(Linear) (Six-base) MAPSORT of: KIEE1122.seq Check: 8346 from: 1 to: 1299 KIEE1122.orf With 182 enzymes: SgfI * July 15, 2010 18:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 611 1299 Size: 611 688 AccI GT'mk_AC Cuts at: 0 1282 1299 Size: 1282 17 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 719 899 1299 Size: 241 478 180 400 Fragments arranged by size: 478 400 241 180 AleI CACnn'nnGTG Cuts at: 0 1219 1299 Size: 1219 80 AlwNI CAG_nnn'CTG Cuts at: 0 269 1299 Size: 269 1030 ApaI G_GGCC'C Cuts at: 0 616 973 1299 Size: 616 357 326 Fragments arranged by size: 616 357 326 ApaLI G'TGCA_C Cuts at: 0 887 1299 Size: 887 412 AvaI C'yCGr_G Cuts at: 0 803 1072 1299 Size: 803 269 227 Fragments arranged by size: 803 269 227 BanI G'GyrC_C Cuts at: 0 164 852 921 1065 1269 1299 Size: 164 688 69 144 204 30 Fragments arranged by size: 688 204 164 144 69 30 BanII G_rGCy'C Cuts at: 0 154 616 973 996 1146 1299 Size: 154 462 357 23 150 153 Fragments arranged by size: 462 357 154 153 150 23 BbsI GAAGACnn'nnnn_ Cuts at: 0 43 954 1299 Size: 43 911 345 Fragments arranged by size: 911 345 43 BbvCI CC'TCA_GC Cuts at: 0 1104 1299 Size: 1104 195 BclI T'GATC_A Cuts at: 0 1152 1299 Size: 1152 147 BglI GCCn_nnn'nGGC Cuts at: 0 208 1299 Size: 208 1091 BlpI GC'TnA_GC Cuts at: 0 148 358 1299 Size: 148 210 941 Fragments arranged by size: 941 210 148 Bme1580I G_kGCm'C Cuts at: 0 167 616 826 855 891 973 1068 1299 Size: 167 449 210 29 36 82 95 231 Fragments arranged by size: 449 231 210 167 95 82 36 29 BmrI ACTGGGnnnn_n' Cuts at: 0 123 1299 Size: 123 1176 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 288 291 424 620 632 1299 Size: 288 3 133 196 12 667 Fragments arranged by size: 667 288 196 133 12 3 Bpu10I CC'TnA_GC Cuts at: 0 1104 1299 Size: 1104 195 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1117 1299 Size: 1117 182 BsaI GGTCTCn'nnnn_ Cuts at: 0 891 1002 1299 Size: 891 111 297 Fragments arranged by size: 891 297 111 BsaHI Gr'CG_yC Cuts at: 0 286 861 1299 Size: 286 575 438 Fragments arranged by size: 575 438 286 BsaWI w'CCGG_w Cuts at: 0 230 1299 Size: 230 1069 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 412 442 1299 Size: 412 30 857 Fragments arranged by size: 857 412 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 391 1076 1299 Size: 391 685 223 Fragments arranged by size: 685 391 223 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 664 1299 Size: 139 525 635 Fragments arranged by size: 635 525 139 BsiHKAI G_wGCw'C Cuts at: 0 563 593 891 1299 Size: 563 30 298 408 Fragments arranged by size: 563 408 298 30 BsmBI CGTCTCn'nnnn_ Cuts at: 0 75 1299 Size: 75 1224 Bsp1286I G_dGCh'C Cuts at: 0 154 167 563 593 616 826 855 891 Size: 154 13 396 30 23 210 29 36 Cuts at: 891 973 996 1068 1146 1299 Size: 82 23 72 78 153 Fragments arranged by size: 396 210 154 153 82 78 72 36 30 29 23 23 13 BspEI T'CCGG_A Cuts at: 0 230 1299 Size: 230 1069 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 482 587 611 1299 Size: 482 105 24 688 Fragments arranged by size: 688 482 105 24 BsrBI CCG'CTC Cuts at: 0 314 1299 Size: 314 985 BsrFI r'CCGG_y