Restriction map for
pFN21AE1130
(Linear) (Six-base) MAPSORT of: KIEE1130.seq Check: 65 from: 1 to: 663
KIEE1130.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 110 663
Size: 110 553
AccI GT'mk_AC
Cuts at: 0 497 663
Size: 497 166
AflIII A'CryG_T
Cuts at: 0 212 663
Size: 212 451
AlwNI CAG_nnn'CTG
Cuts at: 0 561 663
Size: 561 102
ApoI r'AATT_y
Cuts at: 0 358 663
Size: 358 305
AvaI C'yCGr_G
Cuts at: 0 30 546 663
Size: 30 516 117
Fragments arranged by size:
516 117 30
BanI G'GyrC_C
Cuts at: 0 40 663
Size: 40 623
BbeI G_GCGC'C
Cuts at: 0 44 663
Size: 44 619
BbsI GAAGACnn'nnnn_
Cuts at: 0 594 663
Size: 594 69
BciVI GTATCCnnnnn_n'
Cuts at: 0 484 663
Size: 484 179
BclI T'GATC_A
Cuts at: 0 350 663
Size: 350 313
BmrI ACTGGGnnnn_n'
Cuts at: 0 116 663
Size: 116 547
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 479 663
Size: 479 184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 663
Size: 106 557
BsaI GGTCTCn'nnnn_
Cuts at: 0 624 663
Size: 624 39
BsaHI Gr'CG_yC
Cuts at: 0 41 256 663
Size: 41 215 407
Fragments arranged by size:
407 215 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 663
Size: 91 30 542
Fragments arranged by size:
542 91 30
BseYI C'CCAG_C
Cuts at: 0 53 663
Size: 53 610
BsiEI CG_ry'CG
Cuts at: 0 501 663
Size: 501 162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 663
Size: 110 553
BsrDI GCAATG_nn'
Cuts at: 0 447 663
Size: 447 216
BsrFI r'CCGG_y
Cuts at: 0 500 663
Size: 500 163
BsrGI T'GTAC_A
Cuts at: 0 628 663
Size: 628 35
BssHII G'CGCG_C
Cuts at: 0 151 153 663
Size: 151 2 510
Fragments arranged by size:
510 151 2
BstEII G'GTnAC_C
Cuts at: 0 97 330 663
Size: 97 233 333
Fragments arranged by size:
333 233 97
Bsu36I CC'TnA_GG
Cuts at: 0 337 663
Size: 337 326
BtsI GCAGTG_nn'
Cuts at: 0 73 663
Size: 73 590
EarI CTCTTCn'nnn_
Cuts at: 0 317 422 663
Size: 317 105 241
Fragments arranged by size:
317 241 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 99 663
Size: 99 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 663
Size: 106 557
EcoO109I rG'GnC_Cy
Cuts at: 0 90 184 663
Size: 90 94 479
Fragments arranged by size:
479 94 90
EcoRI G'AATT_C
Cuts at: 0 358 663
Size: 358 305
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 507 663
Size: 507 156
HaeII r_GCGC'y
Cuts at: 0 44 562 663
Size: 44 518 101
Fragments arranged by size:
518 101 44
HincII GTy'rAC
Cuts at: 0 498 663
Size: 498 165
KasI G'GCGC_C
Cuts at: 0 40 663
Size: 40 623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 416 663
Size: 116 300 247
Fragments arranged by size:
300 247 116
MslI CAynn'nnrTG
Cuts at: 0 580 663
Size: 580 83
MspA1I CmG'CkG
Cuts at: 0 144 663
Size: 144 519
NarI GG'CG_CC
Cuts at: 0 41 663
Size: 41 622
NdeI CA'TA_TG
Cuts at: 0 381 663
Size: 381 282
PasI CC'CwG_GG
Cuts at: 0 93 663
Size: 93 570
PfoI T'CCnGG_A
Cuts at: 0 194 663
Size: 194 469
PstI C_TGCA'G
Cuts at: 0 68 149 524 662 663
Size: 68 81 375 138 1
Fragments arranged by size:
375 138 81 68 1
PvuII CAG'CTG
Cuts at: 0 144 663
Size: 144 519
SalI G'TCGA_C
Cuts at: 0 496 663
Size: 496 167
SapI GCTCTTCn'nnn_
Cuts at: 0 317 663
Size: 317 346
ScaI AGT'ACT
Cuts at: 0 487 663
Size: 487 176
SfcI C'TryA_G
Cuts at: 0 64 145 520 658 663
Size: 64 81 375 138 5
Fragments arranged by size:
375 138 81 64 5
SfoI GGC'GCC
Cuts at: 0 42 663
Size: 42 621
StyI C'CwwG_G
Cuts at: 0 77 663
Size: 77 586
TatI w'GTAC_w
Cuts at: 0 485 628 663
Size: 485 143 35
Fragments arranged by size:
485 143 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 91 123 663
Size: 91 32 540
Fragments arranged by size:
540 91 32
Enzymes that do cut:
AarI AccI AflIII AlwNI ApoI AvaI BanI BbeI
BbsI BciVI BclI BmrI BplI BpmI BsaI BsaHI
BsaXI BseYI BsiEI BspMI BsrDI BsrFI BsrGI BssHII
BstEII Bsu36I BtsI EarI EciI Eco57MI EcoO109I EcoRI
FalI HaeII HincII KasI MmeI MslI MspA1I NarI
NdeI PasI PfoI PstI PvuII SalI SapI ScaI
SfcI SfoI StyI TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbvCI BcgI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI BsaAI
BsaBI BsaWI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BsrBI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EcoICRI EcoNI EcoRV FseI FspI
FspAI Hin4I HindIII HpaI KpnI MfeI MluI MscI
NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI