Restriction map for
pFN21AE1177
(Linear) (Six-base) MAPSORT of: KIEE1177.seq Check: 8200 from: 1 to: 2052
KIEE1177.orf
With 182 enzymes: SgfI *
March 2, 2010 14:32 ..
AccI GT'mk_AC
Cuts at: 0 408 2052
Size: 408 1644
Acc65I G'GTAC_C
Cuts at: 0 720 2052
Size: 720 1332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 1319 2052
Size: 548 771 733
Fragments arranged by size:
771 733 548
AflIII A'CryG_T
Cuts at: 0 594 1480 2052
Size: 594 886 572
Fragments arranged by size:
886 594 572
AlwNI CAG_nnn'CTG
Cuts at: 0 340 1138 2052
Size: 340 798 914
Fragments arranged by size:
914 798 340
ApaI G_GGCC'C
Cuts at: 0 1747 2052
Size: 1747 305
ApoI r'AATT_y
Cuts at: 0 469 542 1049 2052
Size: 469 73 507 1003
Fragments arranged by size:
1003 507 469 73
AvaI C'yCGr_G
Cuts at: 0 129 2052
Size: 129 1923
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 712 745 2052
Size: 712 33 1307
Fragments arranged by size:
1307 712 33
BanI G'GyrC_C
Cuts at: 0 144 720 868 1377 2052
Size: 144 576 148 509 675
Fragments arranged by size:
675 576 509 148 144
BanII G_rGCy'C
Cuts at: 0 1747 2052
Size: 1747 305
BbeI G_GCGC'C
Cuts at: 0 148 2052
Size: 148 1904
BbsI GAAGACnn'nnnn_
Cuts at: 0 824 2052
Size: 824 1228
BclI T'GATC_A
Cuts at: 0 165 2052
Size: 165 1887
BfrBI ATG'CAT
Cuts at: 0 224 1123 2052
Size: 224 899 929
Fragments arranged by size:
929 899 224
BglI GCCn_nnn'nGGC
Cuts at: 0 142 251 2052
Size: 142 109 1801
Fragments arranged by size:
1801 142 109
BlpI GC'TnA_GC
Cuts at: 0 975 2052
Size: 975 1077
Bme1580I G_kGCm'C
Cuts at: 0 1747 2052
Size: 1747 305
BmgBI CAC'GTC
Cuts at: 0 1481 1875 2052
Size: 1481 394 177
Fragments arranged by size:
1481 394 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 341 2052
Size: 203 138 1711
Fragments arranged by size:
1711 203 138
Bpu10I CC'TnA_GC
Cuts at: 0 1679 2052
Size: 1679 373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1036 2052
Size: 1036 1016
BsaHI Gr'CG_yC
Cuts at: 0 145 2052
Size: 145 1907
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1222 2052
Size: 1222 830
BseYI C'CCAG_C
Cuts at: 0 449 961 2052
Size: 449 512 1091
Fragments arranged by size:
1091 512 449
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 2052
Size: 1177 875
BsiEI CG_ry'CG
Cuts at: 0 1328 1470 1774 2052
Size: 1328 142 304 278
Fragments arranged by size:
1328 304 278 142
BsmI GAATG_Cn'
Cuts at: 0 355 2052
Size: 355 1697
Bsp1286I G_dGCh'C
Cuts at: 0 1747 2052
Size: 1747 305
BspHI T'CATG_A
Cuts at: 0 1383 2052
Size: 1383 669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 600 2052
Size: 600 1452
BsrDI GCAATG_nn'
Cuts at: 0 360 2052
Size: 360 1692
BsrFI r'CCGG_y
Cuts at: 0 111 141 1220 1511 2052
Size: 111 30 1079 291 541
Fragments arranged by size:
1079 541 291 111 30
BssSI C'ACGA_G
Cuts at: 0 1286 2052
Size: 1286 766
BstAPI GCAn_nnn'nTGC
Cuts at: 0 739 1716 1939 2052
Size: 739 977 223 113
Fragments arranged by size:
977 739 223 113
BstYI r'GATC_y
Cuts at: 0 316 2052
Size: 316 1736
Bsu36I CC'TnA_GG
Cuts at: 0 62 2052
Size: 62 1990
BtgI C'CryG_G
Cuts at: 0 273 2052
Size: 273 1779
BtsI GCAGTG_nn'
Cuts at: 0 378 1165 2052
Size: 378 787 887
Fragments arranged by size:
887 787 378
ClaI AT'CG_AT
Cuts at: 0 1688 2052
Size: 1688 364
EaeI y'GGCC_r
Cuts at: 0 96 1218 1222 2052
Size: 96 1122 4 830
Fragments arranged by size:
1122 830 96 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 58 259 2052
Size: 58 201 1793
Fragments arranged by size:
1793 201 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 341 548 1319 2052
Size: 203 138 207 771 733
Fragments arranged by size:
771 733 207 203 138
EcoO109I rG'GnC_Cy
Cuts at: 0 66 1743 2052
Size: 66 1677 309
Fragments arranged by size:
1677 309 66
EcoRI G'AATT_C
Cuts at: 0 542 2052
Size: 542 1510
EcoRV GAT'ATC
Cuts at: 0 796 2052
Size: 796 1256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 872 1590 2052
Size: 872 718 462
Fragments arranged by size:
872 718 462
FseI GG_CCGG'CC
Cuts at: 0 1224 2052
Size: 1224 828
HaeII r_GCGC'y
Cuts at: 0 148 2052
Size: 148 1904
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 280 301 312 333 1244 1276 1383 1415
Size: 280 21 11 21 911 32 107 32
Cuts at: 1415 2052
Size: 637
Fragments arranged by size:
911 637 280 107 32 32 21 21
11
HincII GTy'rAC
Cuts at: 0 630 1236 1314 1734 2052
Size: 630 606 78 420 318
Fragments arranged by size:
630 606 420 318 78
KasI G'GCGC_C
Cuts at: 0 144 2052
Size: 144 1908
KpnI G_GTAC'C
Cuts at: 0 724 2052
Size: 724 1328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 587 1514 2052
Size: 156 431 927 538
Fragments arranged by size:
927 538 431 156
MslI CAynn'nnrTG
Cuts at: 0 674 1763 2052
Size: 674 1089 289
Fragments arranged by size:
1089 674 289
MspA1I CmG'CkG
Cuts at: 0 81 1722 2052
Size: 81 1641 330
Fragments arranged by size:
1641 330 81
NaeI GCC'GGC
Cuts at: 0 143 1222 2052
Size: 143 1079 830
Fragments arranged by size:
1079 830 143
NarI GG'CG_CC
Cuts at: 0 145 2052
Size: 145 1907
NcoI C'CATG_G
Cuts at: 0 273 2052
Size: 273 1779
NgoMIV G'CCGG_C
Cuts at: 0 141 1220 2052
Size: 141 1079 832
Fragments arranged by size:
1079 832 141
NsiI A_TGCA'T
Cuts at: 0 226 1125 2052
Size: 226 899 927
Fragments arranged by size:
927 899 226
NspI r_CATG'y
Cuts at: 0 598 969 1780 2052
Size: 598 371 811 272
Fragments arranged by size:
811 598 371 272
PciI A'CATG_T
Cuts at: 0 594 2052
Size: 594 1458
PfoI T'CCnGG_A
Cuts at: 0 216 2052
Size: 216 1836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1448 1480 2052
Size: 1448 32 572
Fragments arranged by size:
1448 572 32
PsiI TTA'TAA
Cuts at: 0 602 2052
Size: 602 1450
PspOMI G'GGCC_C
Cuts at: 0 1743 2052
Size: 1743 309
PstI C_TGCA'G
Cuts at: 0 385 1721 2052
Size: 385 1336 331
Fragments arranged by size:
1336 385 331
PvuI CG_AT'CG
Cuts at: 0 1470 2052
Size: 1470 582
PvuII CAG'CTG
Cuts at: 0 81 1722 2052
Size: 81 1641 330
Fragments arranged by size:
1641 330 81
SfcI C'TryA_G
Cuts at: 0 381 409 1632 1717 1985 2052
Size: 381 28 1223 85 268 67
Fragments arranged by size:
1223 381 268 85 67 28
SfoI GGC'GCC
Cuts at: 0 146 2052
Size: 146 1906
SgrAI Cr'CCGG_yG
Cuts at: 0 141 2052
Size: 141 1911
SmaI CCC'GGG
Cuts at: 0 131 2052
Size: 131 1921
SmlI C'TyrA_G
Cuts at: 0 1015 2052
Size: 1015 1037
SspI AAT'ATT
Cuts at: 0 651 2052
Size: 651 1401
StyI C'CwwG_G
Cuts at: 0 273 464 2052
Size: 273 191 1588
Fragments arranged by size:
1588 273 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1365 1595 2052
Size: 1365 230 457
Fragments arranged by size:
1365 457 230
TatI w'GTAC_w
Cuts at: 0 155 880 911 2052
Size: 155 725 31 1141
Fragments arranged by size:
1141 725 155 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 76 108 2052
Size: 76 32 1944
Fragments arranged by size:
1944 76 32
XmaI C'CCGG_G
Cuts at: 0 129 2052
Size: 129 1923
XmnI GAAnn'nnTTC
Cuts at: 0 1460 2052
Size: 1460 592
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlwNI ApaI ApoI AvaI
BaeI BanI BanII BbeI BbsI BclI BfrBI BglI
BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BseRI
BseYI BsgI BsiEI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtsI ClaI
EaeI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FseI
HaeII Hin4I HincII KasI KpnI MmeI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI
PpiI PsiI PspOMI PstI PvuI PvuII SfcI SfoI
SgrAI SmaI SmlI SspI StyI TaqII TatI TstI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BamHI
BbvCI BcgI BciVI BglII BmrI BmtI BplI BsaI
BsaAI BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmBI BspEI
BsrBI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgZI
CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI
FspI FspAI HindIII HpaI MfeI MluI MscI NdeI
NheI NotI NruI PacI PasI PflMI PmeI PmlI
PpuMI PshAI PspXI PsrI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XcmI
XhoI ZraI