(Linear) (Six-base) MAPSORT of: KIEE1213.seq  Check: 2812  from: 1  to: 408



KIEE1213.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:35  ..

AflIII A'CryG_T

Cuts at:      0     167     408 
   Size:        167     241
 
ApoI r'AATT_y

Cuts at:      0      62     408 
   Size:         62     346
 
AvaI C'yCGr_G

Cuts at:      0      31     408 
   Size:         31     377
 
BanII G_rGCy'C

Cuts at:      0     133     408 
   Size:        133     275
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     174     408 
   Size:        174     234
 
BmgBI CAC'GTC

Cuts at:      0     170     381     408 
   Size:        170     211      27

  Fragments arranged by size: 

                 211     170      27
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     309     408 
   Size:        309      99
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     147     219     408 
   Size:        147      72     189

  Fragments arranged by size: 

                 189     147      72
 
BsaHI Gr'CG_yC

Cuts at:      0     311     408 
   Size:        311      97
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     136     232     408 
   Size:        136      96     176

  Fragments arranged by size: 

                 176     136      96
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     408 
   Size:        403       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     269     408 
   Size:        269     139
 
Bsp1286I G_dGCh'C

Cuts at:      0     133     269     408 
   Size:        133     136     139

  Fragments arranged by size: 

                 139     136     133
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      83     116     408 
   Size:         83      33     292

  Fragments arranged by size: 

                 292      83      33
 
BssHII G'CGCG_C

Cuts at:      0     349     408 
   Size:        349      59
 
BstYI r'GATC_y

Cuts at:      0     401     408 
   Size:        401       7
 
BtgI C'CryG_G

Cuts at:      0     299     408 
   Size:        299     109
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     258     408 
   Size:        258     150
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      31     319     408 
   Size:         31     288      89

  Fragments arranged by size: 

                 288      89      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     364     408 
   Size:        364      44
 
EcoRI G'AATT_C

Cuts at:      0      62     408 
   Size:         62     346
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     152     408 
   Size:         29     123     256

  Fragments arranged by size: 

                 256     123      29
 
MspA1I CmG'CkG

Cuts at:      0     345     387     408 
   Size:        345      42      21

  Fragments arranged by size: 

                 345      42      21
 
PvuII CAG'CTG

Cuts at:      0     387     408 
   Size:        387      21
 

 Enzymes that do cut:

   AflIII     ApoI     AvaI    BanII     BbsI    BmgBI     BmrI     BplI
    BsaHI    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI   BssHII    BstYI
     BtgI    BtgZI     EciI    EcoNI    EcoRI     MmeI   MspA1I    PvuII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI