Restriction map for
pFN21AE1151
(Linear) (Six-base) MAPSORT of: KIEE1151.seq Check: 1095 from: 1 to: 1458
KIEE1151.orf
With 182 enzymes: SgfI *
July 3, 2012 16:47 ..
AccI GT'mk_AC
Cuts at: 0 161 1458
Size: 161 1297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 706 838 1458
Size: 528 178 132 620
Fragments arranged by size:
620 528 178 132
AfeI AGC'GCT
Cuts at: 0 541 1458
Size: 541 917
AflII C'TTAA_G
Cuts at: 0 855 1458
Size: 855 603
AlwNI CAG_nnn'CTG
Cuts at: 0 104 642 1458
Size: 104 538 816
Fragments arranged by size:
816 538 104
ApaI G_GGCC'C
Cuts at: 0 187 272 1149 1458
Size: 187 85 877 309
Fragments arranged by size:
877 309 187 85
ApoI r'AATT_y
Cuts at: 0 1262 1458
Size: 1262 196
AvaI C'yCGr_G
Cuts at: 0 16 198 271 364 903 1458
Size: 16 182 73 93 539 555
Fragments arranged by size:
555 539 182 93 73 16
BamHI G'GATC_C
Cuts at: 0 885 1458
Size: 885 573
BanI G'GyrC_C
Cuts at: 0 9 44 387 716 1458
Size: 9 35 343 329 742
Fragments arranged by size:
742 343 329 35 9
BanII G_rGCy'C
Cuts at: 0 187 272 854 1149 1458
Size: 187 85 582 295 309
Fragments arranged by size:
582 309 295 187 85
BbeI G_GCGC'C
Cuts at: 0 13 391 1458
Size: 13 378 1067
Fragments arranged by size:
1067 378 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 826 1318 1458
Size: 826 492 140
Fragments arranged by size:
826 492 140
BbvCI CC'TCA_GC
Cuts at: 0 601 1458
Size: 601 857
BclI T'GATC_A
Cuts at: 0 1449 1458
Size: 1449 9
Bme1580I G_kGCm'C
Cuts at: 0 49 187 272 1149 1458
Size: 49 138 85 877 309
Fragments arranged by size:
877 309 138 85 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 1050 1458
Size: 1050 408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 631 763 1458
Size: 412 219 132 695
Fragments arranged by size:
695 412 219 132
Bpu10I CC'TnA_GC
Cuts at: 0 307 601 763 977 1458
Size: 307 294 162 214 481
Fragments arranged by size:
481 307 294 214 162
BsaBI GATnn'nnATC
Cuts at: 0 1451 1458
Size: 1451 7
BsaHI Gr'CG_yC
Cuts at: 0 10 158 356 388 1458
Size: 10 148 198 32 1070
Fragments arranged by size:
1070 198 148 32 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 601 634 770 858 1352 1458
Size: 337 264 33 136 88 494 106
Fragments arranged by size:
494 337 264 136 106 88 33
BseYI C'CCAG_C
Cuts at: 0 252 344 1458
Size: 252 92 1114
Fragments arranged by size:
1114 252 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 958 1458
Size: 958 500
BsiHKAI G_wGCw'C
Cuts at: 0 371 854 1458
Size: 371 483 604
Fragments arranged by size:
604 483 371
Bsp1286I G_dGCh'C
Cuts at: 0 49 187 272 371 854 1149 1458
Size: 49 138 85 99 483 295 309
Fragments arranged by size:
483 309 295 138 99 85 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 935 1458
Size: 935 523
BsrBI CCG'CTC
Cuts at: 0 24 1458
Size: 24 1434
BsrFI r'CCGG_y
Cuts at: 0 75 215 358 406 659 1255 1458
Size: 75 140 143 48 253 596 203
Fragments arranged by size:
596 253 203 143 140 75 48
BssHII G'CGCG_C
Cuts at: 0 146 287 1458
Size: 146 141 1171
Fragments arranged by size:
1171 146 141
BstAPI GCAn_nnn'nTGC
Cuts at: 0 642 1458
Size: 642 816
BstYI r'GATC_y
Cuts at: 0 885 1288 1425 1458
Size: 885 403 137 33
Fragments arranged by size:
885 403 137 33
Bsu36I CC'TnA_GG
Cuts at: 0 109 1458
Size: 109 1349
BtgI C'CryG_G
Cuts at: 0 809 893 952 1458
Size: 809 84 59 506
Fragments arranged by size:
809 506 84 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 297 1458
Size: 297 1161
BtsI GCAGTG_nn'
Cuts at: 0 181 939 1096 1458
Size: 181 758 157 362
Fragments arranged by size:
758 362 181 157
EaeI y'GGCC_r
Cuts at: 0 93 1458
Size: 93 1365
EarI CTCTTCn'nnn_
Cuts at: 0 1208 1321 1458
Size: 1208 113 137
Fragments arranged by size:
1208 137 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 631 712 1458
