Restriction map for
pFN21AE1199
(Linear) (Six-base) MAPSORT of: KIEE1199.seq Check: 9241 from: 1 to: 1104
KIEE1199.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 42 1104
Size: 42 1062
AccI GT'mk_AC
Cuts at: 0 428 1104
Size: 428 676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 283 1104
Size: 220 63 821
Fragments arranged by size:
821 220 63
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 691 723 1104
Size: 691 32 381
Fragments arranged by size:
691 381 32
AlwNI CAG_nnn'CTG
Cuts at: 0 403 1104
Size: 403 701
ApaI G_GGCC'C
Cuts at: 0 536 653 857 988 1064 1104
Size: 536 117 204 131 76 40
Fragments arranged by size:
536 204 131 117 76 40
ApaLI G'TGCA_C
Cuts at: 0 547 1104
Size: 547 557
AvaI C'yCGr_G
Cuts at: 0 253 653 840 979 1104
Size: 253 400 187 139 125
Fragments arranged by size:
400 253 187 139 125
BanI G'GyrC_C
Cuts at: 0 922 1104
Size: 922 182
BanII G_rGCy'C
Cuts at: 0 224 536 653 857 988 1064 1104
Size: 224 312 117 204 131 76 40
Fragments arranged by size:
312 224 204 131 117 76 40
BbeI G_GCGC'C
Cuts at: 0 926 1104
Size: 926 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 264 298 1104
Size: 264 34 806
Fragments arranged by size:
806 264 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 401 1104
Size: 401 703
BglI GCCn_nnn'nGGC
Cuts at: 0 1053 1104
Size: 1053 51
BglII A'GATC_T
Cuts at: 0 20 1104
Size: 20 1084
BlpI GC'TnA_GC
Cuts at: 0 103 1104
Size: 103 1001
Bme1580I G_kGCm'C
Cuts at: 0 536 551 653 857 988 1064 1104
Size: 536 15 102 204 131 76 40
Fragments arranged by size:
536 204 131 102 76 40 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 455 742 872 937 1104
Size: 455 287 130 65 167
Fragments arranged by size:
455 287 167 130 65
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 984 1104
Size: 984 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 657 1104
Size: 319 338 447
Fragments arranged by size:
447 338 319
BsaI GGTCTCn'nnnn_
Cuts at: 0 776 1104
Size: 776 328
BsaHI Gr'CG_yC
Cuts at: 0 923 1104
Size: 923 181
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 691 702 721 732 1104
Size: 691 11 19 11 372
Fragments arranged by size:
691 372 19 11 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 916 1015 1104
Size: 334 582 99 89
Fragments arranged by size:
582 334 99 89
BseYI C'CCAG_C
Cuts at: 0 412 646 872 1104
Size: 412 234 226 232
Fragments arranged by size:
412 234 232 226
BsiHKAI G_wGCw'C
Cuts at: 0 113 551 1104
Size: 113 438 553
Fragments arranged by size:
553 438 113
BsmBI CGTCTCn'nnnn_
Cuts at: 0 250 1104
Size: 250 854
Bsp1286I G_dGCh'C
Cuts at: 0 113 224 536 551 653 857 988 1064
Size: 113 111 312 15 102 204 131 76
Cuts at: 1064 1104
Size: 40
Fragments arranged by size:
312 204 131 113 111 102 76 40
15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 42 611 638 1104
Size: 42 569 27 466
Fragments arranged by size:
569 466 42 27
BsrFI r'CCGG_y
Cuts at: 0 1073 1104
Size: 1073 31
BssSI C'ACGA_G
Cuts at: 0 226 1104
Size: 226 878
BstAPI GCAn_nnn'nTGC
Cuts at: 0 403 1104
Size: 403 701
BstXI CCAn_nnnn'nTGG
Cuts at: 0 880 1104
Size: 880 224
BstYI r'GATC_y
Cuts at: 0 20 1104
Size: 20 1084
BstZ17I GTA'TAC
Cuts at: 0 429 1104
Size: 429 675
Bsu36I CC'TnA_GG
Cuts at: 0 317 1104
Size: 317 787
BtgI C'CryG_G
Cuts at: 0 918 1104
Size: 918 186
BtsI GCAGTG_nn'
Cuts at: 0 478 710 1104
Size: 478 232 394
Fragments arranged by size:
478 394 232
EaeI y'GGCC_r
Cuts at: 0 1085 1104
Size: 1085 19
EarI CTCTTCn'nnn_
Cuts at: 0 166 562 1104
Size: 166 396 542
Fragments arranged by size:
542 396 166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 633 1104
Size: 633 471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 283 319 657 1104
Size: 220 63 36 338 447
Fragments arranged by size:
447 338 220 63 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 898 1104
Size: 898 206
EcoO109I rG'GnC_Cy
Cuts at: 0 313 434 533 650 760 854 907 984
Size: 313 121 99 117 110 94 53 77
Cuts at: 984 985 1104
Size: 1 119
Fragments arranged by size:
313 121 119 117 110 99 94 77
53 1
HaeII r_GCGC'y
Cuts at: 0 152 926 966 1104
Size: 152 774 40 138
Fragments arranged by size:
774 152 138 40
KasI G'GCGC_C
Cuts at: 0 922 1104
Size: 922 182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 1104
Size: 534 570
MslI CAynn'nnrTG
Cuts at: 0 181 1104
Size: 181 923
MspA1I CmG'CkG
Cuts at: 0 920 1104
Size: 920 184
NaeI GCC'GGC
Cuts at: 0 1075 1104
Size: 1075 29
NarI GG'CG_CC
Cuts at: 0 923 1104
Size: 923 181
NgoMIV G'CCGG_C
Cuts at: 0 1073 1104
Size: 1073 31
PflMI CCAn_nnn'nTGG
Cuts at: 0 772 1104
Size: 772 332
PfoI T'CCnGG_A
Cuts at: 0 32 720 1104
Size: 32 688 384
Fragments arranged by size:
688 384 32
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 1104
Size: 691 32 381
Fragments arranged by size:
691 381 32
PpuMI rG'GwC_Cy
Cuts at: 0 907 1104
Size: 907 197
PspOMI G'GGCC_C
Cuts at: 0 532 649 853 984 1060 1104
Size: 532 117 204 131 76 44
Fragments arranged by size:
532 204 131 117 76 44
PspXI vC'TCGA_Gb
Cuts at: 0 840 1104
Size: 840 264
PstI C_TGCA'G
Cuts at: 0 399 698 1104
Size: 399 299 406
Fragments arranged by size:
406 399 299
SacII CC_GC'GG
Cuts at: 0 921 1104
Size: 921 183
SanDI GG'GwC_CC
Cuts at: 0 907 1104
Size: 907 197
SfcI C'TryA_G
Cuts at: 0 395 684 694 1104
Size: 395 289 10 410
Fragments arranged by size:
410 395 289 10
SfoI GGC'GCC
Cuts at: 0 924 1104
Size: 924 180
SmaI CCC'GGG
Cuts at: 0 981 1104
Size: 981 123
SmlI C'TyrA_G
Cuts at: 0 253 840 1104
Size: 253 587 264
Fragments arranged by size:
587 264 253
StuI AGG'CCT
Cuts at: 0 94 542 1104
Size: 94 448 562
Fragments arranged by size:
562 448 94
StyI C'CwwG_G
Cuts at: 0 1082 1104
Size: 1082 22
TatI w'GTAC_w
Cuts at: 0 203 212 1104
Size: 203 9 892
Fragments arranged by size:
892 203 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 702 734 1104
Size: 702 32 370
Fragments arranged by size:
702 370 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 420 1104
Size: 180 240 684
Fragments arranged by size:
684 240 180
XhoI C'TCGA_G
Cuts at: 0 253 840 1104
Size: 253 587 264
Fragments arranged by size:
587 264 253
XmaI C'CCGG_G
Cuts at: 0 979 1104
Size: 979 125
Enzymes that do cut:
AarI AccI AcuI AloI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BcgI BciVI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BsaI BsaHI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtsI EaeI EarI
EciI Eco57MI EcoNI EcoO109I HaeII KasI MmeI MslI
MspA1I NaeI NarI NgoMIV PflMI PfoI PpiI PpuMI
PspOMI PspXI PstI SacII SanDI SfcI SfoI SmaI
SmlI StuI StyI TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BclI BfrBI BmgBI BmtI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PshAI PsiI PsrI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII
Tth111I XbaI XmnI ZraI