(Linear) (Six-base) MAPSORT of: KIEE1214.seq  Check: 1887  from: 1  to: 408



KIEE1214.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:35  ..

AccI GT'mk_AC

Cuts at:      0     172     408 
   Size:        172     236
 
AfeI AGC'GCT

Cuts at:      0      87     408 
   Size:         87     321
 
AflIII A'CryG_T

Cuts at:      0     348     408 
   Size:        348      60
 
AlwNI CAG_nnn'CTG

Cuts at:      0     210     408 
   Size:        210     198
 
BanI G'GyrC_C

Cuts at:      0     133     408 
   Size:        133     275
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       4      38     408 
   Size:          4      34     370

  Fragments arranged by size: 

                 370      34       4
 
BglI GCCn_nnn'nGGC

Cuts at:      0      70     292     408 
   Size:         70     222     116

  Fragments arranged by size: 

                 222     116      70
 
Bpu10I CC'TnA_GC

Cuts at:      0     227     408 
   Size:        227     181
 
BsaWI w'CCGG_w

Cuts at:      0     188     408 
   Size:        188     220
 
BsmI GAATG_Cn'

Cuts at:      0     389     408 
   Size:        389      19
 
BspEI T'CCGG_A

Cuts at:      0     188     408 
   Size:        188     220
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     254     408 
   Size:        254     154
 
BstYI r'GATC_y

Cuts at:      0     152     408 
   Size:        152     256
 
BtgI C'CryG_G

Cuts at:      0      95     137     263     408 
   Size:         95      42     126     145

  Fragments arranged by size: 

                 145     126      95      42
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     103     408 
   Size:        103     305
 
EaeI y'GGCC_r

Cuts at:      0      62      92     408 
   Size:         62      30     316

  Fragments arranged by size: 

                 316      62      30
 
HaeII r_GCGC'y

Cuts at:      0      89     210     277     408 
   Size:         89     121      67     131

  Fragments arranged by size: 

                 131     121      89      67
 
MluI A'CGCG_T

Cuts at:      0     348     408 
   Size:        348      60
 
MscI TGG'CCA

Cuts at:      0      64     408 
   Size:         64     344
 
MspA1I CmG'CkG

Cuts at:      0      60     265     408 
   Size:         60     205     143

  Fragments arranged by size: 

                 205     143      60
 
PstI C_TGCA'G

Cuts at:      0     281     408 
   Size:        281     127
 
PvuII CAG'CTG

Cuts at:      0      60     408 
   Size:         60     348
 
SacII CC_GC'GG

Cuts at:      0     266     408 
   Size:        266     142
 
SfcI C'TryA_G

Cuts at:      0     277     408 
   Size:        277     131
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     292     408 
   Size:        292     116
 
StuI AGG'CCT

Cuts at:      0     286     295     408 
   Size:        286       9     113

  Fragments arranged by size: 

                 286     113       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     256     408 
   Size:        256     152
 

 Enzymes that do cut:

     AccI     AfeI   AflIII    AlwNI     BanI     BcgI     BglI   Bpu10I
    BsaWI     BsmI    BspEI    BspMI    BstYI     BtgI    BtgZI     EaeI
    HaeII     MluI     MscI   MspA1I     PstI    PvuII    SacII     SfcI
     SfiI     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI