Restriction map for
pFN21AE1123
(Linear) (Six-base) MAPSORT of: KIEE1123.seq Check: 9203 from: 1 to: 1368
KIEE1123.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 602 1368
Size: 602 766
AccI GT'mk_AC
Cuts at: 0 1096 1368
Size: 1096 272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1368
Size: 671 697
AlwNI CAG_nnn'CTG
Cuts at: 0 258 1227 1368
Size: 258 969 141
Fragments arranged by size:
969 258 141
ApaLI G'TGCA_C
Cuts at: 0 682 1368
Size: 682 686
ApoI r'AATT_y
Cuts at: 0 634 1368
Size: 634 734
AseI AT'TA_AT
Cuts at: 0 163 836 1368
Size: 163 673 532
Fragments arranged by size:
673 532 163
AvaI C'yCGr_G
Cuts at: 0 885 1368
Size: 885 483
BanI G'GyrC_C
Cuts at: 0 1192 1368
Size: 1192 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 37 328 808 953 1368
Size: 37 291 480 145 415
Fragments arranged by size:
480 415 291 145 37
BclI T'GATC_A
Cuts at: 0 470 1368
Size: 470 898
BglI GCCn_nnn'nGGC
Cuts at: 0 100 1368
Size: 100 1268
BlpI GC'TnA_GC
Cuts at: 0 875 1368
Size: 875 493
Bme1580I G_kGCm'C
Cuts at: 0 686 1137 1368
Size: 686 451 231
Fragments arranged by size:
686 451 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 976 1368
Size: 581 395 392
Fragments arranged by size:
581 395 392
Bpu10I CC'TnA_GC
Cuts at: 0 251 1368
Size: 251 1117
BsaI GGTCTCn'nnnn_
Cuts at: 0 370 1368
Size: 370 998
BsaAI yAC'GTr
Cuts at: 0 60 1368
Size: 60 1308
BsaBI GATnn'nnATC
Cuts at: 0 1078 1368
Size: 1078 290
BsaHI Gr'CG_yC
Cuts at: 0 149 1368
Size: 149 1219
BsaWI w'CCGG_w
Cuts at: 0 1082 1368
Size: 1082 286
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 1177 1368
Size: 25 1152 191
Fragments arranged by size:
1152 191 25
BseYI C'CCAG_C
Cuts at: 0 69 258 520 1368
Size: 69 189 262 848
Fragments arranged by size:
848 262 189 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1240 1368
Size: 928 312 128
Fragments arranged by size:
928 312 128
BsiHKAI G_wGCw'C
Cuts at: 0 25 686 1368
Size: 25 661 682
Fragments arranged by size:
682 661 25
Bsp1286I G_dGCh'C
Cuts at: 0 25 686 1137 1368
Size: 25 661 451 231
Fragments arranged by size:
661 451 231 25
BspEI T'CCGG_A
Cuts at: 0 1082 1368
Size: 1082 286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 602 950 1368
Size: 602 348 418
Fragments arranged by size:
602 418 348
BsrBI CCG'CTC
Cuts at: 0 86 1368
Size: 86 1282
BsrDI GCAATG_nn'
Cuts at: 0 869 1368
Size: 869 499
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1338 1368
Size: 1338 30
BstZ17I GTA'TAC
Cuts at: 0 1097 1368
Size: 1097 271
Bsu36I CC'TnA_GG
Cuts at: 0 108 1368
Size: 108 1260
BtgI C'CryG_G
Cuts at: 0 437 1368
Size: 437 931
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 27 1368
Size: 27 1341
EaeI y'GGCC_r
Cuts at: 0 92 1235 1368
Size: 92 1143 133
Fragments arranged by size:
1143 133 92
EarI CTCTTCn'nnn_
Cuts at: 0 13 183 595 647 1286 1368
Size: 13 170 412 52 639 82
Fragments arranged by size:
639 412 170 82 52 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1317 1368
Size: 1317 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 671 976 1368
Size: 581 90 305 392
Fragments arranged by size:
581 392 305 90
EcoO109I rG'GnC_Cy
Cuts at: 0 208 270 1368
Size: 208 62 1098
Fragments arranged by size:
1098 208 62
EcoRI G'AATT_C
Cuts at: 0 634 1368
Size: 634 734
EcoRV GAT'ATC
Cuts at: 0 765 1368
Size: 765 603
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 291 1368
Size: 291 1077
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 417 449 749 754 781 786 1018 1050
Size: 417 32 300 5 27 5 232 32
Cuts at: 1050 1306 1338 1368
Size: 256 32 30
Fragments arranged by size:
417 300 256 232 32 32 32 30
27 5 5
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 1368
Size: 1016 352
MscI TGG'CCA
Cuts at: 0 94 1368
Size: 94 1274
MspA1I CmG'CkG
Cuts at: 0 1224 1368
Size: 1224 144
NcoI C'CATG_G
Cuts at: 0 437 1368
Size: 437 931
NspI r_CATG'y
Cuts at: 0 146 918 1288 1368
Size: 146 772 370 80
Fragments arranged by size:
772 370 146 80
PasI CC'CwG_GG
Cuts at: 0 750 1368
Size: 750 618
PflMI CCAn_nnn'nTGG
Cuts at: 0 918 1368
Size: 918 450
PfoI T'CCnGG_A
Cuts at: 0 213 1368
Size: 213 1155
PmlI CAC'GTG
Cuts at: 0 60 1368
Size: 60 1308
PpuMI rG'GwC_Cy
Cuts at: 0 208 270 1368
Size: 208 62 1098
Fragments arranged by size:
1098 208 62
PvuII CAG'CTG
Cuts at: 0 1224 1368
Size: 1224 144
SapI GCTCTTCn'nnn_
Cuts at: 0 13 183 1368
Size: 13 170 1185
Fragments arranged by size:
1185 170 13
SmaI CCC'GGG
Cuts at: 0 887 1368
Size: 887 481
StyI C'CwwG_G
Cuts at: 0 380 437 1368
Size: 380 57 931
Fragments arranged by size:
931 380 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1082 1368
Size: 1082 286
TatI w'GTAC_w
Cuts at: 0 854 1358 1368
Size: 854 504 10
Fragments arranged by size:
854 504 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 913 945 1368
Size: 913 32 423
Fragments arranged by size:
913 423 32
XmaI C'CCGG_G
Cuts at: 0 885 1368
Size: 885 483
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaLI ApoI AseI AvaI
BanI BbsI BclI BglI BlpI Bme1580I BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BstXI BstZ17I
Bsu36I BtgI BtgZI EaeI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI Hin4I MmeI MscI MspA1I NcoI
NspI PasI PflMI PfoI PmlI PpuMI PvuII SapI
SmaI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AsiSI AvrII BaeI
BamHI BanII BbeI BbvCI BcgI BciVI BfrBI BglII
BmgBI BmrI BmtI BplI BpuEI BsaXI BsiEI BsiWI
BsmI BsmBI BspHI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtsI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmnI ZraI