nnTTC
Cuts at: 0 1042 1431
Size: 1042 389
Enzymes that do cut:
AarI AleI AlfI AlwNI ApaLI AscI AvaI BanI
BanII BbeI BbsI BcgI BglI Bme1580I BmgBI BplI
BpmI BpuEI BsaI BsaAI BsaHI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstEII
BstXI BtgI BtgZI BtsI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI MscI
MslI MspA1I NaeI NarI NgoMIV NotI PasI PspXI
PstI PvuII SacII SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI StuI StyI TstI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApoI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI
BmrI BmtI Bpu10I BsaBI BsaWI BsaXI BseRI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BstAPI BstBI
BstYI BstZ17I Bsu36I ClaI CspCI DraI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SgfI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
XbaI ZraI