Restriction map for
pFN21AE1225
(Linear) (Six-base) MAPSORT of: KIEE1225.seq Check: 1793 from: 1 to: 861
KIEE1225.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 527 861
Size: 356 171 334
Fragments arranged by size:
356 334 171
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 280 312 861
Size: 280 32 549
Fragments arranged by size:
549 280 32
BanI G'GyrC_C
Cuts at: 0 424 472 704 861
Size: 424 48 232 157
Fragments arranged by size:
424 232 157 48
BanII G_rGCy'C
Cuts at: 0 125 236 746 861
Size: 125 111 510 115
Fragments arranged by size:
510 125 115 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 344 861
Size: 344 517
BbvCI CC'TCA_GC
Cuts at: 0 199 861
Size: 199 662
BclI T'GATC_A
Cuts at: 0 786 815 861
Size: 786 29 46
Fragments arranged by size:
786 46 29
BglII A'GATC_T
Cuts at: 0 48 861
Size: 48 813
BlpI GC'TnA_GC
Cuts at: 0 5 861
Size: 5 856
Bme1580I G_kGCm'C
Cuts at: 0 427 475 840 861
Size: 427 48 365 21
Fragments arranged by size:
427 365 48 21
BmgBI CAC'GTC
Cuts at: 0 468 861
Size: 468 393
BmrI ACTGGGnnnn_n'
Cuts at: 0 465 627 835 861
Size: 465 162 208 26
Fragments arranged by size:
465 208 162 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 97 861
Size: 79 18 764
Fragments arranged by size:
764 79 18
Bpu10I CC'TnA_GC
Cuts at: 0 199 861
Size: 199 662
BsaI GGTCTCn'nnnn_
Cuts at: 0 168 861
Size: 168 693
BsaHI Gr'CG_yC
Cuts at: 0 750 861
Size: 750 111
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 360 605 861
Size: 360 245 256
Fragments arranged by size:
360 256 245
BsiEI CG_ry'CG
Cuts at: 0 447 861
Size: 447 414
BsiHKAI G_wGCw'C
Cuts at: 0 11 861
Size: 11 850
Bsp1286I G_dGCh'C
Cuts at: 0 11 125 236 427 475 746 840 861
Size: 11 114 111 191 48 271 94 21
Fragments arranged by size:
271 191 114 111 94 48 21 11
BsrDI GCAATG_nn'
Cuts at: 0 677 861
Size: 677 184
BstYI r'GATC_y
Cuts at: 0 48 861
Size: 48 813
Bsu36I CC'TnA_GG
Cuts at: 0 343 861
Size: 343 518
BtsI GCAGTG_nn'
Cuts at: 0 404 861
Size: 404 457
DraIII CAC_nnn'GTG
Cuts at: 0 645 861
Size: 645 216
EaeI y'GGCC_r
Cuts at: 0 444 861
Size: 444 417
EagI C'GGCC_G
Cuts at: 0 444 861
Size: 444 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 97 356 527 861
Size: 79 18 259 171 334
Fragments arranged by size:
334 259 171 79 18
HaeII r_GCGC'y
Cuts at: 0 208 861
Size: 208 653
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 861
Size: 20 32 809
Fragments arranged by size:
809 32 20
HincII GTy'rAC
Cuts at: 0 516 861
Size: 516 345
MslI CAynn'nnrTG
Cuts at: 0 770 861
Size: 770 91
MspA1I CmG'CkG
Cuts at: 0 829 861
Size: 829 32
PfoI T'CCnGG_A
Cuts at: 0 656 861
Size: 656 205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 792 824 861
Size: 792 32 37
Fragments arranged by size:
792 37 32
StyI C'CwwG_G
Cuts at: 0 265 861
Size: 265 596
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 612 692 861
Size: 612 80 169
Fragments arranged by size:
612 169 80
TatI w'GTAC_w
Cuts at: 0 191 861
Size: 191 670
Enzymes that do cut:
AcuI AloI BanI BanII BbsI BbvCI BclI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI
BseRI BsiEI BsiHKAI Bsp1286I BsrDI BstYI Bsu36I BtsI
DraIII EaeI EagI Eco57MI HaeII Hin4I HincII MslI
MspA1I PfoI PpiI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BcgI BciVI BfrBI BglI BmtI BplI BpuEI BsaAI
BsaBI BsaWI BsaXI BseYI BsgI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI
CspCI DraI DrdI EarI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI