Restriction map for
pFN21AE1216
(Linear) (Six-base) MAPSORT of: KIEE1216.seq Check: 8510 from: 1 to: 537
KIEE1216.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 537
Size: 493 44
AlwNI CAG_nnn'CTG
Cuts at: 0 199 537
Size: 199 338
ApaI G_GGCC'C
Cuts at: 0 220 537
Size: 220 317
AvaI C'yCGr_G
Cuts at: 0 74 537
Size: 74 463
BanI G'GyrC_C
Cuts at: 0 64 279 537
Size: 64 215 258
Fragments arranged by size:
258 215 64
BanII G_rGCy'C
Cuts at: 0 86 220 537
Size: 86 134 317
Fragments arranged by size:
317 134 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 481 537
Size: 83 398 56
Fragments arranged by size:
398 83 56
BbvCI CC'TCA_GC
Cuts at: 0 160 537
Size: 160 377
BciVI GTATCCnnnnn_n'
Cuts at: 0 37 537
Size: 37 500
BglI GCCn_nnn'nGGC
Cuts at: 0 224 537
Size: 224 313
Bme1580I G_kGCm'C
Cuts at: 0 220 537
Size: 220 317
BmrI ACTGGGnnnn_n'
Cuts at: 0 222 537
Size: 222 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 537
Size: 151 386
Bpu10I CC'TnA_GC
Cuts at: 0 160 427 537
Size: 160 267 110
Fragments arranged by size:
267 160 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 537
Size: 13 524
BsaWI w'CCGG_w
Cuts at: 0 86 537
Size: 86 451
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 537
Size: 148 389
BseYI C'CCAG_C
Cuts at: 0 503 537
Size: 503 34
Bsp1286I G_dGCh'C
Cuts at: 0 86 220 537
Size: 86 134 317
Fragments arranged by size:
317 134 86
BsrBI CCG'CTC
Cuts at: 0 395 537
Size: 395 142
BsrDI GCAATG_nn'
Cuts at: 0 305 537
Size: 305 232
BssSI C'ACGA_G
Cuts at: 0 5 537
Size: 5 532
BstYI r'GATC_y
Cuts at: 0 452 537
Size: 452 85
BtgI C'CryG_G
Cuts at: 0 101 228 537
Size: 101 127 309
Fragments arranged by size:
309 127 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 500 537
Size: 500 37
DraIII CAC_nnn'GTG
Cuts at: 0 279 537
Size: 279 258
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 537
Size: 106 431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 493 537
Size: 151 342 44
Fragments arranged by size:
342 151 44
EcoO109I rG'GnC_Cy
Cuts at: 0 216 217 369 537
Size: 216 1 152 168
Fragments arranged by size:
216 168 152 1
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 537
Size: 364 173
MspA1I CmG'CkG
Cuts at: 0 471 537
Size: 471 66
NcoI C'CATG_G
Cuts at: 0 228 537
Size: 228 309
NspI r_CATG'y
Cuts at: 0 188 537
Size: 188 349
PpuMI rG'GwC_Cy
Cuts at: 0 369 537
Size: 369 168
PspOMI G'GGCC_C
Cuts at: 0 216 537
Size: 216 321
PstI C_TGCA'G
Cuts at: 0 225 537
Size: 225 312
SbfI CC_TGCA'GG
Cuts at: 0 225 537
Size: 225 312
SexAI A'CCwGG_T
Cuts at: 0 275 537
Size: 275 262
SfcI C'TryA_G
Cuts at: 0 221 537
Size: 221 316
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 224 537
Size: 224 313
SmaI CCC'GGG
Cuts at: 0 76 537
Size: 76 461
SmlI C'TyrA_G
Cuts at: 0 28 537
Size: 28 509
SphI G_CATG'C
Cuts at: 0 188 537
Size: 188 349
StyI C'CwwG_G
Cuts at: 0 228 488 531 537
Size: 228 260 43 6
Fragments arranged by size:
260 228 43 6
XmaI C'CCGG_G
Cuts at: 0 74 537
Size: 74 463
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanI BanII BbsI BbvCI
BciVI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaWI
BseRI BseYI Bsp1286I BsrBI BsrDI BssSI BstYI BtgI
BtgZI DraIII EciI Eco57MI EcoO109I MmeI MspA1I NcoI
NspI PpuMI PspOMI PstI SbfI SexAI SfcI SfiI
SmaI SmlI SphI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BsaI BsaAI
BsaBI BsaHI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI ScaI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI