Restriction map for
pFN21AE1129
(Linear) (Six-base) MAPSORT of: KIEE1129.seq Check: 9645 from: 1 to: 498
KIEE1129.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 498
Size: 478 20
AflIII A'CryG_T
Cuts at: 0 367 498
Size: 367 131
AleI CACnn'nnGTG
Cuts at: 0 370 498
Size: 370 128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 249 498
Size: 249 249
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 498
Size: 82 32 384
Fragments arranged by size:
384 82 32
ApaI G_GGCC'C
Cuts at: 0 423 498
Size: 423 75
ApaLI G'TGCA_C
Cuts at: 0 254 498
Size: 254 244
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 237 270 498
Size: 237 33 228
Fragments arranged by size:
237 228 33
BanI G'GyrC_C
Cuts at: 0 19 413 498
Size: 19 394 85
Fragments arranged by size:
394 85 19
BanII G_rGCy'C
Cuts at: 0 44 423 498
Size: 44 379 75
Fragments arranged by size:
379 75 44
BbeI G_GCGC'C
Cuts at: 0 23 498
Size: 23 475
BglI GCCn_nnn'nGGC
Cuts at: 0 289 498
Size: 289 209
Bme1580I G_kGCm'C
Cuts at: 0 258 423 498
Size: 258 165 75
Fragments arranged by size:
258 165 75
BmgBI CAC'GTC
Cuts at: 0 98 498
Size: 98 400
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 40 498
Size: 40 458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 498
Size: 66 432
BsaI GGTCTCn'nnnn_
Cuts at: 0 339 498
Size: 339 159
BsaHI Gr'CG_yC
Cuts at: 0 20 498
Size: 20 478
BseYI C'CCAG_C
Cuts at: 0 445 498
Size: 445 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 229 498
Size: 131 98 269
Fragments arranged by size:
269 131 98
BsiHKAI G_wGCw'C
Cuts at: 0 44 258 498
Size: 44 214 240
Fragments arranged by size:
240 214 44
Bsp1286I G_dGCh'C
Cuts at: 0 44 258 423 498
Size: 44 214 165 75
Fragments arranged by size:
214 165 75 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 188 202 498
Size: 188 14 296
Fragments arranged by size:
296 188 14
BsrFI r'CCGG_y
Cuts at: 0 431 498
Size: 431 67
BssSI C'ACGA_G
Cuts at: 0 436 498
Size: 436 62
BtgI C'CryG_G
Cuts at: 0 122 186 284 498
Size: 122 64 98 214
Fragments arranged by size:
214 122 98 64
BtsI GCAGTG_nn'
Cuts at: 0 140 381 498
Size: 140 241 117
Fragments arranged by size:
241 140 117
DraIII CAC_nnn'GTG
Cuts at: 0 262 498
Size: 262 236
EaeI y'GGCC_r
Cuts at: 0 281 498
Size: 281 217
EcoICRI GAG'CTC
Cuts at: 0 42 498
Size: 42 456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 498
Size: 478 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 457 498
Size: 457 41
EcoO109I rG'GnC_Cy
Cuts at: 0 420 498
Size: 420 78
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 488 498
Size: 488 10
HaeII r_GCGC'y
Cuts at: 0 23 452 498
Size: 23 429 46
Fragments arranged by size:
429 46 23
KasI G'GCGC_C
Cuts at: 0 19 498
Size: 19 479
MslI CAynn'nnrTG
Cuts at: 0 149 370 498
Size: 149 221 128
Fragments arranged by size:
221 149 128
MspA1I CmG'CkG
Cuts at: 0 105 498
Size: 105 393
NaeI GCC'GGC
Cuts at: 0 433 498
Size: 433 65
NarI GG'CG_CC
Cuts at: 0 20 498
Size: 20 478
NcoI C'CATG_G
Cuts at: 0 186 498
Size: 186 312
NgoMIV G'CCGG_C
Cuts at: 0 431 498
Size: 431 67
NspI r_CATG'y
Cuts at: 0 230 271 371 498
Size: 230 41 100 127
Fragments arranged by size:
230 127 100 41
PciI A'CATG_T
Cuts at: 0 367 498
Size: 367 131
PflMI CCAn_nnn'nTGG
Cuts at: 0 84 498
Size: 84 414
PspOMI G'GGCC_C
Cuts at: 0 419 498
Size: 419 79
PstI C_TGCA'G
Cuts at: 0 132 199 498
Size: 132 67 299
Fragments arranged by size:
299 132 67
SacI G_AGCT'C
Cuts at: 0 44 498
Size: 44 454
SbfI CC_TGCA'GG
Cuts at: 0 199 498
Size: 199 299
SfcI C'TryA_G
Cuts at: 0 128 195 498
Size: 128 67 303
Fragments arranged by size:
303 128 67
SfoI GGC'GCC
Cuts at: 0 21 498
Size: 21 477
SmlI C'TyrA_G
Cuts at: 0 45 498
Size: 45 453
SphI G_CATG'C
Cuts at: 0 271 498
Size: 271 227
StuI AGG'CCT
Cuts at: 0 82 498
Size: 82 416
StyI C'CwwG_G
Cuts at: 0 83 186 323 498
Size: 83 103 137 175
Fragments arranged by size:
175 137 103 83
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 345 498
Size: 321 24 153
Fragments arranged by size:
321 153 24
Enzymes that do cut:
AcuI AflIII AleI AlfI AloI ApaI ApaLI BaeI
BanI BanII BbeI BglI Bme1580I BmgBI BplI BpuEI
BsaI BsaHI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI
BssSI BtgI BtsI DraIII EaeI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII KasI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PspOMI PstI SacI
SbfI SfcI SfoI SmlI SphI StuI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlwNI ApoI AscI AseI AsiSI AvaI AvrII
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BpmI Bpu10I BsaAI BsaBI BsaWI
BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EagI EarI EciI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI