(Linear) (Six-base) MAPSORT of: KIEE1205.seq  Check: 6405  from: 1  to: 378



KIEE1205.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:34  ..

AccI GT'mk_AC

Cuts at:      0     364     378 
   Size:        364      14
 
Acc65I G'GTAC_C

Cuts at:      0     343     378 
   Size:        343      35
 
AfeI AGC'GCT

Cuts at:      0     259     378 
   Size:        259     119
 
AlwNI CAG_nnn'CTG

Cuts at:      0      25     378 
   Size:         25     353
 
ApoI r'AATT_y

Cuts at:      0     189     378 
   Size:        189     189
 
BanI G'GyrC_C

Cuts at:      0     343     378 
   Size:        343      35
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     129     378 
   Size:        129     249
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     378 
   Size:        258     120
 
BspHI T'CATG_A

Cuts at:      0     185     378 
   Size:        185     193
 
BsrGI T'GTAC_A

Cuts at:      0     125     378 
   Size:        125     253
 
BstBI TT'CG_AA

Cuts at:      0     212     378 
   Size:        212     166
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     177     378 
   Size:        177     201
 
BstYI r'GATC_y

Cuts at:      0     281     378 
   Size:        281      97
 
BstZ17I GTA'TAC

Cuts at:      0     365     378 
   Size:        365      13
 
BtgI C'CryG_G

Cuts at:      0     176     378 
   Size:        176     202
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     204     378 
   Size:        204     174
 
BtsI GCAGTG_nn'

Cuts at:      0     338     378 
   Size:        338      40
 
DraIII CAC_nnn'GTG

Cuts at:      0     333     378 
   Size:        333      45
 
EaeI y'GGCC_r

Cuts at:      0     320     378 
   Size:        320      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     378 
   Size:        258     120
 
EcoRI G'AATT_C

Cuts at:      0     189     378 
   Size:        189     189
 
HaeII r_GCGC'y

Cuts at:      0     261     378 
   Size:        261     117
 
HincII GTy'rAC

Cuts at:      0     201     378 
   Size:        201     177
 
HpaI GTT'AAC

Cuts at:      0     201     378 
   Size:        201     177
 
KpnI G_GTAC'C

Cuts at:      0     347     378 
   Size:        347      31
 
MscI TGG'CCA

Cuts at:      0     322     378 
   Size:        322      56
 
NcoI C'CATG_G

Cuts at:      0     176     378 
   Size:        176     202
 
PfoI T'CCnGG_A

Cuts at:      0     309     378 
   Size:        309      69
 
StyI C'CwwG_G

Cuts at:      0      59     170     176     347     378 
   Size:         59     111       6     171      31

  Fragments arranged by size: 

                 171     111      59      31       6
 
TatI w'GTAC_w

Cuts at:      0     125     378 
   Size:        125     253
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     319     351     378 
   Size:        319      32      27

  Fragments arranged by size: 

                 319      32      27
 
XmnI GAAnn'nnTTC

Cuts at:      0     193     378 
   Size:        193     185
 

 Enzymes that do cut:

     AccI   Acc65I     AfeI    AlwNI     ApoI     BanI     BplI     BpmI
    BspHI    BsrGI    BstBI    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI   DraIII     EaeI  Eco57MI    EcoRI    HaeII   HincII     HpaI
     KpnI     MscI     NcoI     PfoI     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI   BstEII   Bsu36I     ClaI    CspCI
     DraI     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII     KasI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI