Restriction map for
pFN21AE1152
(Linear) (Six-base) MAPSORT of: KIEE1152.seq Check: 3775 from: 1 to: 1464
KIEE1152.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 1193 1464
Size: 614 579 271
Fragments arranged by size:
614 579 271
AflIII A'CryG_T
Cuts at: 0 69 268 1464
Size: 69 199 1196
Fragments arranged by size:
1196 199 69
AleI CACnn'nnGTG
Cuts at: 0 28 1464
Size: 28 1436
AlwNI CAG_nnn'CTG
Cuts at: 0 761 917 1464
Size: 761 156 547
Fragments arranged by size:
761 547 156
AvaI C'yCGr_G
Cuts at: 0 842 1464
Size: 842 622
BanII G_rGCy'C
Cuts at: 0 510 733 1464
Size: 510 223 731
Fragments arranged by size:
731 510 223
BbsI GAAGACnn'nnnn_
Cuts at: 0 416 1464
Size: 416 1048
BglII A'GATC_T
Cuts at: 0 420 1464
Size: 420 1044
Bme1580I G_kGCm'C
Cuts at: 0 882 1464
Size: 882 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1464
Size: 71 1393
BsaAI yAC'GTr
Cuts at: 0 279 774 1464
Size: 279 495 690
Fragments arranged by size:
690 495 279
BsaWI w'CCGG_w
Cuts at: 0 698 1143 1464
Size: 698 445 321
Fragments arranged by size:
698 445 321
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1347 1377 1464
Size: 1347 30 87
Fragments arranged by size:
1347 87 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1232 1355 1464
Size: 1232 123 109
Fragments arranged by size:
1232 123 109
BseYI C'CCAG_C
Cuts at: 0 43 1464
Size: 43 1421
BsiHKAI G_wGCw'C
Cuts at: 0 247 1058 1464
Size: 247 811 406
Fragments arranged by size:
811 406 247
BsmI GAATG_Cn'
Cuts at: 0 180 1464
Size: 180 1284
Bsp1286I G_dGCh'C
Cuts at: 0 247 510 733 882 1058 1464
Size: 247 263 223 149 176 406
Fragments arranged by size:
406 263 247 223 176 149
BspEI T'CCGG_A
Cuts at: 0 698 1464
Size: 698 766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 135 183 1464
Size: 135 48 1281
Fragments arranged by size:
1281 135 48
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1431 1464
Size: 1431 33
BstYI r'GATC_y
Cuts at: 0 420 1464
Size: 420 1044
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 918 953 1464
Size: 918 35 511
Fragments arranged by size:
918 511 35
DraI TTT'AAA
Cuts at: 0 680 1464
Size: 680 784
EaeI y'GGCC_r
Cuts at: 0 157 1464
Size: 157 1307
EarI CTCTTCn'nnn_
Cuts at: 0 290 821 845 1464
Size: 290 531 24 619
Fragments arranged by size:
619 531 290 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 614 1193 1464
Size: 71 543 579 271
Fragments arranged by size:
579 543 271 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 190 1464
Size: 190 1274
EcoO109I rG'GnC_Cy
Cuts at: 0 195 1464
Size: 195 1269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 566 598 728 760 931 963 1464
Size: 566 32 130 32 171 32 501
Fragments arranged by size:
566 501 171 130 32 32 32
HincII GTy'rAC
Cuts at: 0 960 1464
Size: 960 504
MfeI C'AATT_G
Cuts at: 0 1328 1464
Size: 1328 136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1464
Size: 640 824
MscI TGG'CCA
Cuts at: 0 159 1464
Size: 159 1305
MslI CAynn'nnrTG
Cuts at: 0 28 173 865 1096 1464
Size: 28 145 692 231 368
Fragments arranged by size:
692 368 231 145 28
MspA1I CmG'CkG
Cuts at: 0 982 1464
Size: 982 482
NspI r_CATG'y
Cuts at: 0 73 272 718 1464
Size: 73 199 446 746
Fragments arranged by size:
746 446 199 73
PciI A'CATG_T
Cuts at: 0 69 268 1464
Size: 69 199 1196
Fragments arranged by size:
1196 199 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 368 761 917 1464
Size: 368 393 156 547
Fragments arranged by size:
547 393 368 156
PfoI T'CCnGG_A
Cuts at: 0 428 459 1464
Size: 428 31 1005
Fragments arranged by size:
1005 428 31
PpuMI rG'GwC_Cy
Cuts at: 0 195 1464
Size: 195 1269
PstI C_TGCA'G
Cuts at: 0 894 1009 1464
Size: 894 115 455
Fragments arranged by size:
894 455 115
PvuII CAG'CTG
Cuts at: 0 982 1464
Size: 982 482
SfcI C'TryA_G
Cuts at: 0 890 1005 1181 1464
Size: 890 115 176 283
Fragments arranged by size:
890 283 176 115
SmaI CCC'GGG
Cuts at: 0 844 1464
Size: 844 620
SnaBI TAC'GTA
Cuts at: 0 279 774 1464
Size: 279 495 690
Fragments arranged by size:
690 495 279
SspI AAT'ATT
Cuts at: 0 391 1464
Size: 391 1073
StyI C'CwwG_G
Cuts at: 0 500 1464
Size: 500 964
TatI w'GTAC_w
Cuts at: 0 481 1464
Size: 481 983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 167 1464
Size: 167 1297
XmaI C'CCGG_G
Cuts at: 0 842 1464
Size: 842 622
XmnI GAAnn'nnTTC
Cuts at: 0 299 1464
Size: 299 1165
Enzymes that do cut:
AcuI AflIII AleI AlwNI AvaI BanII BbsI BglII
Bme1580I BpmI BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspEI BspMI BstXI BstYI CspCI DraI
EaeI EarI Eco57MI EcoNI EcoO109I Hin4I HincII MfeI
MmeI MscI MslI MspA1I NspI PciI PflMI PfoI
PpuMI PstI PvuII SfcI SmaI SnaBI SspI StyI
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsgI BsiEI
BsiWI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SpeI SphI SrfI
StuI SwaI TaqII TstI Tth111I XbaI XhoI ZraI