(Linear) (Six-base) MAPSORT of: KIEE1128.seq  Check: 3872  from: 1  to: 495



KIEE1128.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     148     495 
   Size:        148     347
 
ApaI G_GGCC'C

Cuts at:      0     130     495 
   Size:        130     365
 
BanII G_rGCy'C

Cuts at:      0     130     389     495 
   Size:        130     259     106

  Fragments arranged by size: 

                 259     130     106
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     140     495 
   Size:        140     355
 
BclI T'GATC_A

Cuts at:      0     119     495 
   Size:        119     376
 
Bme1580I G_kGCm'C

Cuts at:      0     130     495 
   Size:        130     365
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       1     495 
   Size:          1     494
 
BsaWI w'CCGG_w

Cuts at:      0      60     495 
   Size:         60     435
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     395     425     495 
   Size:        395      30      70

  Fragments arranged by size: 

                 395      70      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     495 
   Size:        358     137
 
Bsp1286I G_dGCh'C

Cuts at:      0     130     389     495 
   Size:        130     259     106

  Fragments arranged by size: 

                 259     130     106
 
BspEI T'CCGG_A

Cuts at:      0      60     495 
   Size:         60     435
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     148     495 
   Size:        148     347
 
BsrBI CCG'CTC

Cuts at:      0     324     495 
   Size:        324     171
 
BstYI r'GATC_y

Cuts at:      0     353     495 
   Size:        353     142
 
BtgI C'CryG_G

Cuts at:      0     299     495 
   Size:        299     196
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     305     449     495 
   Size:        305     144      46

  Fragments arranged by size: 

                 305     144      46
 
EaeI y'GGCC_r

Cuts at:      0      29     495 
   Size:         29     466
 
EarI CTCTTCn'nnn_

Cuts at:      0      86     495 
   Size:         86     409
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      62     495 
   Size:         62     433
 
EcoO109I rG'GnC_Cy

Cuts at:      0      79     495 
   Size:         79     416
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     219     251     495 
   Size:        219      32     244

  Fragments arranged by size: 

                 244     219      32
 
NcoI C'CATG_G

Cuts at:      0     299     495 
   Size:        299     196
 
NspI r_CATG'y

Cuts at:      0     378     495 
   Size:        378     117
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     428     495 
   Size:        428      67
 
PspOMI G'GGCC_C

Cuts at:      0     126     495 
   Size:        126     369
 
SmlI C'TyrA_G

Cuts at:      0      16     495 
   Size:         16     479
 
SphI G_CATG'C

Cuts at:      0     378     495 
   Size:        378     117
 
StyI C'CwwG_G

Cuts at:      0     299     495 
   Size:        299     196
 
TatI w'GTAC_w

Cuts at:      0      35     495 
   Size:         35     460
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     395     427     495 
   Size:        395      32      68

  Fragments arranged by size: 

                 395      68      32
 

 Enzymes that do cut:

     AarI     ApaI    BanII     BbsI     BclI Bme1580I    BpuEI    BsaWI
    BsaXI     BsgI Bsp1286I    BspEI    BspMI    BsrBI    BstYI     BtgI
    BtgZI     EaeI     EarI    EcoNI EcoO109I    Hin4I     NcoI     NspI
    PflMI   PspOMI     SmlI     SphI     StyI     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI