Restriction map for
pFN21AE1144
(Linear) (Six-base) MAPSORT of: KIEE1144.seq Check: 8858 from: 1 to: 1284
KIEE1144.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AclI AA'CG_TT
Cuts at: 0 1068 1284
Size: 1068 216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 392 1284
Size: 103 289 892
Fragments arranged by size:
892 289 103
AflII C'TTAA_G
Cuts at: 0 529 675 1284
Size: 529 146 609
Fragments arranged by size:
609 529 146
AflIII A'CryG_T
Cuts at: 0 744 1284
Size: 744 540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 982 1086 1284
Size: 982 104 198
Fragments arranged by size:
982 198 104
ApoI r'AATT_y
Cuts at: 0 238 895 1077 1284
Size: 238 657 182 207
Fragments arranged by size:
657 238 207 182
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 533 566 1284
Size: 533 33 718
Fragments arranged by size:
718 533 33
BanII G_rGCy'C
Cuts at: 0 171 1284
Size: 171 1113
BbsI GAAGACnn'nnnn_
Cuts at: 0 515 725 1284
Size: 515 210 559
Fragments arranged by size:
559 515 210
BciVI GTATCCnnnnn_n'
Cuts at: 0 655 1284
Size: 655 629
BclI T'GATC_A
Cuts at: 0 293 660 1284
Size: 293 367 624
Fragments arranged by size:
624 367 293
BmrI ACTGGGnnnn_n'
Cuts at: 0 1236 1284
Size: 1236 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 620 1284
Size: 530 90 664
Fragments arranged by size:
664 530 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1284
Size: 583 701
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 23 1284
Size: 23 1261
BseYI C'CCAG_C
Cuts at: 0 827 1284
Size: 827 457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 1284
Size: 470 814
BsmI GAATG_Cn'
Cuts at: 0 87 1284
Size: 87 1197
Bsp1286I G_dGCh'C
Cuts at: 0 171 1284
Size: 171 1113
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 631 933 1284
Size: 631 302 351
Fragments arranged by size:
631 351 302
BsrDI GCAATG_nn'
Cuts at: 0 1058 1284
Size: 1058 226
DraI TTT'AAA
Cuts at: 0 144 416 1284
Size: 144 272 868
Fragments arranged by size:
868 272 144
EaeI y'GGCC_r
Cuts at: 0 578 972 1033 1152 1284
Size: 578 394 61 119 132
Fragments arranged by size:
578 394 132 119 61
EarI CTCTTCn'nnn_
Cuts at: 0 9 161 814 1040 1284
Size: 9 152 653 226 244
Fragments arranged by size:
653 244 226 152 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 392 530 620 1284
Size: 103 289 138 90 664
Fragments arranged by size:
664 289 138 103 90
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 492 985 1284
Size: 492 493 299
Fragments arranged by size:
493 492 299
FspI TGC'GCA
Cuts at: 0 766 1284
Size: 766 518
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 544 576 606 638 1284
Size: 376 32 136 32 30 32 646
Fragments arranged by size:
646 376 136 32 32 32 30
HindIII A'AGCT_T
Cuts at: 0 450 1284
Size: 450 834
MscI TGG'CCA
Cuts at: 0 580 1284
Size: 580 704
MspA1I CmG'CkG
Cuts at: 0 370 620 1284
Size: 370 250 664
Fragments arranged by size:
664 370 250
NspI r_CATG'y
Cuts at: 0 216 748 992 1284
Size: 216 532 244 292
Fragments arranged by size:
532 292 244 216
PciI A'CATG_T
Cuts at: 0 744 1284
Size: 744 540
PfoI T'CCnGG_A
Cuts at: 0 789 1284
Size: 789 495
PstI C_TGCA'G
Cuts at: 0 54 1284
Size: 54 1230
PvuII CAG'CTG
Cuts at: 0 370 620 1284
Size: 370 250 664
Fragments arranged by size:
664 370 250
ScaI AGT'ACT
Cuts at: 0 572 1187 1284
Size: 572 615 97
Fragments arranged by size:
615 572 97
SexAI A'CCwGG_T
Cuts at: 0 200 1284
Size: 200 1084
SfcI C'TryA_G
Cuts at: 0 50 1284
Size: 50 1234
SmlI C'TyrA_G
Cuts at: 0 529 598 675 1284
Size: 529 69 77 609
Fragments arranged by size:
609 529 77 69
SpeI A'CTAG_T
Cuts at: 0 573 1008 1284
Size: 573 435 276
Fragments arranged by size:
573 435 276
SspI AAT'ATT
Cuts at: 0 148 1284
Size: 148 1136
SwaI ATTT'AAAT
Cuts at: 0 144 1284
Size: 144 1140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 867 946 1284
Size: 867 79 338
Fragments arranged by size:
867 338 79
TatI w'GTAC_w
Cuts at: 0 570 938 1185 1284
Size: 570 368 247 99
Fragments arranged by size:
570 368 247 99
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 940 1284
Size: 940 344
Enzymes that do cut:
AclI AcuI AflII AflIII AlfI ApoI BaeI BanII
BbsI BciVI BclI BmrI BpmI BpuEI BseRI BseYI
BsgI BsmI Bsp1286I BspMI BsrDI DraI EaeI EarI
Eco57MI FalI FspI Hin4I HindIII MscI MspA1I NspI
PciI PfoI PstI PvuII ScaI SexAI SfcI SmlI
SpeI SspI SwaI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AgeI AhdI AleI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BanI BbeI BbvCI BcgI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MmeI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI StuI StyI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI