Restriction map for
pFN21AE1149
(Linear) (Six-base) MAPSORT of: KIEE1149.seq Check: 7234 from: 1 to: 1347
KIEE1149.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 241 1347
Size: 241 1106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 932 1037 1347
Size: 519 413 105 310
Fragments arranged by size:
519 413 310 105
AfeI AGC'GCT
Cuts at: 0 940 1347
Size: 940 407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1223 1347
Size: 1223 124
ApaI G_GGCC'C
Cuts at: 0 38 1347
Size: 38 1309
AvaI C'yCGr_G
Cuts at: 0 164 370 1347
Size: 164 206 977
Fragments arranged by size:
977 206 164
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 428 461 1347
Size: 428 33 886
Fragments arranged by size:
886 428 33
BamHI G'GATC_C
Cuts at: 0 57 1347
Size: 57 1290
BanI G'GyrC_C
Cuts at: 0 83 201 294 422 657 923 1347
Size: 83 118 93 128 235 266 424
Fragments arranged by size:
424 266 235 128 118 93 83
BanII G_rGCy'C
Cuts at: 0 38 641 1347
Size: 38 603 706
Fragments arranged by size:
706 603 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 718 1347
Size: 718 629
BbvCI CC'TCA_GC
Cuts at: 0 1061 1347
Size: 1061 286
BglI GCCn_nnn'nGGC
Cuts at: 0 134 287 1347
Size: 134 153 1060
Fragments arranged by size:
1060 153 134
Bme1580I G_kGCm'C
Cuts at: 0 38 139 439 1347
Size: 38 101 300 908
Fragments arranged by size:
908 300 101 38
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 660 1347
Size: 660 687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 274 366 415 634 982 1293 1347
Size: 121 153 92 49 219 348 311 54
Fragments arranged by size:
348 311 219 153 121 92 54 49
Bpu10I CC'TnA_GC
Cuts at: 0 76 1061 1347
Size: 76 985 286
Fragments arranged by size:
985 286 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 406 436 974 1004 1347
Size: 220 30 156 30 538 30 343
Fragments arranged by size:
538 343 220 156 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 424 427 1018 1036 1078 1347
Size: 424 3 591 18 42 269
Fragments arranged by size:
591 424 269 42 18 3
BseYI C'CCAG_C
Cuts at: 0 290 375 941 1347
Size: 290 85 566 406
Fragments arranged by size:
566 406 290 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1347
Size: 196 1151
BsiHKAI G_wGCw'C
Cuts at: 0 392 1347
Size: 392 955
Bsp1286I G_dGCh'C
Cuts at: 0 38 139 392 439 641 1347
Size: 38 101 253 47 202 706
Fragments arranged by size:
706 253 202 101 47 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 241 1347
Size: 241 1106
BsrFI r'CCGG_y
Cuts at: 0 86 128 145 419 1347
Size: 86 42 17 274 928
Fragments arranged by size:
928 274 86 42 17
BssSI C'ACGA_G
Cuts at: 0 319 1347
Size: 319 1028
BstYI r'GATC_y
Cuts at: 0 57 1347
Size: 57 1290
Bsu36I CC'TnA_GG
Cuts at: 0 449 541 1347
Size: 449 92 806
Fragments arranged by size:
806 449 92
BtgI C'CryG_G
Cuts at: 0 22 38 205 455 1347
Size: 22 16 167 250 892
Fragments arranged by size:
892 250 167 22 16
BtsI GCAGTG_nn'
Cuts at: 0 298 1347
Size: 298 1049
DraIII CAC_nnn'GTG
Cuts at: 0 930 1347
Size: 930 417
EaeI y'GGCC_r
Cuts at: 0 143 403 470 1347
Size: 143 260 67 877
Fragments arranged by size:
877 260 143 67
EarI CTCTTCn'nnn_
Cuts at: 0 538 617 971 1013 1109 1347
Size: 538 79 354 42 96 238
Fragments arranged by size:
538 354 238 96 79 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 274 366 415 519 634 932 982