Size: 631 81 746
Fragments arranged by size:
746 631 81
EcoICRI GAG'CTC
Cuts at: 0 852 1458
Size: 852 606
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 528 631 706 763 838 1458
Size: 412 116 103 75 57 75 620
Fragments arranged by size:
620 412 116 103 75 75 57
EcoO109I rG'GnC_Cy
Cuts at: 0 183 184 268 303 681 1146 1458
Size: 183 1 84 35 378 465 312
Fragments arranged by size:
465 378 312 183 84 35 1
FspI TGC'GCA
Cuts at: 0 931 1458
Size: 931 527
HaeII r_GCGC'y
Cuts at: 0 13 340 391 543 726 1458
Size: 13 327 51 152 183 732
Fragments arranged by size:
732 327 183 152 51 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1280 1312 1458
Size: 1280 32 146
Fragments arranged by size:
1280 146 32
KasI G'GCGC_C
Cuts at: 0 9 387 1458
Size: 9 378 1071
Fragments arranged by size:
1071 378 9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 440 1233 1458
Size: 119 321 793 225
Fragments arranged by size:
793 321 225 119
MslI CAynn'nnrTG
Cuts at: 0 808 1458
Size: 808 650
MspA1I CmG'CkG
Cuts at: 0 222 393 600 1458
Size: 222 171 207 858
Fragments arranged by size:
858 222 207 171
NaeI GCC'GGC
Cuts at: 0 360 408 661 1458
Size: 360 48 253 797
Fragments arranged by size:
797 360 253 48
NarI GG'CG_CC
Cuts at: 0 10 388 1458
Size: 10 378 1070
Fragments arranged by size:
1070 378 10
NcoI C'CATG_G
Cuts at: 0 809 952 1458
Size: 809 143 506
Fragments arranged by size:
809 506 143
NgoMIV G'CCGG_C
Cuts at: 0 358 406 659 1458
Size: 358 48 253 799
Fragments arranged by size:
799 358 253 48
NspI r_CATG'y
Cuts at: 0 830 1458
Size: 830 628
PasI CC'CwG_GG
Cuts at: 0 732 838 1458
Size: 732 106 620
Fragments arranged by size:
732 620 106
PpuMI rG'GwC_Cy
Cuts at: 0 303 1458
Size: 303 1155
PspOMI G'GGCC_C
Cuts at: 0 183 268 1145 1458
Size: 183 85 877 313
Fragments arranged by size:
877 313 183 85
PstI C_TGCA'G
Cuts at: 0 71 398 638 710 1458
Size: 71 327 240 72 748
Fragments arranged by size:
748 327 240 72 71
PvuII CAG'CTG
Cuts at: 0 222 600 1458
Size: 222 378 858
Fragments arranged by size:
858 378 222
SacI G_AGCT'C
Cuts at: 0 854 1458
Size: 854 604
SbfI CC_TGCA'GG
Cuts at: 0 71 1458
Size: 71 1387
SexAI A'CCwGG_T
Cuts at: 0 416 1458
Size: 416 1042
SfcI C'TryA_G
Cuts at: 0 67 394 634 706 1458
Size: 67 327 240 72 752
Fragments arranged by size:
752 327 240 72 67
SfoI GGC'GCC
Cuts at: 0 11 389 1458
Size: 11 378 1069
Fragments arranged by size:
1069 378 11
SmaI CCC'GGG
Cuts at: 0 18 273 1458
Size: 18 255 1185
Fragments arranged by size:
1185 255 18
SmlI C'TyrA_G
Cuts at: 0 855 1458
Size: 855 603
SphI G_CATG'C
Cuts at: 0 830 1458
Size: 830 628
StyI C'CwwG_G
Cuts at: 0 809 952 1458
Size: 809 143 506
Fragments arranged by size:
809 506 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 336 1392 1458
Size: 336 1056 66
Fragments arranged by size:
1056 336 66
TatI w'GTAC_w
Cuts at: 0 1172 1458
Size: 1172 286
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 60 1458
Size: 28 32 1398
Fragments arranged by size:
1398 32 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 219 1318 1458
Size: 219 1099 140
Fragments arranged by size:
1099 219 140
XmaI C'CCGG_G
Cuts at: 0 16 271 1458
Size: 16 255 1187
Fragments arranged by size:
1187 255 16
Enzymes that do cut:
AccI AcuI AfeI AflII AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BclI Bme1580I
BmrI BpmI Bpu10I BsaBI BsaHI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FspI HaeII Hin4I KasI MmeI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PpuMI
PspOMI PstI PvuII SacI SbfI SexAI SfcI SfoI
SmaI SmlI SphI StyI TaqII TatI TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EcoNI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI ScaI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmnI ZraI