Restriction map for
pFN21AE1156
(Linear) (Six-base) MAPSORT of: KIEE1156.seq Check: 7922 from: 1 to: 1392
KIEE1156.orf
With 182 enzymes: SgfI *
July 3, 2012 16:47 ..
AccI GT'mk_AC
Cuts at: 0 62 1392
Size: 62 1330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 142 1392
Size: 142 1250
AlwNI CAG_nnn'CTG
Cuts at: 0 986 1392
Size: 986 406
ApoI r'AATT_y
Cuts at: 0 910 994 1092 1162 1260 1392
Size: 910 84 98 70 98 132
Fragments arranged by size:
910 132 98 98 84 70
BanII G_rGCy'C
Cuts at: 0 430 1392
Size: 430 962
BbvCI CC'TCA_GC
Cuts at: 0 182 1392
Size: 182 1210
BclI T'GATC_A
Cuts at: 0 368 1392
Size: 368 1024
BmrI ACTGGGnnnn_n'
Cuts at: 0 289 1392
Size: 289 1103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 644 728 812 828 896 980 1064
Size: 151 493 84 84 16 68 84 84
Cuts at: 1064 1148 1316 1332 1392
Size: 84 168 16 60
Fragments arranged by size:
493 168 151 84 84 84 84 84
68 60 16 16
Bpu10I CC'TnA_GC
Cuts at: 0 29 182 194 375 1392
Size: 29 153 12 181 1017
Fragments arranged by size:
1017 181 153 29 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 996 1392
Size: 370 626 396
Fragments arranged by size:
626 396 370
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 199 318 1392
Size: 100 99 119 1074
Fragments arranged by size:
1074 119 100 99
BseYI C'CCAG_C
Cuts at: 0 538 1392
Size: 538 854
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 831 915 999 1167 1335 1392
Size: 831 84 84 168 168 57
Fragments arranged by size:
831 168 168 84 84 57
BsiEI CG_ry'CG
Cuts at: 0 11 1392
Size: 11 1381
BsiHKAI G_wGCw'C
Cuts at: 0 430 1392
Size: 430 962
BsmI GAATG_Cn'
Cuts at: 0 562 1392
Size: 562 830
Bsp1286I G_dGCh'C
Cuts at: 0 430 1392
Size: 430 962
BspHI T'CATG_A
Cuts at: 0 851 1392
Size: 851 541
BsrBI CCG'CTC
Cuts at: 0 28 1392
Size: 28 1364
BsrDI GCAATG_nn'
Cuts at: 0 126 1071 1239 1392
Size: 126 945 168 153
Fragments arranged by size:
945 168 153 126
Bsu36I CC'TnA_GG
Cuts at: 0 16 1392
Size: 16 1376
BtsI GCAGTG_nn'
Cuts at: 0 483 567 735 819 903 1392
Size: 483 84 168 84 84 489
Fragments arranged by size:
489 483 168 84 84 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1053 1088 1221 1256 1392
Size: 1053 35 133 35 136
Fragments arranged by size:
1053 136 133 35 35
DraI TTT'AAA
Cuts at: 0 1359 1392
Size: 1359 33
DraIII CAC_nnn'GTG
Cuts at: 0 396 1392
Size: 396 996
EaeI y'GGCC_r
Cuts at: 0 8 1392
Size: 8 1384
EagI C'GGCC_G
Cuts at: 0 8 1392
Size: 8 1384
EcoICRI GAG'CTC
Cuts at: 0 428 1392
Size: 428 964
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 644 728 812 828 896 980 1064
Size: 151 493 84 84 16 68 84 84
Cuts at: 1064 1148 1316 1332 1392
Size: 84 168 16 60
Fragments arranged by size:
493 168 151 84 84 84 84 84
68 60 16 16
EcoO109I rG'GnC_Cy
Cuts at: 0 21 291 360 1392
Size: 21 270 69 1032
Fragments arranged by size:
1032 270 69 21
EcoRI G'AATT_C
Cuts at: 0 1092 1260 1392
Size: 1092 168 132
Fragments arranged by size:
1092 168 132
HindIII A'AGCT_T
Cuts at: 0 743 1392
Size: 743 649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 953 1392
Size: 617 336 439
Fragments arranged by size:
617 439 336
MslI CAynn'nnrTG
Cuts at: 0 947 1392
Size: 947 445
NotI GC'GGCC_GC
Cuts at: 0 8 1392
Size: 8 1384
NspI r_CATG'y
Cuts at: 0 538 728 1392
Size: 538 190 664
Fragments arranged by size:
664 538 190
PpuMI rG'GwC_Cy
Cuts at: 0 21 291 1392
Size: 21 270 1101
Fragments arranged by size:
1101 270 21
PsiI TTA'TAA
Cuts at: 0 890 1392
Size: 890 502
SacI G_AGCT'C
Cuts at: 0 430 1392
Size: 430 962
SanDI GG'GwC_CC
Cuts at: 0 21 291 1392
Size: 21 270 1101
Fragments arranged by size:
1101 270 21
SexAI A'CCwGG_T
Cuts at: 0 392 1392
Size: 392 1000
SfcI C'TryA_G
Cuts at: 0 209 915 1392
Size: 209 706 477
Fragments arranged by size:
706 477 209
SmlI C'TyrA_G
Cuts at: 0 385 1011 1392
Size: 385 626 381
Fragments arranged by size:
626 385 381
SspI AAT'ATT
Cuts at: 0 508 1392
Size: 508 884
StuI AGG'CCT
Cuts at: 0 445 633 801 969 1392
Size: 445 188 168 168 423
Fragments arranged by size:
445 423 188 168 168
Enzymes that do cut:
AccI AlfI AlwNI ApoI BanII BbvCI BclI BmrI
BpmI Bpu10I BpuEI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BsrBI BsrDI Bsu36I BtsI CspCI
DraI DraIII EaeI EagI EcoICRI Eco57MI EcoO109I EcoRI
HindIII MmeI MslI NotI NspI PpuMI PsiI SacI
SanDI SexAI SfcI SmlI SspI StuI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiWI BsmBI BspEI BspMI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
ClaI DrdI EarI EciI EcoNI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI