(Linear) (Six-base) MAPSORT of: KIEE1227.seq  Check: 1125  from: 1  to: 939



KIEE1227.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     481     939 
   Size:        481     458
 
ApoI r'AATT_y

Cuts at:      0     601     939 
   Size:        601     338
 
AvaI C'yCGr_G

Cuts at:      0     111     939 
   Size:        111     828
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     556     590     939 
   Size:        556      34     349

  Fragments arranged by size: 

                 556     349      34
 
BfrBI ATG'CAT

Cuts at:      0     435     667     939 
   Size:        435     232     272

  Fragments arranged by size: 

                 435     272     232
 
BglI GCCn_nnn'nGGC

Cuts at:      0      95     155     939 
   Size:         95      60     784

  Fragments arranged by size: 

                 784      95      60
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     728     748     939 
   Size:        728      20     191

  Fragments arranged by size: 

                 728     191      20
 
Bpu10I CC'TnA_GC

Cuts at:      0      41     939 
   Size:         41     898
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     380     416     939 
   Size:        380      36     523

  Fragments arranged by size: 

                 523     380      36
 
BsaWI w'CCGG_w

Cuts at:      0     407     939 
   Size:        407     532
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     112     142     939 
   Size:        112      30     797

  Fragments arranged by size: 

                 797     112      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     140     939 
   Size:        140     799
 
BsmI GAATG_Cn'

Cuts at:      0     667     939 
   Size:        667     272
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      94     939 
   Size:         94     845
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     939 
   Size:        140     799
 
BspHI T'CATG_A

Cuts at:      0     881     939 
   Size:        881      58
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     481     939 
   Size:        481     458
 
BsrBI CCG'CTC

Cuts at:      0     809     939 
   Size:        809     130
 
BsrDI GCAATG_nn'

Cuts at:      0     615     939 
   Size:        615     324
 
BsrGI T'GTAC_A

Cuts at:      0     176     939 
   Size:        176     763
 
BstYI r'GATC_y

Cuts at:      0     402     785     939 
   Size:        402     383     154

  Fragments arranged by size: 

                 402     383     154
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     728     748     939 
   Size:        728      20     191

  Fragments arranged by size: 

                 728     191      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0     426     939 
   Size:        426     513
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144     394     426     939 
   Size:        112      32     250      32     513

  Fragments arranged by size: 

                 513     250     112      32      32
 
HincII GTy'rAC

Cuts at:      0     498     939 
   Size:        498     441
 
HindIII A'AGCT_T

Cuts at:      0     514     939 
   Size:        514     425
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     809     929     939 
   Size:        809     120      10

  Fragments arranged by size: 

                 809     120      10
 
NsiI A_TGCA'T

Cuts at:      0     437     669     939 
   Size:        437     232     270

  Fragments arranged by size: 

                 437     270     232
 
PfoI T'CCnGG_A

Cuts at:      0     309     939 
   Size:        309     630
 
PstI C_TGCA'G

Cuts at:      0     443     492     939 
   Size:        443      49     447

  Fragments arranged by size: 

                 447     443      49
 
SfcI C'TryA_G

Cuts at:      0     439     488     939 
   Size:        439      49     451

  Fragments arranged by size: 

                 451     439      49
 
SmlI C'TyrA_G

Cuts at:      0     111     939 
   Size:        111     828
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      25     939 
   Size:         25     914
 
TatI w'GTAC_w

Cuts at:      0     176     939 
   Size:        176     763
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     348     796     939 
   Size:        348     448     143

  Fragments arranged by size: 

                 448     348     143
 
XhoI C'TCGA_G

Cuts at:      0     111     939 
   Size:        111     828
 

 Enzymes that do cut:

     AarI     ApoI     AvaI     BcgI    BfrBI     BglI     BpmI   Bpu10I
     BsaI    BsaWI    BsaXI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrGI    BstYI  Eco57MI EcoO109I    Hin4I
   HincII  HindIII     MmeI     NsiI     PfoI     PstI     SfcI     SmlI
    TaqII     TatI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI
    BseRI    BseYI     BsgI    BsiEI    BsiWI    BspEI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XmaI     XmnI     ZraI