Size: 121 153 92 49 104 115 298 50
Cuts at: 982 1037 1293 1347
Size: 55 256 54
Fragments arranged by size:
298 256 153 121 115 104 92 55
54 50 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 430 1347
Size: 430 917
EcoO109I rG'GnC_Cy
Cuts at: 0 34 330 350 1347
Size: 34 296 20 997
Fragments arranged by size:
997 296 34 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1335 1347
Size: 1335 12
HaeII r_GCGC'y
Cuts at: 0 313 420 942 1347
Size: 313 107 522 405
Fragments arranged by size:
522 405 313 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 1308 1340 1347
Size: 478 32 798 32 7
Fragments arranged by size:
798 478 32 32 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1313 1347
Size: 1313 34
MscI TGG'CCA
Cuts at: 0 405 472 1347
Size: 405 67 875
Fragments arranged by size:
875 405 67
MslI CAynn'nnrTG
Cuts at: 0 868 1347
Size: 868 479
MspA1I CmG'CkG
Cuts at: 0 207 573 859 1170 1271 1347
Size: 207 366 286 311 101 76
Fragments arranged by size:
366 311 286 207 101 76
NaeI GCC'GGC
Cuts at: 0 88 130 147 1347
Size: 88 42 17 1200
Fragments arranged by size:
1200 88 42 17
NdeI CA'TA_TG
Cuts at: 0 812 1347
Size: 812 535
NgoMIV G'CCGG_C
Cuts at: 0 86 128 145 1347
Size: 86 42 17 1202
Fragments arranged by size:
1202 86 42 17
NspI r_CATG'y
Cuts at: 0 1056 1347
Size: 1056 291
PflMI CCAn_nnn'nTGG
Cuts at: 0 573 576 1347
Size: 573 3 771
Fragments arranged by size:
771 573 3
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 1347
Size: 178 32 1137
Fragments arranged by size:
1137 178 32
PspOMI G'GGCC_C
Cuts at: 0 34 1347
Size: 34 1313
PstI C_TGCA'G
Cuts at: 0 278 329 669 1347
Size: 278 51 340 678
Fragments arranged by size:
678 340 278 51
PvuII CAG'CTG
Cuts at: 0 573 859 1170 1271 1347
Size: 573 286 311 101 76
Fragments arranged by size:
573 311 286 101 76
SacII CC_GC'GG
Cuts at: 0 208 1347
Size: 208 1139
SfcI C'TryA_G
Cuts at: 0 274 325 594 665 1347
Size: 274 51 269 71 682
Fragments arranged by size:
682 274 269 71 51
SgrAI Cr'CCGG_yG
Cuts at: 0 419 1347
Size: 419 928
SmaI CCC'GGG
Cuts at: 0 166 1347
Size: 166 1181
SphI G_CATG'C
Cuts at: 0 1056 1347
Size: 1056 291
SspI AAT'ATT
Cuts at: 0 1140 1347
Size: 1140 207
StyI C'CwwG_G
Cuts at: 0 113 1230 1347
Size: 113 1117 117
Fragments arranged by size:
1117 117 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 282 1347
Size: 282 1065
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 406 438 974 1006 1125 1157
Size: 220 32 154 32 536 32 119 32
Cuts at: 1157 1347
Size: 190
Fragments arranged by size:
536 220 190 154 119 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 705 1347
Size: 705 642
XmaI C'CCGG_G
Cuts at: 0 164 1347
Size: 164 1183
Enzymes that do cut:
AarI AcuI AfeI AlfI ApaI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BglI Bme1580I BplI BpmI
Bpu10I BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrFI BssSI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MmeI
MscI MslI MspA1I NaeI NdeI NgoMIV NspI PflMI
PpiI PspOMI PstI PvuII SacII SfcI SgrAI SmaI
SphI SspI StyI TaqII TstI XbaI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NarI NcoI NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SmlI SnaBI SpeI SrfI StuI SwaI TatI
Tth111I XcmI XhoI XmnI ZraI