Restriction map for
pFN21AE1224
(Linear) (Six-base) MAPSORT of: KIEE1224.seq Check: 9978 from: 1 to: 813
KIEE1224.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 680 813
Size: 466 214 133
Fragments arranged by size:
466 214 133
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 412 444 813
Size: 412 32 369
Fragments arranged by size:
412 369 32
AlwNI CAG_nnn'CTG
Cuts at: 0 538 731 813
Size: 538 193 82
Fragments arranged by size:
538 193 82
ApaI G_GGCC'C
Cuts at: 0 550 813
Size: 550 263
AvaI C'yCGr_G
Cuts at: 0 345 813
Size: 345 468
BanI G'GyrC_C
Cuts at: 0 406 568 738 813
Size: 406 162 170 75
Fragments arranged by size:
406 170 162 75
BanII G_rGCy'C
Cuts at: 0 550 608 813
Size: 550 58 205
Fragments arranged by size:
550 205 58
BglI GCCn_nnn'nGGC
Cuts at: 0 326 765 813
Size: 326 439 48
Fragments arranged by size:
439 326 48
Bme1580I G_kGCm'C
Cuts at: 0 409 550 743 813
Size: 409 141 193 70
Fragments arranged by size:
409 193 141 70
BmrI ACTGGGnnnn_n'
Cuts at: 0 220 813
Size: 220 593
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 597 813
Size: 597 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 622 669 708 813
Size: 453 169 47 39 105
Fragments arranged by size:
453 169 105 47 39
BsaAI yAC'GTr
Cuts at: 0 491 813
Size: 491 322
BseYI C'CCAG_C
Cuts at: 0 198 242 813
Size: 198 44 571
Fragments arranged by size:
571 198 44
BsmBI CGTCTCn'nnnn_
Cuts at: 0 647 813
Size: 647 166
Bsp1286I G_dGCh'C
Cuts at: 0 409 550 608 743 813
Size: 409 141 58 135 70
Fragments arranged by size:
409 141 135 70 58
BsrBI CCG'CTC
Cuts at: 0 645 813
Size: 645 168
BsrFI r'CCGG_y
Cuts at: 0 51 75 813
Size: 51 24 738
Fragments arranged by size:
738 51 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 719 813
Size: 719 94
BstEII G'GTnAC_C
Cuts at: 0 159 813
Size: 159 654
Bsu36I CC'TnA_GG
Cuts at: 0 11 813
Size: 11 802
BtsI GCAGTG_nn'
Cuts at: 0 113 813
Size: 113 700
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 669 704 813
Size: 669 35 109
Fragments arranged by size:
669 109 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 226 813
Size: 226 587
EaeI y'GGCC_r
Cuts at: 0 73 813
Size: 73 740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 466 622 669 680 708 813
Size: 453 13 156 47 11 28 105
Fragments arranged by size:
453 156 105 47 28 13 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 33 813
Size: 33 780
EcoO109I rG'GnC_Cy
Cuts at: 0 131 235 246 377 546 547 597 638
Size: 131 104 11 131 169 1 50 41
Cuts at: 638 668 813
Size: 30 145
Fragments arranged by size:
169 145 131 131 104 50 41 30
11 1
HaeII r_GCGC'y
Cuts at: 0 615 813
Size: 615 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 186 218 813
Size: 26 160 32 595
Fragments arranged by size:
595 160 32 26
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 607 813
Size: 42 565 206
Fragments arranged by size:
565 206 42
MspA1I CmG'CkG
Cuts at: 0 390 728 813
Size: 390 338 85
Fragments arranged by size:
390 338 85
NaeI GCC'GGC
Cuts at: 0 53 77 813
Size: 53 24 736
Fragments arranged by size:
736 53 24
NgoMIV G'CCGG_C
Cuts at: 0 51 75 813
Size: 51 24 738
Fragments arranged by size:
738 51 24
NspI r_CATG'y
Cuts at: 0 149 813
Size: 149 664
PasI CC'CwG_GG
Cuts at: 0 424 813
Size: 424 389
PflMI CCAn_nnn'nTGG
Cuts at: 0 731 813
Size: 731 82
PpuMI rG'GwC_Cy
Cuts at: 0 131 246 638 668 813
Size: 131 115 392 30 145
Fragments arranged by size:
392 145 131 115 30
PspOMI G'GGCC_C
Cuts at: 0 546 813
Size: 546 267
PstI C_TGCA'G
Cuts at: 0 341 479 508 534 555 813
Size: 341 138 29 26 21 258
Fragments arranged by size:
341 258 138 29 26 21
PvuII CAG'CTG
Cuts at: 0 390 728 813
Size: 390 338 85
Fragments arranged by size:
390 338 85
SanDI GG'GwC_CC
Cuts at: 0 246 668 813
Size: 246 422 145
Fragments arranged by size:
422 246 145
SfcI C'TryA_G
Cuts at: 0 337 475 504 530 551 813
Size: 337 138 29 26 21 262
Fragments arranged by size:
337 262 138 29 26 21
SmaI CCC'GGG
Cuts at: 0 347 813
Size: 347 466
SphI G_CATG'C
Cuts at: 0 149 813
Size: 149 664
StyI C'CwwG_G
Cuts at: 0 34 296 813
Size: 34 262 517
Fragments arranged by size:
517 262 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 110 147 575 813
Size: 110 37 428 238
Fragments arranged by size:
428 238 110 37
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 732 813
Size: 732 81
XmaI C'CCGG_G
Cuts at: 0 345 813
Size: 345 468
Enzymes that do cut:
AcuI AloI AlwNI ApaI AvaI BanI BanII BglI
Bme1580I BmrI BplI BpmI BsaAI BseYI BsmBI Bsp1286I
BsrBI BsrFI BstAPI BstEII Bsu36I BtsI CspCI DrdI
EaeI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MspA1I
NaeI NgoMIV NspI PasI PflMI PpuMI PspOMI PstI
PvuII SanDI SfcI SmaI SphI StyI TaqII XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrDI
BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I BtgI
BtgZI ClaI DraI DraIII EagI EarI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XhoI XmnI ZraI