Restriction map for
pFN21AE1153
(Linear) (Six-base) MAPSORT of: KIEE1153.seq Check: 8696 from: 1 to: 1500
KIEE1153.orf
With 182 enzymes: SgfI *
October 29, 2012 16:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 1136 1500
Size: 210 926 364
Fragments arranged by size:
926 364 210
AlwNI CAG_nnn'CTG
Cuts at: 0 318 570 790 1500
Size: 318 252 220 710
Fragments arranged by size:
710 318 252 220
ApaI G_GGCC'C
Cuts at: 0 78 112 1500
Size: 78 34 1388
Fragments arranged by size:
1388 78 34
ApaLI G'TGCA_C
Cuts at: 0 890 1500
Size: 890 610
ApoI r'AATT_y
Cuts at: 0 342 424 508 527 1500
Size: 342 82 84 19 973
Fragments arranged by size:
973 342 84 82 19
BanI G'GyrC_C
Cuts at: 0 86 1500
Size: 86 1414
BanII G_rGCy'C
Cuts at: 0 78 112 377 701 1118 1500
Size: 78 34 265 324 417 382
Fragments arranged by size:
417 382 324 265 78 34
BbeI G_GCGC'C
Cuts at: 0 90 1500
Size: 90 1410
BbsI GAAGACnn'nnnn_
Cuts at: 0 1319 1500
Size: 1319 181
BglI GCCn_nnn'nGGC
Cuts at: 0 724 1500
Size: 724 776
BlpI GC'TnA_GC
Cuts at: 0 371 1500
Size: 371 1129
Bme1580I G_kGCm'C
Cuts at: 0 78 112 894 1500
Size: 78 34 782 606
Fragments arranged by size:
782 606 78 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 1416 1500
Size: 1416 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1056 1500
Size: 1056 444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 694 1217 1400 1500
Size: 427 267 523 183 100
Fragments arranged by size:
523 427 267 183 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 735 935 1500
Size: 154 581 200 565
Fragments arranged by size:
581 565 200 154
BsaI GGTCTCn'nnnn_
Cuts at: 0 637 981 1500
Size: 637 344 519
Fragments arranged by size:
637 519 344
BsaHI Gr'CG_yC
Cuts at: 0 55 87 692 1500
Size: 55 32 605 808
Fragments arranged by size:
808 605 55 32
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 23 697 1108 1125 1500
Size: 23 674 411 17 375
Fragments arranged by size:
674 411 375 23 17
BseYI C'CCAG_C
Cuts at: 0 102 161 960 1117 1500
Size: 102 59 799 157 383
Fragments arranged by size:
799 383 157 102 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1405 1500
Size: 1405 95
BsiHKAI G_wGCw'C
Cuts at: 0 170 284 377 894 1500
Size: 170 114 93 517 606
Fragments arranged by size:
606 517 170 114 93
Bsp1286I G_dGCh'C
Cuts at: 0 78 112 170 284 377 701 894 1118
Size: 78 34 58 114 93 324 193 224
Cuts at: 1118 1500
Size: 382
Fragments arranged by size:
382 324 224 193 114 93 78 58
34
BsrFI r'CCGG_y
Cuts at: 0 212 1500
Size: 212 1288
BssSI C'ACGA_G
Cuts at: 0 91 184 1500
Size: 91 93 1316
Fragments arranged by size:
1316 93 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 255 660 913 1500
Size: 255 405 253 587
Fragments arranged by size:
587 405 255 253
BstYI r'GATC_y
Cuts at: 0 233 933 1500
Size: 233 700 567
Fragments arranged by size:
700 567 233
BtsI GCAGTG_nn'
Cuts at: 0 319 449 1500
Size: 319 130 1051
Fragments arranged by size:
1051 319 130
DraIII CAC_nnn'GTG
Cuts at: 0 288 1500
Size: 288 1212
EaeI y'GGCC_r
Cuts at: 0 716 1500
Size: 716 784
EarI CTCTTCn'nnn_
Cuts at: 0 140 1268 1500
Size: 140 1128 232
Fragments arranged by size:
1128 232 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 130 1488 1500
Size: 130 1358 12
Fragments arranged by size:
1358 130 12
EcoICRI GAG'CTC
Cuts at: 0 375 1500
Size: 375 1125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 427 694 1136 1217 1400 1500
Size: 210 217 267 442 81 183 100
Fragments arranged by size:
442 267 217 210 183 100 81
EcoO109I rG'GnC_Cy
Cuts at: 0 108 266 552 787 1500
Size: 108 158 286 235 713
Fragments arranged by size:
713 286 235 158 108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 195 1500
Size: 195 1305
HaeII r_GCGC'y
Cuts at: 0 90 193 1159 1500
Size: 90 103 966 341
Fragments arranged by size:
966 341 103 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 564 596 1449 1481 1500
Size: 564 32 853 32 19
Fragments arranged by size:
853 564 32 32 19
HincII GTy'rAC
Cuts at: 0 1440 1500
Size: 1440 60
HpaI GTT'AAC
Cuts at: 0 1440 1500
Size: 1440 60
KasI G'GCGC_C
Cuts at: 0 86 1500
Size: 86 1414
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1500
Size: 862 638
MscI TGG'CCA
Cuts at: 0 718 1500
Size: 718 782
MslI CAynn'nnrTG
Cuts at: 0 686 850 1500
Size: 686 164 650
Fragments arranged by size:
686 650 164
MspA1I CmG'CkG
Cuts at: 0 32 43 745 1450 1500
Size: 32 11 702 705 50
Fragments arranged by size:
705 702 50 32 11
NarI GG'CG_CC
Cuts at: 0 87 1500
Size: 87 1413
NdeI CA'TA_TG
Cuts at: 0 542 639 1500
Size: 542 97 861
Fragments arranged by size:
861 542 97
PfoI T'CCnGG_A
Cuts at: 0 236 671 1500
Size: 236 435 829
Fragments arranged by size:
829 435 236
PpuMI rG'GwC_Cy
Cuts at: 0 552 1500
Size: 552 948
PspOMI G'GGCC_C
Cuts at: 0 74 108 1500
Size: 74 34 1392
Fragments arranged by size:
1392 74 34
PstI C_TGCA'G
Cuts at: 0 1052 1500
Size: 1052 448
PvuII CAG'CTG
Cuts at: 0 43 1450 1500
Size: 43 1407 50
Fragments arranged by size:
1407 50 43
SacI G_AGCT'C
Cuts at: 0 377 1500
Size: 377 1123
ScaI AGT'ACT
Cuts at: 0 1110 1500
Size: 1110 390
SfcI C'TryA_G
Cuts at: 0 1048 1352 1500
Size: 1048 304 148
Fragments arranged by size:
1048 304 148
SfoI GGC'GCC
Cuts at: 0 88 1500
Size: 88 1412
SmlI C'TyrA_G
Cuts at: 0 169 750 950 1500
Size: 169 581 200 550
Fragments arranged by size:
581 550 200 169
SspI AAT'ATT
Cuts at: 0 591 1500
Size: 591 909
StuI AGG'CCT
Cuts at: 0 1127 1500
Size: 1127 373
StyI C'CwwG_G
Cuts at: 0 269 548 1500
Size: 269 279 952
Fragments arranged by size:
952 279 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 153 819 1257 1500
Size: 153 666 438 243
Fragments arranged by size:
666 438 243 153
TatI w'GTAC_w
Cuts at: 0 754 1108 1318 1500
Size: 754 354 210 182
Fragments arranged by size:
754 354 210 182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 274 306 1500
Size: 274 32 1194
Fragments arranged by size:
1194 274 32
Tth111I GACn'n_nGTC
Cuts at: 0 882 1500
Size: 882 618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 462 1192 1500
Size: 462 730 308
Fragments arranged by size:
730 462 308
XmnI GAAnn'nnTTC
Cuts at: 0 1286 1500
Size: 1286 214
Enzymes that do cut:
AcuI AlwNI ApaI ApaLI ApoI BanI BanII BbeI
BbsI BglI BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrFI
BssSI BstAPI BstYI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII HpaI
KasI MmeI MscI MslI MspA1I NarI NdeI PfoI
PpuMI PspOMI PstI PvuII SacI ScaI SfcI SfoI
SmlI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmtI Bpu10I BsaAI BsaBI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI
EcoNI EcoRI EcoRV FseI FspI FspAI HindIII KpnI
MfeI MluI NaeI NcoI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI XbaI XhoI
XmaI ZraI