Restriction map for
pFN21AE1139
(Linear) (Six-base) MAPSORT of: KIEE1139.seq Check: 4364 from: 1 to: 1191
KIEE1139.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AccI GT'mk_AC
Cuts at: 0 880 1191
Size: 880 311
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 289 1191
Size: 130 159 902
Fragments arranged by size:
902 159 130
AlwNI CAG_nnn'CTG
Cuts at: 0 87 108 444 1191
Size: 87 21 336 747
Fragments arranged by size:
747 336 87 21
ApoI r'AATT_y
Cuts at: 0 214 1191
Size: 214 977
AvaI C'yCGr_G
Cuts at: 0 1081 1191
Size: 1081 110
AvrII C'CTAG_G
Cuts at: 0 345 1191
Size: 345 846
BamHI G'GATC_C
Cuts at: 0 154 239 1191
Size: 154 85 952
Fragments arranged by size:
952 154 85
BanII G_rGCy'C
Cuts at: 0 320 692 899 1191
Size: 320 372 207 292
Fragments arranged by size:
372 320 292 207
BbsI GAAGACnn'nnnn_
Cuts at: 0 290 749 1191
Size: 290 459 442
Fragments arranged by size:
459 442 290
BciVI GTATCCnnnnn_n'
Cuts at: 0 543 940 1191
Size: 543 397 251
Fragments arranged by size:
543 397 251
BfrBI ATG'CAT
Cuts at: 0 385 1191
Size: 385 806
BmrI ACTGGGnnnn_n'
Cuts at: 0 20 288 1191
Size: 20 268 903
Fragments arranged by size:
903 268 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1191
Size: 79 1112
BsaI GGTCTCn'nnnn_
Cuts at: 0 668 1176 1191
Size: 668 508 15
Fragments arranged by size:
668 508 15
BsaBI GATnn'nnATC
Cuts at: 0 416 1191
Size: 416 775
BsaHI Gr'CG_yC
Cuts at: 0 1078 1191
Size: 1078 113
BsaWI w'CCGG_w
Cuts at: 0 485 695 1061 1191
Size: 485 210 366 130
Fragments arranged by size:
485 366 210 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 580 1191
Size: 550 30 611
Fragments arranged by size:
611 550 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 58 682 700 1099 1108 1164 1191
Size: 19 39 624 18 399 9 56 27
Fragments arranged by size:
624 399 56 39 27 19 18 9
BseYI C'CCAG_C
Cuts at: 0 506 1191
Size: 506 685
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1191
Size: 583 608
BsiEI CG_ry'CG
Cuts at: 0 486 1191
Size: 486 705
BsiHKAI G_wGCw'C
Cuts at: 0 692 1191
Size: 692 499
BsmBI CGTCTCn'nnnn_
Cuts at: 0 828 1191
Size: 828 363
Bsp1286I G_dGCh'C
Cuts at: 0 320 692 899 1191
Size: 320 372 207 292
Fragments arranged by size:
372 320 292 207
BspEI T'CCGG_A
Cuts at: 0 695 1191
Size: 695 496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 686 1191
Size: 686 505
BsrDI GCAATG_nn'
Cuts at: 0 287 641 1191
Size: 287 354 550
Fragments arranged by size:
550 354 287
BstYI r'GATC_y
Cuts at: 0 154 239 1064 1191
Size: 154 85 825 127
Fragments arranged by size:
825 154 127 85
BstZ17I GTA'TAC
Cuts at: 0 881 1191
Size: 881 310
BtgI C'CryG_G
Cuts at: 0 200 545 884 900 1191
Size: 200 345 339 16 291
Fragments arranged by size:
345 339 291 200 16
DraI TTT'AAA
Cuts at: 0 732 1191
Size: 732 459
EarI CTCTTCn'nnn_
Cuts at: 0 826 1191
Size: 826 365
EcoICRI GAG'CTC
Cuts at: 0 690 1191
Size: 690 501
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 130 289 1191
Size: 79 51 159 902
Fragments arranged by size:
902 159 79 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 682 1191
Size: 682 509
EcoO109I rG'GnC_Cy
Cuts at: 0 1131 1191
Size: 1131 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1056 1088 1191
Size: 1056 32 103
Fragments arranged by size:
1056 103 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 1099 1191
Size: 93 1006 92
Fragments arranged by size:
1006 93 92
MslI CAynn'nnrTG
Cuts at: 0 580 646 1191
Size: 580 66 545
Fragments arranged by size:
580 545 66
NcoI C'CATG_G
Cuts at: 0 200 1191
Size: 200 991
NsiI A_TGCA'T
Cuts at: 0 387 1191
Size: 387 804
NspI r_CATG'y
Cuts at: 0 180 1191
Size: 180 1011
PfoI T'CCnGG_A
Cuts at: 0 932 1191
Size: 932 259
PpuMI rG'GwC_Cy
Cuts at: 0 1131 1191
Size: 1131 60
SacI G_AGCT'C
Cuts at: 0 692 1191
Size: 692 499
SanDI GG'GwC_CC
Cuts at: 0 1131 1191
Size: 1131 60
StuI AGG'CCT
Cuts at: 0 1009 1191
Size: 1009 182
StyI C'CwwG_G
Cuts at: 0 200 345 1191
Size: 200 145 846
Fragments arranged by size:
846 200 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 800 1033 1191
Size: 800 233 158
Fragments arranged by size:
800 233 158
Enzymes that do cut:
AccI AcuI AlwNI ApoI AvaI AvrII BamHI BanII
BbsI BciVI BfrBI BmrI BpmI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrDI BstYI BstZ17I BtgI DraI
EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MmeI MslI
NcoI NsiI NspI PfoI PpuMI SacI SanDI StuI
StyI TaqII
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI BaeI BanI BbeI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI
BsaAI BsiWI BsmI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI