Restriction map for
pFN21AE1178
(Linear) (Six-base) MAPSORT of: KIEE1178.seq Check: 2071 from: 1 to: 2058
KIEE1178.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 759 1011 1095 1500 1584 1668 1752
Size: 608 151 252 84 405 84 84 84
Cuts at: 1752 2058
Size: 306
Fragments arranged by size:
608 405 306 252 151 84 84 84
84
AleI CACnn'nnGTG
Cuts at: 0 113 1861 2058
Size: 113 1748 197
Fragments arranged by size:
1748 197 113
AlwNI CAG_nnn'CTG
Cuts at: 0 60 782 2058
Size: 60 722 1276
Fragments arranged by size:
1276 722 60
ApaI G_GGCC'C
Cuts at: 0 81 1818 2058
Size: 81 1737 240
Fragments arranged by size:
1737 240 81
ApaLI G'TGCA_C
Cuts at: 0 31 2058
Size: 31 2027
ApoI r'AATT_y
Cuts at: 0 1169 1214 1961 2045 2058
Size: 1169 45 747 84 13
Fragments arranged by size:
1169 747 84 45 13
AseI AT'TA_AT
Cuts at: 0 650 2058
Size: 650 1408
AvaI C'yCGr_G
Cuts at: 0 41 415 2058
Size: 41 374 1643
Fragments arranged by size:
1643 374 41
BanI G'GyrC_C
Cuts at: 0 225 2058
Size: 225 1833
BanII G_rGCy'C
Cuts at: 0 81 871 955 1123 1444 1780 1818 2058
Size: 81 790 84 168 321 336 38 240
Fragments arranged by size:
790 336 321 240 168 84 81 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 765 1916 2058
Size: 765 1151 142
Fragments arranged by size:
1151 765 142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 22 56 2058
Size: 22 34 2002
Fragments arranged by size:
2002 34 22
BfrBI ATG'CAT
Cuts at: 0 1149 2058
Size: 1149 909
Bme1580I G_kGCm'C
Cuts at: 0 35 81 1818 2058
Size: 35 46 1737 240
Fragments arranged by size:
1737 240 46 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 911 1079 1163 1812 2058
Size: 911 168 84 649 246
Fragments arranged by size:
911 649 246 168 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 177 2058
Size: 177 1881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 190 1328 1412 1496 1664 2058
Size: 121 69 1138 84 84 168 394
Fragments arranged by size:
1138 394 168 121 84 84 69
Bpu10I CC'TnA_GC
Cuts at: 0 152 865 2058
Size: 152 713 1193
Fragments arranged by size:
1193 713 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 939 2007 2058
Size: 939 1068 51
Fragments arranged by size:
1068 939 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 40 70 2058
Size: 40 30 1988
Fragments arranged by size:
1988 40 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 478 610 2058
Size: 61 417 132 1448
Fragments arranged by size:
1448 417 132 61
BseYI C'CCAG_C
Cuts at: 0 519 1133 2033 2058
Size: 519 614 900 25
Fragments arranged by size:
900 614 519 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1767 2058
Size: 776 991 291
Fragments arranged by size:
991 776 291
BsiHKAI G_wGCw'C
Cuts at: 0 35 871 955 1123 1612 1780 2058
Size: 35 836 84 168 489 168 278
Fragments arranged by size:
836 489 278 168 168 84 35
BsiWI C'GTAC_G
Cuts at: 0 746 2058
Size: 746 1312
BsmI GAATG_Cn'
Cuts at: 0 685 1582 1845 2058
Size: 685 897 263 213
Fragments arranged by size:
897 685 263 213
BsmBI CGTCTCn'nnnn_
Cuts at: 0 362 2058
Size: 362 1696
Bsp1286I G_dGCh'C
Cuts at: 0 35 81 871 955 1123 1444 1612 1780
Size: 35 46 790 84 168 321 168 168
Cuts at: 1780 1818 2058
Size: 38 240
Fragments arranged by size:
790 321 240 168 168 168 84 46
38 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 209 2058
Size: 209 1849
BsrDI GCAATG_nn'
Cuts at: 0 1998 2058
Size: 1998 60
BsrGI T'GTAC_A
Cuts at: 0 1336 2058
Size: 1336 722
BstYI r'GATC_y
Cuts at: 0 1271 2058
Size: 1271 787
BtgI C'CryG_G
Cuts at: 0 187 732 2058
Size: 187 545 1326
Fragments arranged by size:
1326 545 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 35 2058
Size: 35 2023
BtsI GCAGTG_nn'
Cuts at: 0 61 120 1755 2058
Size: 61 59 1635 303
Fragments arranged by size:
1635 303 61 59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1080 1115 1980 2015 2058
Size: 1080 35 865 35 43
Fragments arranged by size:
1080 865 43 35 35
DraI TTT'AAA
Cuts at: 0 834 1407 2058
Size: 834 573 651
Fragments arranged by size:
834 651 573
DraIII CAC_nnn'GTG
Cuts at: 0 845 2058
Size: 845 1213
EaeI y'GGCC_r
Cuts at: 0 69 2058
Size: 69 1989
EarI CTCTTCn'nnn_
Cuts at: 0 176 2058
Size: 176 1882
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 850 2058
Size: 850 1208
EcoICRI GAG'CTC
Cuts at: 0 869 953 1121 1778 2058
Size: 869 84 168 657 280
Fragments arranged by size:
869 657 280 168 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 190 608 759 1011 1095 1328 1412
Size: 121 69 418 151 252 84 233 84
Cuts at: 1412 1496 1500 1584 1664 1668 1752 2058
Size: 84 4 84 80 4 84 306
Fragments arranged by size:
418 306 252 233 151 121 84 84
84 84 84 80 69 4 4
EcoO109I rG'GnC_Cy
Cuts at: 0 77 78 192 233 995 1079 1652 1814
Size: 77 1 114 41 762 84 573 162
Cuts at: 1814 2058
Size: 244
Fragments arranged by size:
762 573 244 162 114 84 77 41
1
EcoRI G'AATT_C
Cuts at: 0 1214 1961 2058
Size: 1214 747 97
Fragments arranged by size:
1214 747 97
EcoRV GAT'ATC
Cuts at: 0 630 2058
Size: 630 1428
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1275 1307 1767 1799 2058
Size: 1275 32 460 32 259
Fragments arranged by size:
1275 460 259 32 32
HindIII A'AGCT_T
Cuts at: 0 938 1259 2006 2058
Size: 938 321 747 52
Fragments arranged by size:
938 747 321 52
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1731 2058
Size: 1731 327
MscI TGG'CCA
Cuts at: 0 71 2058
Size: 71 1987
MslI CAynn'nnrTG
Cuts at: 0 113 1239 1407 1861 1904 2058
Size: 113 1126 168 454 43 154
Fragments arranged by size:
1126 454 168 154 113 43
MspA1I CmG'CkG
Cuts at: 0 530 1044 1296 2058
Size: 530 514 252 762
Fragments arranged by size:
762 530 514 252
NcoI C'CATG_G
Cuts at: 0 187 732 2058
Size: 187 545 1326
Fragments arranged by size:
1326 545 187
NsiI A_TGCA'T
Cuts at: 0 1151 2058
Size: 1151 907
PasI CC'CwG_GG
Cuts at: 0 504 2058
Size: 504 1554
PpuMI rG'GwC_Cy
Cuts at: 0 192 233 2058
Size: 192 41 1825
Fragments arranged by size:
1825 192 41
PshAI GACnn'nnGTC
Cuts at: 0 322 2058
Size: 322 1736
PsiI TTA'TAA
Cuts at: 0 1322 1985 2058
Size: 1322 663 73
Fragments arranged by size:
1322 663 73
PspOMI G'GGCC_C
Cuts at: 0 77 1814 2058
Size: 77 1737 244
Fragments arranged by size:
1737 244 77
PstI C_TGCA'G
Cuts at: 0 206 2058
Size: 206 1852
PvuII CAG'CTG
Cuts at: 0 1044 1296 2058
Size: 1044 252 762
Fragments arranged by size:
1044 762 252
SacI G_AGCT'C
Cuts at: 0 871 955 1123 1780 2058
Size: 871 84 168 657 278
Fragments arranged by size:
871 657 278 168 84
SapI GCTCTTCn'nnn_
Cuts at: 0 176 2058
Size: 176 1882
SbfI CC_TGCA'GG
Cuts at: 0 206 2058
Size: 206 1852
SfcI C'TryA_G
Cuts at: 0 84 202 915 1223 2058
Size: 84 118 713 308 835
Fragments arranged by size:
835 713 308 118 84
SmaI CCC'GGG
Cuts at: 0 417 2058
Size: 417 1641
SmlI C'TyrA_G
Cuts at: 0 954 2022 2058
Size: 954 1068 36
Fragments arranged by size:
1068 954 36
StuI AGG'CCT
Cuts at: 0 277 1108 2058
Size: 277 831 950
Fragments arranged by size:
950 831 277
StyI C'CwwG_G
Cuts at: 0 187 229 491 732 2058
Size: 187 42 262 241 1326
Fragments arranged by size:
1326 262 241 187 42
SwaI ATTT'AAAT
Cuts at: 0 1407 2058
Size: 1407 651
TatI w'GTAC_w
Cuts at: 0 1336 2058
Size: 1336 722
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 40 72 2058
Size: 40 32 1986
Fragments arranged by size:
1986 40 32
XmaI C'CCGG_G
Cuts at: 0 415 2058
Size: 415 1643
XmnI GAAnn'nnTTC
Cuts at: 0 772 1024 1108 1681 2058
Size: 772 252 84 573 377
Fragments arranged by size:
772 573 377 252 84
Enzymes that do cut:
AcuI AleI AlwNI ApaI ApaLI ApoI AseI AvaI
BanI BanII BbsI BcgI BfrBI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV Hin4I HindIII
MmeI MscI MslI MspA1I NcoI NsiI PasI PpuMI
PshAI PsiI PspOMI PstI PvuII SacI SapI SbfI
SfcI SmaI SmlI StuI StyI SwaI TatI TstI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AscI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BclI BglI BglII BlpI
BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI
BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI DrdI EagI EcoNI
FalI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PspXI PsrI PvuI RsrII SacII SalI
SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI TaqII Tth111I XbaI XcmI
XhoI ZraI