(Linear) (Six-base) MAPSORT of: KIEE1147.seq  Check: 4384  from: 1  to: 1386



KIEE1147.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:34  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347    1247    1386 
   Size:        347     900     139

  Fragments arranged by size: 

                 900     347     139
 
AlwNI CAG_nnn'CTG

Cuts at:      0     694    1386 
   Size:        694     692
 
ApoI r'AATT_y

Cuts at:      0     173     825    1386 
   Size:        173     652     561

  Fragments arranged by size: 

                 652     561     173
 
AvaI C'yCGr_G

Cuts at:      0     119    1386 
   Size:        119    1267
 
BanII G_rGCy'C

Cuts at:      0     212    1386 
   Size:        212    1174
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     220    1150    1386 
   Size:        220     930     236

  Fragments arranged by size: 

                 930     236     220
 
BbvCI CC'TCA_GC

Cuts at:      0    1284    1386 
   Size:       1284     102
 
Bme1580I G_kGCm'C

Cuts at:      0     630    1386 
   Size:        630     756
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     962    1386 
   Size:        962     424
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     698    1386 
   Size:        698     688
 
Bpu10I CC'TnA_GC

Cuts at:      0    1284    1386 
   Size:       1284     102
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1363    1386 
   Size:       1363      23
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     288     436    1386 
   Size:        288     148     950

  Fragments arranged by size: 

                 950     288     148
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     454    1386 
   Size:        454     932
 
BsmI GAATG_Cn'

Cuts at:      0      60    1386 
   Size:         60    1326
 
Bsp1286I G_dGCh'C

Cuts at:      0     212     630    1386 
   Size:        212     418     756

  Fragments arranged by size: 

                 756     418     212
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     306     969    1386 
   Size:        306     663     417

  Fragments arranged by size: 

                 663     417     306
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     886    1386 
   Size:        886     500
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     471    1204    1386 
   Size:        471     733     182

  Fragments arranged by size: 

                 733     471     182
 
BstYI r'GATC_y

Cuts at:      0     726    1386 
   Size:        726     660
 
BtgI C'CryG_G

Cuts at:      0     464    1386 
   Size:        464     922
 
BtsI GCAGTG_nn'

Cuts at:      0      97    1386 
   Size:         97    1289
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     547     582    1386 
   Size:        547      35     804

  Fragments arranged by size: 

                 804     547      35
 
DraI TTT'AAA

Cuts at:      0    1079    1386 
   Size:       1079     307
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     698    1247    1386 
   Size:        347     351     549     139

  Fragments arranged by size: 

                 549     351     347     139
 
EcoO109I rG'GnC_Cy

Cuts at:      0     255    1231    1386 
   Size:        255     976     155

  Fragments arranged by size: 

                 976     255     155
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     118    1174    1386 
   Size:        118    1056     212

  Fragments arranged by size: 

                1056     212     118
 
MslI CAynn'nnrTG

Cuts at:      0     469    1386 
   Size:        469     917
 
MspA1I CmG'CkG

Cuts at:      0     718    1386 
   Size:        718     668
 
NcoI C'CATG_G

Cuts at:      0     464    1386 
   Size:        464     922
 
PfoI T'CCnGG_A

Cuts at:      0    1182    1386 
   Size:       1182     204
 
PpuMI rG'GwC_Cy

Cuts at:      0     255    1231    1386 
   Size:        255     976     155

  Fragments arranged by size: 

                 976     255     155
 
PstI C_TGCA'G

Cuts at:      0     699    1386 
   Size:        699     687
 
PvuII CAG'CTG

Cuts at:      0     718    1386 
   Size:        718     668
 
ScaI AGT'ACT

Cuts at:      0    1245    1386 
   Size:       1245     141
 
SfcI C'TryA_G

Cuts at:      0     695    1386 
   Size:        695     691
 
SmlI C'TyrA_G

Cuts at:      0    1378    1386 
   Size:       1378       8
 
SpeI A'CTAG_T

Cuts at:      0     859    1386 
   Size:        859     527
 
StyI C'CwwG_G

Cuts at:      0     464    1100    1386 
   Size:        464     636     286

  Fragments arranged by size: 

                 636     464     286
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     183    1386 
   Size:        183    1203
 
TatI w'GTAC_w

Cuts at:      0    1243    1386 
   Size:       1243     143
 
Tth111I GACn'n_nGTC

Cuts at:      0    1141    1386 
   Size:       1141     245
 
XmnI GAAnn'nnTTC

Cuts at:      0     828    1032    1386 
   Size:        828     204     354

  Fragments arranged by size: 

                 828     354     204
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI    BanII     BbsI    BbvCI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BseRI     BsmI Bsp1286I
    BspMI   BstAPI    BstXI    BstYI     BtgI     BtsI    CspCI     DraI
  Eco57MI EcoO109I     FalI     MslI   MspA1I     NcoI     PfoI    PpuMI
     PstI    PvuII     ScaI     SfcI     SmlI     SpeI     StyI    TaqII
     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     ClaI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     SspI     StuI     SwaI     TstI     XbaI     XcmI     XhoI     XmaI
     ZraI