(Linear) (Six-base) MAPSORT of: KIEE1211.seq  Check: 1808  from: 1  to: 396



KIEE1211.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:34  ..

ApoI r'AATT_y

Cuts at:      0      39     360     377     396 
   Size:         39     321      17      19

  Fragments arranged by size: 

                 321      39      19      17
 
BanII G_rGCy'C

Cuts at:      0       7     396 
   Size:          7     389
 
BbvCI CC'TCA_GC

Cuts at:      0      45     396 
   Size:         45     351
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     223     396 
   Size:        223     173
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     312     396 
   Size:        312      84
 
Bpu10I CC'TnA_GC

Cuts at:      0      45     396 
   Size:         45     351
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     396 
   Size:        269     127
 
BsiHKAI G_wGCw'C

Cuts at:      0     208     396 
   Size:        208     188
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     208     396 
   Size:          7     201     188

  Fragments arranged by size: 

                 201     188       7
 
BtsI GCAGTG_nn'

Cuts at:      0     252     396 
   Size:        252     144
 
DraI TTT'AAA

Cuts at:      0      71     396 
   Size:         71     325
 
EarI CTCTTCn'nnn_

Cuts at:      0      25     396 
   Size:         25     371
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     281     313     396 
   Size:        281      32      83

  Fragments arranged by size: 

                 281      83      32
 
StyI C'CwwG_G

Cuts at:      0     185     396 
   Size:        185     211
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     389     396 
   Size:        389       7
 
TatI w'GTAC_w

Cuts at:      0     310     396 
   Size:        310      86
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     375     396 
   Size:        375      21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     154     396 
   Size:        154     242
 

 Enzymes that do cut:

     ApoI    BanII    BbvCI    BciVI     BmrI   Bpu10I     BsgI  BsiHKAI
 Bsp1286I     BtsI     DraI     EarI    Hin4I     StyI    TaqII     TatI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI