(Linear) (Six-base) MAPSORT of: KIEE1127.seq  Check: 2910  from: 1  to: 354



KIEE1127.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     101     354 
   Size:        101     253
 
AvaI C'yCGr_G

Cuts at:      0     297     354 
   Size:        297      57
 
BamHI G'GATC_C

Cuts at:      0     281     354 
   Size:        281      73
 
BanI G'GyrC_C

Cuts at:      0     324     354 
   Size:        324      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     157     354 
   Size:        157     197
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     262     354 
   Size:        199      63      92

  Fragments arranged by size: 

                 199      92      63
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     172     202     354 
   Size:        172      30     152

  Fragments arranged by size: 

                 172     152      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     354 
   Size:         19     335
 
BseYI C'CCAG_C

Cuts at:      0     198     354 
   Size:        198     156
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     354 
   Size:        247     107
 
BsiEI CG_ry'CG

Cuts at:      0      14     354 
   Size:         14     340
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     101     266     354 
   Size:        101     165      88

  Fragments arranged by size: 

                 165     101      88
 
BstYI r'GATC_y

Cuts at:      0     281     354 
   Size:        281      73
 
Bsu36I CC'TnA_GG

Cuts at:      0     320     354 
   Size:        320      34
 
BtgI C'CryG_G

Cuts at:      0      31      59     158     354 
   Size:         31      28      99     196

  Fragments arranged by size: 

                 196      99      31      28
 
EaeI y'GGCC_r

Cuts at:      0      11     122     354 
   Size:         11     111     232

  Fragments arranged by size: 

                 232     111      11
 
EagI C'GGCC_G

Cuts at:      0      11     354 
   Size:         11     343
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     262     354 
   Size:        199      63      92

  Fragments arranged by size: 

                 199      92      63
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     211     354 
   Size:        211     143
 
EcoO109I rG'GnC_Cy

Cuts at:      0     203     354 
   Size:        203     151
 
MspA1I CmG'CkG

Cuts at:      0     177     354 
   Size:        177     177
 
NcoI C'CATG_G

Cuts at:      0      59     354 
   Size:         59     295
 
NotI GC'GGCC_GC

Cuts at:      0      11     354 
   Size:         11     343
 
PpuMI rG'GwC_Cy

Cuts at:      0     203     354 
   Size:        203     151
 
PstI C_TGCA'G

Cuts at:      0     215     233     354 
   Size:        215      18     121

  Fragments arranged by size: 

                 215     121      18
 
PvuII CAG'CTG

Cuts at:      0     177     354 
   Size:        177     177
 
SanDI GG'GwC_CC

Cuts at:      0     203     354 
   Size:        203     151
 
SfcI C'TryA_G

Cuts at:      0     211     229     354 
   Size:        211      18     125

  Fragments arranged by size: 

                 211     125      18
 
SmaI CCC'GGG

Cuts at:      0     299     354 
   Size:        299      55
 
StyI C'CwwG_G

Cuts at:      0      59     354 
   Size:         59     295
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      82     114     172     204     354 
   Size:         82      32      58      32     150

  Fragments arranged by size: 

                 150      82      58      32      32
 
XmaI C'CCGG_G

Cuts at:      0     297     354 
   Size:        297      57
 

 Enzymes that do cut:

     AarI     AvaI    BamHI     BanI     BbsI     BpmI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BspMI    BstYI   Bsu36I     BtgI     EaeI
     EagI  Eco57MI    EcoNI EcoO109I   MspA1I     NcoI     NotI    PpuMI
     PstI    PvuII    SanDI     SfcI     SmaI     StyI     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EarI     EciI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI