(Linear) (Six-base) MAPSORT of: KIEE1126.seq Check: 1730 from: 1 to: 1893 KIEE1126.orf With 182 enzymes: SgfI * July 15, 2010 18:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 632 1893 Size: 632 1261 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 225 496 553 868 1893 Size: 225 271 57 315 1025 Fragments arranged by size: 1025 315 271 225 57 AflIII A'CryG_T Cuts at: 0 385 1893 Size: 385 1508 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1474 1506 1893 Size: 1474 32 387 Fragments arranged by size: 1474 387 32 AlwNI CAG_nnn'CTG Cuts at: 0 846 936 1041 1717 1735 1893 Size: 846 90 105 676 18 158 Fragments arranged by size: 846 676 158 105 90 18 ApaI G_GGCC'C Cuts at: 0 137 1328 1893 Size: 137 1191 565 Fragments arranged by size: 1191 565 137 AscI GG'CGCG_CC Cuts at: 0 1233 1893 Size: 1233 660 AvaI C'yCGr_G Cuts at: 0 152 258 1893 Size: 152 106 1635 Fragments arranged by size: 1635 152 106 BanI G'GyrC_C Cuts at: 0 147 649 921 1893 Size: 147 502 272 972 Fragments arranged by size: 972 502 272 147 BanII G_rGCy'C Cuts at: 0 137 161 760 817 1062 1328 1673 1877 Size: 137 24 599 57 245 266 345 204 Cuts at: 1877 1893 Size: 16 Fragments arranged by size: 599 345 266 245 204 137 57 24 16 BbeI G_GCGC'C Cuts at: 0 151 653 1893 Size: 151 502 1240 Fragments arranged by size: 1240 502 151 BbvCI CC'TCA_GC Cuts at: 0 829 1893 Size: 829 1064 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1046 1080 1893 Size: 1046 34 813 Fragments arranged by size: 1046 813 34 BciVI GTATCCnnnnn_n' Cuts at: 0 846 1893 Size: 846 1047 BglI GCCn_nnn'nGGC Cuts at: 0 357 1513 1765 1774 1893 Size: 357 1156 252 9 119 Fragments arranged by size: 1156 357 252 119 9 BlpI GC'TnA_GC Cuts at: 0 766 811 1358 1577 1893 Size: 766 45 547 219 316 Fragments arranged by size: 766 547 316 219 45 Bme1580I G_kGCm'C Cuts at: 0 137 1328 1893 Size: 137 1191 565 Fragments arranged by size: 1191 565 137 BmgBI CAC'GTC Cuts at: 0 386 1893 Size: 386 1507 BmrI ACTGGGnnnn_n' Cuts at: 0 496 1893 Size: 496 1397 BmtI G_CTAG'C Cuts at: 0 1282 1893 Size: 1282 611 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 552 1893 Size: 552 1341 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 607 892 1772 1867 1893 Size: 202 405 285 880 95 26 Fragments arranged by size: 880 405 285 202 95 26 Bpu10I CC'TnA_GC Cuts at: 0 761 829 1283 1893 Size: 761 68 454 610 Fragments arranged by size: 761 610 454 68 BsaAI yAC'GTr Cuts at: 0 888 1893 Size: 888 1005 BsaHI Gr'CG_yC Cuts at: 0 148 650 1257 1893 Size: 148 502 607 636 Fragments arranged by size: 636 607 502 148 BsaWI w'CCGG_w Cuts at: 0 320 1341 1893 Size: 320 1021 552 Fragments arranged by size: 1021 552 320 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 239 269 1474 1504 1745 1775 1893 Size: 239 30 1205 30 241 30 118 Fragments arranged by size: 1205 241 239 118 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 502 544 629 632 1420 1477 1855 1893 Size: 502 42 85 3 788 57 378 38 Fragments arranged by size: 788 502 378 85 57 42 38 3 BseYI C'CCAG_C Cuts at: 0 137 699 1190 1735 1804 1893 Size: 137 562 491 545 69 89 Fragments arranged by size: 562 545 491 137 89 69 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 356 1054 1348 1703 1893 Size: 356 698 294 355 190 Fragments arranged by size: 698 356 355 294 190 BsiEI CG_ry'CG Cuts at: 0 361 606 1893 Size: 361 245 1287 Fragments arranged by size: 1287 361 245 BsiHKAI G_wGCw'C Cuts at: 0 161 798 817 1893 Size: 161 637 19 1076 Fragments arranged by size: 1076 637 161 19 BsmBI CGTCTCn'nnnn_ Cuts at: 0 259 376 1170 1893 Size: 259 117 794 723 Fragments arranged by size: 794 723 259 117 Bsp1286I G_dGCh'C Cuts at: 0 137 161 760 798 817 1062 1328 1673 Size: 137 24 599 38 19 245 266 345 Cuts at: 1673 1877 1893 Size: 204 16 Fragments arranged by size: 599 345 266 245 204 137 38 24 19 16 BspEI T'CCGG_A Cuts at: 0 320 1341 1893 Size: 320 1021 552 Fragments arranged by size: 1021 552 320 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 175 632 1893 Size: 175 457 1261 Fragments arranged by size: 1261 457 175 BsrFI r'CCGG_y Cuts at: 0 605 1893 Size: 605 1288 BssHII G'CGCG_C Cuts at: 0 1233 1893 Size: 1233 660 BstAPI GCAn_nnn'nTGC Cuts at: 0 236 1041 1717 1726 1893 Size: 236 805 676 9 167 Fragments arranged by size: 805 676 236 167 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 180 207 1893 Size: 180 27 1686 Fragments arranged by size: 1686 180 27 BstYI r'GATC_y Cuts at: 0 824 1893 Size: 824 1069 Bsu36I CC'TnA_GG Cuts at: 0 448 1372 1893 Size: 448 924 521 Fragments arranged by size: 924 521 448 BtgI C'CryG_G Cuts at: 0 143 1238 1521 1589 1760 1893 Size: 143 1095 283 68 171 133 Fragments arranged by size: 1095 283 171 143 133 68 BtsI GCAGTG_nn' Cuts at: 0 373 1893 Size: 373 1520 DrdI GACnn_nn'nnGTC Cuts at: 0 502 1893 Size: 502 1391 EaeI y'GGCC_r Cuts at: 0 216 358 488 603 607 890 1241 1592 Size: 216 142 130 115 4 283 351 351 Cuts at: 1592 1632 1893 Size: 40 261 Fragments arranged by size: 351 351 283 261 216 142 130 115 40 4 EagI C'GGCC_G Cuts at: 0 358 603 1893 Size: 358 245 1290 Fragments arranged by size: 1290 358 245 EarI CTCTTCn'nnn_ Cuts at: 0 304 1883 1893 Size: 304 1579 10 Fragments arranged by size: 1579 304 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 415 1110 1132 1893 Size: 415 695 22 761 Fragments arranged by size: 761 695 415 22 EcoICRI GAG'CTC Cuts at: 0 159 815 1893 Size: 159 656 1078 Fragments arranged by size: 1078 656 159 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 225 496 553 607 868 892 1772 Size: 202 23 271 57 54 261 24 880 Cuts at: 1772 1867 1893 Size: 95 26 Fragments arranged by size: 880 271 261 202 95 57 54 26 24 23 EcoNI CCTnn'n_nnAGG Cuts at: 0 1082 1375 1882 1893 Size: 1082 293 507 11 Fragments arranged by size: 1082 507 293 11 EcoO109I rG'GnC_Cy Cuts at: 0 133 134 315 438 1157 1324 1461 1893 Size: 133 1 181 123 719 167 137 432 Fragments arranged by size: 719 432 181 167 137 133 123 1 FseI GG_CCGG'CC Cuts at: 0 609 1893 Size: 609 1284 HaeII r_GCGC'y Cuts at: 0 151 653 1893 Size: 151 502 1240 Fragments arranged by size: 1240 502 151 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 187 219 1247 1279 1341 1373 1893 Size: 187 32 1028 32 62 32 520 Fragments arranged by size: 1028 520 187 62 32 32 32 KasI G'GCGC_C Cuts at: 0 147 649 1893 Size: 147 502 1244 Fragments arranged by size: 1244 502 147 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 98 1893 Size: 98 1795 MscI TGG'CCA Cuts at: 0 218 490 1243 1634 1893 Size: 218 272 753 391 259 Fragments arranged by size: 753 391 272 259 218 MslI CAynn'nnrTG Cuts