Restriction map for
pFN21AE1126
(Linear) (Six-base) MAPSORT of: KIEE1126.seq Check: 1730 from: 1 to: 1893
KIEE1126.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 632 1893
Size: 632 1261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 496 553 868 1893
Size: 225 271 57 315 1025
Fragments arranged by size:
1025 315 271 225 57
AflIII A'CryG_T
Cuts at: 0 385 1893
Size: 385 1508
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1474 1506 1893
Size: 1474 32 387
Fragments arranged by size:
1474 387 32
AlwNI CAG_nnn'CTG
Cuts at: 0 846 936 1041 1717 1735 1893
Size: 846 90 105 676 18 158
Fragments arranged by size:
846 676 158 105 90 18
ApaI G_GGCC'C
Cuts at: 0 137 1328 1893
Size: 137 1191 565
Fragments arranged by size:
1191 565 137
AscI GG'CGCG_CC
Cuts at: 0 1233 1893
Size: 1233 660
AvaI C'yCGr_G
Cuts at: 0 152 258 1893
Size: 152 106 1635
Fragments arranged by size:
1635 152 106
BanI G'GyrC_C
Cuts at: 0 147 649 921 1893
Size: 147 502 272 972
Fragments arranged by size:
972 502 272 147
BanII G_rGCy'C
Cuts at: 0 137 161 760 817 1062 1328 1673 1877
Size: 137 24 599 57 245 266 345 204
Cuts at: 1877 1893
Size: 16
Fragments arranged by size:
599 345 266 245 204 137 57 24
16
BbeI G_GCGC'C
Cuts at: 0 151 653 1893
Size: 151 502 1240
Fragments arranged by size:
1240 502 151
BbvCI CC'TCA_GC
Cuts at: 0 829 1893
Size: 829 1064
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1046 1080 1893
Size: 1046 34 813
Fragments arranged by size:
1046 813 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 846 1893
Size: 846 1047
BglI GCCn_nnn'nGGC
Cuts at: 0 357 1513 1765 1774 1893
Size: 357 1156 252 9 119
Fragments arranged by size:
1156 357 252 119 9
BlpI GC'TnA_GC
Cuts at: 0 766 811 1358 1577 1893
Size: 766 45 547 219 316
Fragments arranged by size:
766 547 316 219 45
Bme1580I G_kGCm'C
Cuts at: 0 137 1328 1893
Size: 137 1191 565
Fragments arranged by size:
1191 565 137
BmgBI CAC'GTC
Cuts at: 0 386 1893
Size: 386 1507
BmrI ACTGGGnnnn_n'
Cuts at: 0 496 1893
Size: 496 1397
BmtI G_CTAG'C
Cuts at: 0 1282 1893
Size: 1282 611
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 552 1893
Size: 552 1341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 607 892 1772 1867 1893
Size: 202 405 285 880 95 26
Fragments arranged by size:
880 405 285 202 95 26
Bpu10I CC'TnA_GC
Cuts at: 0 761 829 1283 1893
Size: 761 68 454 610
Fragments arranged by size:
761 610 454 68
BsaAI yAC'GTr
Cuts at: 0 888 1893
Size: 888 1005
BsaHI Gr'CG_yC
Cuts at: 0 148 650 1257 1893
Size: 148 502 607 636
Fragments arranged by size:
636 607 502 148
BsaWI w'CCGG_w
Cuts at: 0 320 1341 1893
Size: 320 1021 552
Fragments arranged by size:
1021 552 320
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 239 269 1474 1504 1745 1775 1893
Size: 239 30 1205 30 241 30 118
Fragments arranged by size:
1205 241 239 118 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 502 544 629 632 1420 1477 1855 1893
Size: 502 42 85 3 788 57 378 38
Fragments arranged by size:
788 502 378 85 57 42 38 3
BseYI C'CCAG_C
Cuts at: 0 137 699 1190 1735 1804 1893
Size: 137 562 491 545 69 89
Fragments arranged by size:
562 545 491 137 89 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 1054 1348 1703 1893
Size: 356 698 294 355 190
Fragments arranged by size:
698 356 355 294 190
BsiEI CG_ry'CG
Cuts at: 0 361 606 1893
Size: 361 245 1287
Fragments arranged by size:
1287 361 245
BsiHKAI G_wGCw'C
Cuts at: 0 161 798 817 1893
Size: 161 637 19 1076
Fragments arranged by size:
1076 637 161 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 259 376 1170 1893
Size: 259 117 794 723
Fragments arranged by size:
794 723 259 117
Bsp1286I G_dGCh'C
Cuts at: 0 137 161 760 798 817 1062 1328 1673
Size: 137 24 599 38 19 245 266 345
Cuts at: 1673 1877 1893
Size: 204 16
Fragments arranged by size:
599 345 266 245 204 137 38 24
19 16
BspEI T'CCGG_A
Cuts at: 0 320 1341 1893
Size: 320 1021 552
Fragments arranged by size:
1021 552 320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 175 632 1893
Size: 175 457 1261
Fragments arranged by size:
1261 457 175
BsrFI r'CCGG_y
Cuts at: 0 605 1893
Size: 605 1288
BssHII G'CGCG_C
Cuts at: 0 1233 1893
Size: 1233 660
BstAPI GCAn_nnn'nTGC
Cuts at: 0 236 1041 1717 1726 1893
Size: 236 805 676 9 167
Fragments arranged by size:
805 676 236 167 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 180 207 1893
Size: 180 27 1686
Fragments arranged by size:
1686 180 27
BstYI r'GATC_y
Cuts at: 0 824 1893
Size: 824 1069
Bsu36I CC'TnA_GG
Cuts at: 0 448 1372 1893
Size: 448 924 521
Fragments arranged by size:
924 521 448
BtgI C'CryG_G
Cuts at: 0 143 1238 1521 1589 1760 1893
Size: 143 1095 283 68 171 133
Fragments arranged by size:
1095 283 171 143 133 68
BtsI GCAGTG_nn'
Cuts at: 0 373 1893
Size: 373 1520
DrdI GACnn_nn'nnGTC
Cuts at: 0 502 1893
Size: 502 1391
EaeI y'GGCC_r
Cuts at: 0 216 358 488 603 607 890 1241 1592
Size: 216 142 130 115 4 283 351 351
Cuts at: 1592 1632 1893
Size: 40 261
Fragments arranged by size:
351 351 283 261 216 142 130 115
40 4
EagI C'GGCC_G
Cuts at: 0 358 603 1893
Size: 358 245 1290
Fragments arranged by size:
1290 358 245
EarI CTCTTCn'nnn_
Cuts at: 0 304 1883 1893
Size: 304 1579 10
Fragments arranged by size:
1579 304 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 415 1110 1132 1893
Size: 415 695 22 761
Fragments arranged by size:
761 695 415 22
EcoICRI GAG'CTC
Cuts at: 0 159 815 1893
Size: 159 656 1078
Fragments arranged by size:
1078 656 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 225 496 553 607 868 892 1772
Size: 202 23 271 57 54 261 24 880
Cuts at: 1772 1867 1893
Size: 95 26
Fragments arranged by size:
880 271 261 202 95 57 54 26
24 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1082 1375 1882 1893
Size: 1082 293 507 11
Fragments arranged by size:
1082 507 293 11
EcoO109I rG'GnC_Cy
Cuts at: 0 133 134 315 438 1157 1324 1461 1893
Size: 133 1 181 123 719 167 137 432
Fragments arranged by size:
719 432 181 167 137 133 123 1
FseI GG_CCGG'CC
Cuts at: 0 609 1893
Size: 609 1284
HaeII r_GCGC'y
Cuts at: 0 151 653 1893
Size: 151 502 1240
Fragments arranged by size:
1240 502 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 1247 1279 1341 1373 1893
Size: 187 32 1028 32 62 32 520
Fragments arranged by size:
1028 520 187 62 32 32 32
KasI G'GCGC_C
Cuts at: 0 147 649 1893
Size: 147 502 1244
Fragments arranged by size:
1244 502 147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 1893
Size: 98 1795
MscI TGG'CCA
Cuts at: 0 218 490 1243 1634 1893
Size: 218 272 753 391 259
Fragments arranged by size:
753 391 272 259 218
MslI CAynn'nnrTG
Cuts at: 0 676 805 1893
Size: 676 129 1088
Fragments arranged by size:
1088 676 129
MspA1I CmG'CkG
Cuts at: 0 83 357 363 408 470 1208 1362 1717
Size: 83 274 6 45 62 738 154 355
Cuts at: 1717 1780 1893
Size: 63 113
Fragments arranged by size:
738 355 274 154 113 83 63 62
45 6
NaeI GCC'GGC
Cuts at: 0 607 1893
Size: 607 1286
NarI GG'CG_CC
Cuts at: 0 148 650 1893
Size: 148 502 1243
Fragments arranged by size:
1243 502 148
NcoI C'CATG_G
Cuts at: 0 143 1521 1893
Size: 143 1378 372
Fragments arranged by size:
1378 372 143
NdeI CA'TA_TG
Cuts at: 0 673 1893
Size: 673 1220
NgoMIV G'CCGG_C
Cuts at: 0 605 1893
Size: 605 1288
NheI G'CTAG_C
Cuts at: 0 1278 1893
Size: 1278 615
NotI GC'GGCC_GC
Cuts at: 0 358 1893
Size: 358 1535
NspI r_CATG'y
Cuts at: 0 991 1893
Size: 991 902
PasI CC'CwG_GG
Cuts at: 0 1084 1893
Size: 1084 809
PflMI CCAn_nnn'nTGG
Cuts at: 0 144 936 1152 1527 1893
Size: 144 792 216 375 366
Fragments arranged by size:
792 375 366 216 144
PfoI T'CCnGG_A
Cuts at: 0 1883 1893
Size: 1883 10
PmlI CAC'GTG
Cuts at: 0 888 1893
Size: 888 1005
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1474 1506 1893
Size: 1474 32 387
Fragments arranged by size:
1474 387 32
PpuMI rG'GwC_Cy
Cuts at: 0 1461 1893
Size: 1461 432
PspOMI G'GGCC_C
Cuts at: 0 133 1324 1893
Size: 133 1191 569
Fragments arranged by size:
1191 569 133
PstI C_TGCA'G
Cuts at: 0 380 635 643 1586 1731 1893
Size: 380 255 8 943 145 162
Fragments arranged by size:
943 380 255 162 145 8
PvuII CAG'CTG
Cuts at: 0 1362 1717 1893
Size: 1362 355 176
Fragments arranged by size:
1362 355 176
SacI G_AGCT'C
Cuts at: 0 161 817 1893
Size: 161 656 1076
Fragments arranged by size:
1076 656 161
SbfI CC_TGCA'GG
Cuts at: 0 635 1893
Size: 635 1258
SfcI C'TryA_G
Cuts at: 0 376 631 639 1582 1727 1893
Size: 376 255 8 943 145 166
Fragments arranged by size:
943 376 255 166 145 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1774 1893
Size: 1774 119
SfoI GGC'GCC
Cuts at: 0 149 651 1893
Size: 149 502 1242
Fragments arranged by size:
1242 502 149
SmaI CCC'GGG
Cuts at: 0 154 260 1893
Size: 154 106 1633
Fragments arranged by size:
1633 154 106
StuI AGG'CCT
Cuts at: 0 128 1600 1893
Size: 128 1472 293
Fragments arranged by size:
1472 293 128
StyI C'CwwG_G
Cuts at: 0 4 143 1028 1104 1521 1559 1595 1893
Size: 4 139 885 76 417 38 36 298
Fragments arranged by size:
885 417 298 139 76 38 36 4
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1510 1687 1789 1883 1893
Size: 1510 177 102 94 10
Fragments arranged by size:
1510 177 102 94 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 239 271 1745 1777 1893
Size: 239 32 1474 32 116
Fragments arranged by size:
1474 239 116 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1012 1117 1602 1893
Size: 1012 105 485 291
Fragments arranged by size:
1012 485 291 105
XmaI C'CCGG_G
Cuts at: 0 152 258 1893
Size: 152 106 1635
Fragments arranged by size:
1635 152 106
XmnI GAAnn'nnTTC
Cuts at: 0 547 1893
Size: 547 1346
Enzymes that do cut:
AarI AcuI AflIII AloI AlwNI ApaI AscI AvaI
BanI BanII BbeI BbvCI BcgI BciVI BglI BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrFI BssHII BstAPI BstXI
BstYI Bsu36I BtgI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SbfI
SfcI SfiI SfoI SmaI StuI StyI TaqII TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaLI ApoI AseI AsiSI AvrII BaeI
BamHI BbsI BclI BfrBI BglII BpuEI BsaI BsaBI
BsiWI BsmI BspHI BsrBI BsrDI BsrGI BssSI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EcoRI
EcoRV FalI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NruI NsiI PacI PciI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI
ZraI