Restriction map for
pFN21AE1172
(Linear) (Six-base) MAPSORT of: KIEE1172.seq Check: 4214 from: 1 to: 1884
KIEE1172.orf
With 182 enzymes: SgfI *
March 22, 2012 15:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 1404 1488 1824 1884
Size: 732 672 84 336 60
Fragments arranged by size:
732 672 336 84 60
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 118 150 1884
Size: 118 32 1734
Fragments arranged by size:
1734 118 32
ApoI r'AATT_y
Cuts at: 0 25 383 449 782 866 950 1202 1454
Size: 25 358 66 333 84 84 252 252
Cuts at: 1454 1538 1790 1884
Size: 84 252 94
Fragments arranged by size:
358 333 252 252 252 94 84 84
84 66 25
BanII G_rGCy'C
Cuts at: 0 280 1884
Size: 280 1604
BbsI GAAGACnn'nnnn_
Cuts at: 0 142 906 1410 1424 1494 1760 1830 1884
Size: 142 764 504 14 70 266 70 54
Fragments arranged by size:
764 504 266 142 70 70 54 14
BclI T'GATC_A
Cuts at: 0 501 1884
Size: 501 1383
BfrBI ATG'CAT
Cuts at: 0 702 1884
Size: 702 1182
BglII A'GATC_T
Cuts at: 0 1247 1751 1884
Size: 1247 504 133
Fragments arranged by size:
1247 504 133
BmgBI CAC'GTC
Cuts at: 0 105 1884
Size: 105 1779
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 294 1884
Size: 294 1590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 812 896 980 1484 1652 1884
Size: 728 84 84 84 504 168 232
Fragments arranged by size:
728 504 232 168 84 84 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 138 1212 1884
Size: 138 1074 672
Fragments arranged by size:
1074 672 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 118 148 1884
Size: 118 30 1736
Fragments arranged by size:
1736 118 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 288 1884
Size: 288 1596
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1848 1884
Size: 1848 36
BsiHKAI G_wGCw'C
Cuts at: 0 280 572 1884
Size: 280 292 1312
Fragments arranged by size:
1312 292 280
BsmI GAATG_Cn'
Cuts at: 0 704 1884
Size: 704 1180
Bsp1286I G_dGCh'C
Cuts at: 0 280 572 1884
Size: 280 292 1312
Fragments arranged by size:
1312 292 280
BspHI T'CATG_A
Cuts at: 0 729 813 897 1728 1884
Size: 729 84 84 831 156
Fragments arranged by size:
831 729 156 84 84
BstAPI GCAn_nnn'nTGC
Cuts at: 0 248 1826 1884
Size: 248 1578 58
Fragments arranged by size:
1578 248 58
BstBI TT'CG_AA
Cuts at: 0 834 1884
Size: 834 1050
BstYI r'GATC_y
Cuts at: 0 192 1247 1751 1884
Size: 192 1055 504 133
Fragments arranged by size:
1055 504 192 133
BtsI GCAGTG_nn'
Cuts at: 0 651 1884
Size: 651 1233
DraI TTT'AAA
Cuts at: 0 479 1884
Size: 479 1405
DraIII CAC_nnn'GTG
Cuts at: 0 1826 1884
Size: 1826 58
EaeI y'GGCC_r
Cuts at: 0 38 573 1884
Size: 38 535 1311
Fragments arranged by size:
1311 535 38
EarI CTCTTCn'nnn_
Cuts at: 0 53 209 751 1884
Size: 53 156 542 1133
Fragments arranged by size:
1133 542 156 53
EcoICRI GAG'CTC
Cuts at: 0 278 1884
Size: 278 1606
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 732 812 896 980 1404 1484 1488
Size: 728 4 80 84 84 424 80 4
Cuts at: 1488 1652 1824 1884
Size: 164 172 60
Fragments arranged by size:
728 424 172 164 84 84 80 80
60 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 1884
Size: 10 1874
EcoO109I rG'GnC_Cy
Cuts at: 0 16 1884
Size: 16 1868
EcoRI G'AATT_C
Cuts at: 0 782 866 1202 1454 1790 1884
Size: 782 84 336 252 336 94
Fragments arranged by size:
782 336 336 252 94 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1154 1884
Size: 1154 730
FspI TGC'GCA
Cuts at: 0 83 1884
Size: 83 1801
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 1179 1211 1239 1271 1743 1775
Size: 412 32 735 32 28 32 472 32
Cuts at: 1775 1884
Size: 109
Fragments arranged by size:
735 472 412 109 32 32 32 32
28
HindIII A'AGCT_T
Cuts at: 0 551 679 847 1884
Size: 551 128 168 1037
Fragments arranged by size:
1037 551 168 128
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1884
Size: 92 1792
MscI TGG'CCA
Cuts at: 0 40 1884
Size: 40 1844
MslI CAynn'nnrTG
Cuts at: 0 34 350 734 818 902 1043 1295 1884
Size: 34 316 384 84 84 141 252 589
Fragments arranged by size:
589 384 316 252 141 84 84 34
NsiI A_TGCA'T
Cuts at: 0 704 1884
Size: 704 1180
PasI CC'CwG_GG
Cuts at: 0 138 1884
Size: 138 1746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 1884
Size: 118 32 1734
Fragments arranged by size:
1734 118 32
PpuMI rG'GwC_Cy
Cuts at: 0 16 1884
Size: 16 1868
PsiI TTA'TAA
Cuts at: 0 557 1884
Size: 557 1327
PstI C_TGCA'G
Cuts at: 0 209 1884
Size: 209 1675
SacI G_AGCT'C
Cuts at: 0 280 1884
Size: 280 1604
SanDI GG'GwC_CC
Cuts at: 0 16 1884
Size: 16 1868
SapI GCTCTTCn'nnn_
Cuts at: 0 751 1884
Size: 751 1133
SexAI A'CCwGG_T
Cuts at: 0 128 1520 1884
Size: 128 1392 364
Fragments arranged by size:
1392 364 128
SfcI C'TryA_G
Cuts at: 0 205 358 1884
Size: 205 153 1526
Fragments arranged by size:
1526 205 153
StuI AGG'CCT
Cuts at: 0 1641 1688 1884
Size: 1641 47 196
Fragments arranged by size:
1641 196 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 380 1884
Size: 380 1504
Tth111I GACn'n_nGTC
Cuts at: 0 826 910 1162 1884
Size: 826 84 252 722
Fragments arranged by size:
826 722 252 84
XmnI GAAnn'nnTTC
Cuts at: 0 840 1884
Size: 840 1044
Enzymes that do cut:
AcuI AloI ApoI BanII BbsI BclI BfrBI BglII
BmgBI BplI BpmI BsaI BsaXI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspHI BstAPI BstBI BstYI BtsI DraI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI Hin4I HindIII MmeI MscI MslI NsiI
PasI PpiI PpuMI PsiI PstI SacI SanDI SapI
SexAI SfcI StuI TaqII Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BglI BlpI Bme1580I BmrI BmtI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DrdI EagI EciI EcoRV FseI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PacI PciI PflMI PfoI PmeI PmlI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TatI
TstI XbaI XcmI XhoI XmaI ZraI