at: 0 676 805 1893 Size: 676 129 1088 Fragments arranged by size: 1088 676 129 MspA1I CmG'CkG Cuts at: 0 83 357 363 408 470 1208 1362 1717 Size: 83 274 6 45 62 738 154 355 Cuts at: 1717 1780 1893 Size: 63 113 Fragments arranged by size: 738 355 274 154 113 83 63 62 45 6 NaeI GCC'GGC Cuts at: 0 607 1893 Size: 607 1286 NarI GG'CG_CC Cuts at: 0 148 650 1893 Size: 148 502 1243 Fragments arranged by size: 1243 502 148 NcoI C'CATG_G Cuts at: 0 143 1521 1893 Size: 143 1378 372 Fragments arranged by size: 1378 372 143 NdeI CA'TA_TG Cuts at: 0 673 1893 Size: 673 1220 NgoMIV G'CCGG_C Cuts at: 0 605 1893 Size: 605 1288 NheI G'CTAG_C Cuts at: 0 1278 1893 Size: 1278 615 NotI GC'GGCC_GC Cuts at: 0 358 1893 Size: 358 1535 NspI r_CATG'y Cuts at: 0 991 1893 Size: 991 902 PasI CC'CwG_GG Cuts at: 0 1084 1893 Size: 1084 809 PflMI CCAn_nnn'nTGG Cuts at: 0 144 936 1152 1527 1893 Size: 144 792 216 375 366 Fragments arranged by size: 792 375 366 216 144 PfoI T'CCnGG_A Cuts at: 0 1883 1893 Size: 1883 10 PmlI CAC'GTG Cuts at: 0 888 1893 Size: 888 1005 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1474 1506 1893 Size: 1474 32 387 Fragments arranged by size: 1474 387 32 PpuMI rG'GwC_Cy Cuts at: 0 1461 1893 Size: 1461 432 PspOMI G'GGCC_C Cuts at: 0 133 1324 1893 Size: 133 1191 569 Fragments arranged by size: 1191 569 133 PstI C_TGCA'G Cuts at: 0 380 635 643 1586 1731 1893 Size: 380 255 8 943 145 162 Fragments arranged by size: 943 380 255 162 145 8 PvuII CAG'CTG Cuts at: 0 1362 1717 1893 Size: 1362 355 176 Fragments arranged by size: 1362 355 176 SacI G_AGCT'C Cuts at: 0 161 817 1893 Size: 161 656 1076 Fragments arranged by size: 1076 656 161 SbfI CC_TGCA'GG Cuts at: 0 635 1893 Size: 635 1258 SfcI C'TryA_G Cuts at: 0 376 631 639 1582 1727 1893 Size: 376 255 8 943 145 166 Fragments arranged by size: 943 376 255 166 145 8 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1774 1893 Size: 1774 119 SfoI GGC'GCC Cuts at: 0 149 651 1893 Size: 149 502 1242 Fragments arranged by size: 1242 502 149 SmaI CCC'GGG Cuts at: 0 154 260 1893 Size: 154 106 1633 Fragments arranged by size: 1633 154 106 StuI AGG'CCT Cuts at: 0 128 1600 1893 Size: 128 1472 293 Fragments arranged by size: 1472 293 128 StyI C'CwwG_G Cuts at: 0 4 143 1028 1104 1521 1559 1595 1893 Size: 4 139 885 76 417 38 36 298 Fragments arranged by size: 885 417 298 139 76 38 36 4 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1510 1687 1789 1883 1893 Size: 1510 177 102 94 10 Fragments arranged by size: 1510 177 102 94 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 239 271 1745 1777 1893 Size: 239 32 1474 32 116 Fragments arranged by size: 1474 239 116 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1012 1117 1602 1893 Size: 1012 105 485 291 Fragments arranged by size: 1012 485 291 105 XmaI C'CCGG_G Cuts at: 0 152 258 1893 Size: 152 106 1635 Fragments arranged by size: 1635 152 106 XmnI GAAnn'nnTTC Cuts at: 0 547 1893 Size: 547 1346 Enzymes that do cut: AarI AcuI AflIII AloI AlwNI ApaI AscI AvaI BanI BanII BbeI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SbfI SfcI SfiI SfoI SmaI StuI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BclI BfrBI BglII BpuEI BsaI BsaBI BsiWI BsmI BspHI BsrBI BsrDI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI ZraI