Restriction map for
pFN21AE1183
(Linear) (Six-base) MAPSORT of: KIEE1183.seq Check: 2039 from: 1 to: 2109
KIEE1183.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2079 2109
Size: 2079 30
AccI GT'mk_AC
Cuts at: 0 35 455 2109
Size: 35 420 1654
Fragments arranged by size:
1654 420 35
Acc65I G'GTAC_C
Cuts at: 0 515 2109
Size: 515 1594
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1055 1597 2109
Size: 1055 542 512
Fragments arranged by size:
1055 542 512
AflIII A'CryG_T
Cuts at: 0 11 983 1931 2109
Size: 11 972 948 178
Fragments arranged by size:
972 948 178 11
AgeI A'CCGG_T
Cuts at: 0 512 2109
Size: 512 1597
AleI CACnn'nnGTG
Cuts at: 0 1855 2109
Size: 1855 254
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1918 1950 2109
Size: 1918 32 159
Fragments arranged by size:
1918 159 32
AlwNI CAG_nnn'CTG
Cuts at: 0 639 2049 2109
Size: 639 1410 60
Fragments arranged by size:
1410 639 60
ApaI G_GGCC'C
Cuts at: 0 678 833 2109
Size: 678 155 1276
Fragments arranged by size:
1276 678 155
ApaLI G'TGCA_C
Cuts at: 0 1533 2109
Size: 1533 576
AvaI C'yCGr_G
Cuts at: 0 286 737 2109
Size: 286 451 1372
Fragments arranged by size:
1372 451 286
BanI G'GyrC_C
Cuts at: 0 515 661 816 1143 1448 1490 2109
Size: 515 146 155 327 305 42 619
Fragments arranged by size:
619 515 327 305 155 146 42
BanII G_rGCy'C
Cuts at: 0 89 678 833 1159 1381 1567 1879 2093
Size: 89 589 155 326 222 186 312 214
Cuts at: 2093 2109
Size: 16
Fragments arranged by size:
589 326 312 222 214 186 155 89
16
BbeI G_GCGC'C
Cuts at: 0 1147 1452 2109
Size: 1147 305 657
Fragments arranged by size:
1147 657 305
BbsI GAAGACnn'nnnn_
Cuts at: 0 478 997 1402 2109
Size: 478 519 405 707
Fragments arranged by size:
707 519 478 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 147 181 2109
Size: 147 34 1928
Fragments arranged by size:
1928 147 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 759 2109
Size: 759 1350
BglI GCCn_nnn'nGGC
Cuts at: 0 808 1525 1702 2058 2109
Size: 808 717 177 356 51
Fragments arranged by size:
808 717 356 177 51
BlpI GC'TnA_GC
Cuts at: 0 1678 2109
Size: 1678 431
Bme1580I G_kGCm'C
Cuts at: 0 664 678 833 1537 2109
Size: 664 14 155 704 572
Fragments arranged by size:
704 664 572 155 14
BmgBI CAC'GTC
Cuts at: 0 1932 2109
Size: 1932 177
BmrI ACTGGGnnnn_n'
Cuts at: 0 1882 2109
Size: 1882 227
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 66 2109
Size: 66 2043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 138 361 442 2109
Size: 36 102 223 81 1667
Fragments arranged by size:
1667 223 102 81 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1642 2109
Size: 394 1248 467
Fragments arranged by size:
1248 467 394
BsaI GGTCTCn'nnnn_
Cuts at: 0 391 560 577 1618 1948 2109
Size: 391 169 17 1041 330 161
Fragments arranged by size:
1041 391 330 169 161 17
BsaAI yAC'GTr
Cuts at: 0 1008 2109
Size: 1008 1101
BsaHI Gr'CG_yC
Cuts at: 0 1144 1449 1690 1919 2109
Size: 1144 305 241 229 190
Fragments arranged by size:
1144 305 241 229 190
BsaWI w'CCGG_w
Cuts at: 0 71 137 267 512 2109
Size: 71 66 130 245 1597
Fragments arranged by size:
1597 245 130 71 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 17 47 778 808 847 877 1708 1738
Size: 17 30 731 30 39 30 831 30
Cuts at: 1738 2109
Size: 371
Fragments arranged by size:
831 731 371 39 30 30 30 30
17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 97 568 871 1966 2109
Size: 39 58 471 303 1095 143
Fragments arranged by size:
1095 471 303 143 58 39
BseYI C'CCAG_C
Cuts at: 0 706 1359 2109
Size: 706 653 750
Fragments arranged by size:
750 706 653
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 697 1030 1402 2109
Size: 396 301 333 372 707
Fragments arranged by size:
707 396 372 333 301
BsiHKAI G_wGCw'C
Cuts at: 0 89 839 1537 1679 2109
Size: 89 750 698 142 430
Fragments arranged by size:
750 698 430 142 89
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1249 2109
Size: 1249 860
Bsp1286I G_dGCh'C
Cuts at: 0 89 664 678 833 839 1159 1381 1537
Size: 89 575 14 155 6 320 222 156
Cuts at: 1537 1567 1679 1879 2093 2109
Size: 30 112 200 214 16
Fragments arranged by size:
575 320 222 214 200 156 155 112
89 30 16 14 6
BspEI T'CCGG_A
Cuts at: 0 71 267 2109
Size: 71 196 1842
Fragments arranged by size:
1842 196 71
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 290 1508 2079 2109
Size: 290 1218 571 30
Fragments arranged by size:
1218 571 290 30
BsrBI CCG'CTC
Cuts at: 0 426 653 2109
Size: 426 227 1456
Fragments arranged by size:
1456 426 227
BsrFI r'CCGG_y
Cuts at: 0 512 1120 2009 2109
Size: 512 608 889 100
Fragments arranged by size:
889 608 512 100
BsrGI T'GTAC_A
Cuts at: 0 14 2109
Size: 14 2095
BstAPI GCAn_nnn'nTGC
Cuts at: 0 306 2109
Size: 306 1803
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1159 1737 1806 2109
Size: 1159 578 69 303
Fragments arranged by size:
1159 578 303 69
BstYI r'GATC_y
Cuts at: 0 1463 1964 2109
Size: 1463 501 145
Fragments arranged by size:
1463 501 145
BtgI C'CryG_G
Cuts at: 0 761 946 1350 1474 1788 2056 2109
Size: 761 185 404 124 314 268 53
Fragments arranged by size:
761 404 314 268 185 124 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 150 2010 2109
Size: 150 1860 99
Fragments arranged by size:
1860 150 99
BtsI GCAGTG_nn'
Cuts at: 0 1243 2109
Size: 1243 866
EaeI y'GGCC_r
Cuts at: 0 2 98 173 561 1167 1517 1943 2059
Size: 2 96 75 388 606 350 426 116
Cuts at: 2059 2109
Size: 50
Fragments arranged by size:
606 426 388 350 116 96 75 50
2
EarI CTCTTCn'nnn_
Cuts at: 0 245 2109
Size: 245 1864
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 238 1917 2109
Size: 238 1679 192
Fragments arranged by size:
1679 238 192
EcoICRI GAG'CTC
Cuts at: 0 87 2109
Size: 87 2022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 138 361 442 1055 1597 2109
Size: 36 102 223 81 613 542 512
Fragments arranged by size:
613 542 512 223 102 81 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 886 2109
Size: 886 1223
EcoO109I rG'GnC_Cy
Cuts at: 0 675 1222 2046 2109
Size: 675 547 824 63
Fragments arranged by size:
824 675 547 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1627 2109
Size: 1627 482
FspI TGC'GCA
Cuts at: 0 1251 2109
Size: 1251 858
HaeII r_GCGC'y
Cuts at: 0 725 814 1147 1452 2109
Size: 725 89 333 305 657
Fragments arranged by size:
725 657 333 305 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 388 420 847 879 2109
Size: 17 32 339 32 427 32 1230
Fragments arranged by size:
1230 427 339 32 32 32 17
HincII GTy'rAC
Cuts at: 0 1107 2082 2109
Size: 1107 975 27
Fragments arranged by size:
1107 975 27
KasI G'GCGC_C
Cuts at: 0 1143 1448 2109
Size: 1143 305 661
Fragments arranged by size:
1143 661 305
KpnI G_GTAC'C
Cuts at: 0 519 2109
Size: 519 1590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 560 723 1240 2109
Size: 3 557 163 517 869
Fragments arranged by size:
869 557 517 163 3
MscI TGG'CCA
Cuts at: 0 1945 2109
Size: 1945 164
MslI CAynn'nnrTG
Cuts at: 0 1855 2109
Size: 1855 254
MspA1I CmG'CkG
Cuts at: 0 306 339 730 1033 1373 1569 1599 2058
Size: 306 33 391 303 340 196 30 459
Cuts at: 2058 2109
Size: 51
Fragments arranged by size:
459 391 340 306 303 196 51 33
30
NaeI GCC'GGC
Cuts at: 0 2011 2109
Size: 2011 98
NarI GG'CG_CC
Cuts at: 0 1144 1449 2109
Size: 1144 305 660
Fragments arranged by size:
1144 660 305
NcoI C'CATG_G
Cuts at: 0 761 2109
Size: 761 1348
NgoMIV G'CCGG_C
Cuts at: 0 2009 2109
Size: 2009 100
NspI r_CATG'y
Cuts at: 0 15 987 1236 2109
Size: 15 972 249 873
Fragments arranged by size:
972 873 249 15
PasI CC'CwG_GG
Cuts at: 0 1159 2109
Size: 1159 950
PciI A'CATG_T
Cuts at: 0 11 983 2109
Size: 11 972 1126
Fragments arranged by size:
1126 972 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 762 2109
Size: 639 123 1347
Fragments arranged by size:
1347 639 123
PpuMI rG'GwC_Cy
Cuts at: 0 1222 2109
Size: 1222 887
PspOMI G'GGCC_C
Cuts at: 0 674 829 2109
Size: 674 155 1280
Fragments arranged by size:
1280 674 155
PstI C_TGCA'G
Cuts at: 0 688 844 2109
Size: 688 156 1265
Fragments arranged by size:
1265 688 156
PvuII CAG'CTG
Cuts at: 0 306 339 730 1033 2109
Size: 306 33 391 303 1076
Fragments arranged by size:
1076 391 306 303 33
RsrII CG'GwC_CG
Cuts at: 0 214 2109
Size: 214 1895
SacI G_AGCT'C
Cuts at: 0 89 2109
Size: 89 2020
SacII CC_GC'GG
Cuts at: 0 2059 2109
Size: 2059 50
SanDI GG'GwC_CC
Cuts at: 0 1222 2109
Size: 1222 887
ScaI AGT'ACT
Cuts at: 0 976 2109
Size: 976 1133
SfcI C'TryA_G
Cuts at: 0 684 840 2109
Size: 684 156 1269
Fragments arranged by size:
1269 684 156
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1525 2109
Size: 1525 584
SfoI GGC'GCC
Cuts at: 0 1145 1450 2109
Size: 1145 305 659
Fragments arranged by size:
1145 659 305
SmlI C'TyrA_G
Cuts at: 0 409 1621 2109
Size: 409 1212 488
Fragments arranged by size:
1212 488 409
StyI C'CwwG_G
Cuts at: 0 761 797 1697 2109
Size: 761 36 900 412
Fragments arranged by size:
900 761 412 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 2109
Size: 415 1694
TatI w'GTAC_w
Cuts at: 0 14 974 2109
Size: 14 960 1135
Fragments arranged by size:
1135 960 14
Tth111I GACn'n_nGTC
Cuts at: 0 31 402 2109
Size: 31 371 1707
Fragments arranged by size:
1707 371 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 810 2109
Size: 108 702 1299
Fragments arranged by size:
1299 702 108
XmnI GAAnn'nnTTC
Cuts at: 0 916 2109
Size: 916 1193
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PpuMI PspOMI PstI PvuII RsrII SacI
SacII SanDI ScaI SfcI SfiI SfoI SmlI StyI
TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AhdI AlfI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BclI BfrBI
BglII BmtI Bpu10I BsaBI BsiEI BsiWI BsmI BspHI
BsrDI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI
FspAI HindIII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI SalI SapI SbfI SexAI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI XbaI XhoI XmaI ZraI