Restriction map for
pFN21AE1142
(Linear) (Six-base) MAPSORT of: KIEE1142.seq Check: 722 from: 1 to: 1260
KIEE1142.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
Acc65I G'GTAC_C
Cuts at: 0 878 1260
Size: 878 382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 751 790 1260
Size: 622 129 39 470
Fragments arranged by size:
622 470 129 39
AflIII A'CryG_T
Cuts at: 0 620 1253 1260
Size: 620 633 7
Fragments arranged by size:
633 620 7
AleI CACnn'nnGTG
Cuts at: 0 290 1260
Size: 290 970
AlwNI CAG_nnn'CTG
Cuts at: 0 231 489 1260
Size: 231 258 771
Fragments arranged by size:
771 258 231
ApaI G_GGCC'C
Cuts at: 0 191 1260
Size: 191 1069
ApoI r'AATT_y
Cuts at: 0 1218 1260
Size: 1218 42
AvaI C'yCGr_G
Cuts at: 0 97 190 1260
Size: 97 93 1070
Fragments arranged by size:
1070 97 93
BanI G'GyrC_C
Cuts at: 0 115 684 878 1260
Size: 115 569 194 382
Fragments arranged by size:
569 382 194 115
BanII G_rGCy'C
Cuts at: 0 52 191 563 1049 1260
Size: 52 139 372 486 211
Fragments arranged by size:
486 372 211 139 52
BbeI G_GCGC'C
Cuts at: 0 119 1260
Size: 119 1141
BbsI GAAGACnn'nnnn_
Cuts at: 0 1123 1260
Size: 1123 137
BbvCI CC'TCA_GC
Cuts at: 0 775 1260
Size: 775 485
BlpI GC'TnA_GC
Cuts at: 0 476 1260
Size: 476 784
Bme1580I G_kGCm'C
Cuts at: 0 97 191 1260
Size: 97 94 1069
Fragments arranged by size:
1069 97 94
BmgBI CAC'GTC
Cuts at: 0 588 1260
Size: 588 672
BmrI ACTGGGnnnn_n'
Cuts at: 0 276 1260
Size: 276 984
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 123 442 1260
Size: 123 319 818
Fragments arranged by size:
818 319 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1126 1230 1260
Size: 1126 104 30
Fragments arranged by size:
1126 104 30
Bpu10I CC'TnA_GC
Cuts at: 0 775 1260
Size: 775 485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1110 1260
Size: 1110 150
BsaAI yAC'GTr
Cuts at: 0 330 615 627 1107 1260
Size: 330 285 12 480 153
Fragments arranged by size:
480 330 285 153 12
BsaHI Gr'CG_yC
Cuts at: 0 116 239 1260
Size: 116 123 1021
Fragments arranged by size:
1021 123 116
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 766 1260
Size: 235 531 494
Fragments arranged by size:
531 494 235
BseYI C'CCAG_C
Cuts at: 0 9 412 433 489 1260
Size: 9 403 21 56 771
Fragments arranged by size:
771 403 56 21 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 1260
Size: 51 1209
BsiEI CG_ry'CG
Cuts at: 0 113 1260
Size: 113 1147
BsiHKAI G_wGCw'C
Cuts at: 0 1049 1073 1260
Size: 1049 24 187
Fragments arranged by size:
1049 187 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 538 1260
Size: 538 722
Bsp1286I G_dGCh'C
Cuts at: 0 52 97 191 563 1049 1073 1260
Size: 52 45 94 372 486 24 187
Fragments arranged by size:
486 372 187 94 52 45 24
BsrFI r'CCGG_y
Cuts at: 0 112 881 973 1260
Size: 112 769 92 287
Fragments arranged by size:
769 287 112 92
BsrGI T'GTAC_A
Cuts at: 0 338 1260
Size: 338 922
BssHII G'CGCG_C
Cuts at: 0 606 1260
Size: 606 654
BstAPI GCAn_nnn'nTGC
Cuts at: 0 74 231 1260
Size: 74 157 1029
Fragments arranged by size:
1029 157 74
BstYI r'GATC_y
Cuts at: 0 860 1241 1260
Size: 860 381 19
Fragments arranged by size:
860 381 19
BtgI C'CryG_G
Cuts at: 0 144 345 1260
Size: 144 201 915
Fragments arranged by size:
915 201 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 648 1260
Size: 648 612
DraI TTT'AAA
Cuts at: 0 1085 1260
Size: 1085 175
DraIII CAC_nnn'GTG
Cuts at: 0 74 1260
Size: 74 1186
EaeI y'GGCC_r
Cuts at: 0 38 110 126 1260
Size: 38 72 16 1134
Fragments arranged by size:
1134 72 38 16
EagI C'GGCC_G
Cuts at: 0 110 1260
Size: 110 1150
EarI CTCTTCn'nnn_
Cuts at: 0 902 943 1260
Size: 902 41 317
Fragments arranged by size:
902 317 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 669 1260
Size: 19 650 591
Fragments arranged by size:
650 591 19
EcoICRI GAG'CTC
Cuts at: 0 1047 1260
Size: 1047 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 751 790 1126 1230 1260
Size: 622 129 39 336 104 30
Fragments arranged by size:
622 336 129 104 39 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 40 358 700 1260
Size: 40 318 342 560
Fragments arranged by size:
560 342 318 40
EcoO109I rG'GnC_Cy
Cuts at: 0 187 421 1260
Size: 187 234 839
Fragments arranged by size:
839 234 187
HaeII r_GCGC'y
Cuts at: 0 119 174 730 1260
Size: 119 55 556 530
Fragments arranged by size:
556 530 119 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1036 1068 1260
Size: 1036 32 192
Fragments arranged by size:
1036 192 32
HincII GTy'rAC
Cuts at: 0 570 1260
Size: 570 690
KasI G'GCGC_C
Cuts at: 0 115 1260
Size: 115 1145
KpnI G_GTAC'C
Cuts at: 0 882 1260
Size: 882 378
MluI A'CGCG_T
Cuts at: 0 1253 1260
Size: 1253 7
MslI CAynn'nnrTG
Cuts at: 0 290 469 625 901 1260
Size: 290 179 156 276 359
Fragments arranged by size:
359 290 276 179 156
MspA1I CmG'CkG
Cuts at: 0 54 65 156 228 416 450 459 489
Size: 54 11 91 72 188 34 9 30
Cuts at: 489 504 557 651 783 876 1260
Size: 15 53 94 132 93 384
Fragments arranged by size:
384 188 132 94 93 91 72 54
53 34 30 15 11 9
NaeI GCC'GGC
Cuts at: 0 114 975 1260
Size: 114 861 285
Fragments arranged by size:
861 285 114
NarI GG'CG_CC
Cuts at: 0 116 1260
Size: 116 1144
NgoMIV G'CCGG_C
Cuts at: 0 112 973 1260
Size: 112 861 287
Fragments arranged by size:
861 287 112
NruI TCG'CGA
Cuts at: 0 218 1061 1260
Size: 218 843 199
Fragments arranged by size:
843 218 199
NspI r_CATG'y
Cuts at: 0 339 501 624 834 1260
Size: 339 162 123 210 426
Fragments arranged by size:
426 339 210 162 123
PciI A'CATG_T
Cuts at: 0 620 1260
Size: 620 640
PflMI CCAn_nnn'nTGG
Cuts at: 0 159 1260
Size: 159 1101
PfoI T'CCnGG_A
Cuts at: 0 298 1260
Size: 298 962
PmlI CAC'GTG
Cuts at: 0 615 1260
Size: 615 645
PsiI TTA'TAA
Cuts at: 0 1126 1260
Size: 1126 134
PspOMI G'GGCC_C
Cuts at: 0 187 1260
Size: 187 1073
PstI C_TGCA'G
Cuts at: 0 875 1078 1260
Size: 875 203 182
Fragments arranged by size:
875 203 182
PvuII CAG'CTG
Cuts at: 0 156 228 489 1260
Size: 156 72 261 771
Fragments arranged by size:
771 261 156 72
SacI G_AGCT'C
Cuts at: 0 1049 1260
Size: 1049 211
ScaI AGT'ACT
Cuts at: 0 22 1260
Size: 22 1238
SfcI C'TryA_G
Cuts at: 0 871 1074 1260
Size: 871 203 186
Fragments arranged by size:
871 203 186
SfoI GGC'GCC
Cuts at: 0 117 1260
Size: 117 1143
SmlI C'TyrA_G
Cuts at: 0 1089 1260
Size: 1089 171
StuI AGG'CCT
Cuts at: 0 364 1260
Size: 364 896
StyI C'CwwG_G
Cuts at: 0 746 1260
Size: 746 514
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 447 1260
Size: 447 813
TatI w'GTAC_w
Cuts at: 0 20 29 338 1260
Size: 20 9 309 922
Fragments arranged by size:
922 309 20 9
Tth111I GACn'n_nGTC
Cuts at: 0 1195 1260
Size: 1195 65
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BsrGI
BssHII BstAPI BstYI BtgI BtgZI DraI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII KasI KpnI MluI MslI MspA1I NaeI
NarI NgoMIV NruI NspI PciI PflMI PfoI PmlI
PsiI PspOMI PstI PvuII SacI ScaI SfcI SfoI
SmlI StuI StyI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglI BglII BmtI
BsaI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI
BspMI BsrBI BsrDI BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DrdI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI MfeI MmeI MscI
NcoI NdeI NheI NotI NsiI PacI PasI PmeI
PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XcmI XhoI XmaI XmnI ZraI