Cuts at: 0 143 692 1299 Size: 143 549 607 Fragments arranged by size: 607 549 143 BstAPI GCAn_nnn'nTGC Cuts at: 0 171 666 675 1299 Size: 171 495 9 624 Fragments arranged by size: 624 495 171 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 417 776 1019 1299 Size: 417 359 243 280 Fragments arranged by size: 417 359 280 243 BstZ17I GTA'TAC Cuts at: 0 1283 1299 Size: 1283 16 Bsu36I CC'TnA_GG Cuts at: 0 620 1088 1299 Size: 620 468 211 Fragments arranged by size: 620 468 211 BtgI C'CryG_G Cuts at: 0 501 1299 Size: 501 798 BtsI GCAGTG_nn' Cuts at: 0 1142 1157 1299 Size: 1142 15 142 Fragments arranged by size: 1142 142 15 DraIII CAC_nnn'GTG Cuts at: 0 171 1299 Size: 171 1128 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 226 1299 Size: 226 1073 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 288 291 424 620 632 719 899 Size: 241 47 3 133 196 12 87 180 Cuts at: 899 1299 Size: 400 Fragments arranged by size: 400 241 196 180 133 87 47 12 3 EcoO109I rG'GnC_Cy Cuts at: 0 237 624 933 969 970 1299 Size: 237 387 309 36 1 329 Fragments arranged by size: 387 329 309 237 36 1 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1068 1100 1299 Size: 1068 32 199 Fragments arranged by size: 1068 199 32 HincII GTy'rAC Cuts at: 0 791 1299 Size: 791 508 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 398 1299 Size: 398 901 MslI CAynn'nnrTG Cuts at: 0 1219 1299 Size: 1219 80 MspA1I CmG'CkG Cuts at: 0 30 357 503 877 1241 1299 Size: 30 327 146 374 364 58 Fragments arranged by size: 374 364 327 146 58 30 NaeI GCC'GGC Cuts at: 0 145 694 1299 Size: 145 549 605 Fragments arranged by size: 605 549 145 NgoMIV G'CCGG_C Cuts at: 0 143 692 1299 Size: 143 549 607 Fragments arranged by size: 607 549 143 PasI CC'CwG_GG Cuts at: 0 609 1299 Size: 609 690 PflMI CCAn_nnn'nTGG Cuts at: 0 204 1299 Size: 204 1095 PfoI T'CCnGG_A Cuts at: 0 440 1003 1299 Size: 440 563 296 Fragments arranged by size: 563 440 296 PpuMI rG'GwC_Cy Cuts at: 0 237 1299 Size: 237 1062 PspOMI G'GGCC_C Cuts at: 0 612 969 1299 Size: 612 357 330 Fragments arranged by size: 612 357 330 PstI C_TGCA'G Cuts at: 0 35 548 659 876 1299 Size: 35 513 111 217 423 Fragments arranged by size: 513 423 217 111 35 PvuII CAG'CTG Cuts at: 0 30 877 1299 Size: 30 847 422 Fragments arranged by size: 847 422 30 SacII CC_GC'GG Cuts at: 0 504 1299 Size: 504 795 SexAI A'CCwGG_T Cuts at: 0 167 1299 Size: 167 1132 SfcI C'TryA_G Cuts at: 0 31 544 655 872 1299 Size: 31 513 111 217 427 Fragments arranged by size: 513 427 217 111 31 SmaI CCC'GGG Cuts at: 0 805 1299 Size: 805 494 SmlI C'TyrA_G Cuts at: 0 1096 1299 Size: 1096 203 StuI AGG'CCT Cuts at: 0 455 799 1102 1299 Size: 455 344 303 197 Fragments arranged by size: 455 344 303 197 StyI C'CwwG_G Cuts at: 0 318 722 1299 Size: 318 404 577 Fragments arranged by size: 577 404 318 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 124 1125 1299 Size: 124 1001 174 Fragments arranged by size: 1001 174 124 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 19 776 915 1299 Size: 19 757 139 384 Fragments arranged by size: 757 384 139 19 XmaI C'CCGG_G Cuts at: 0 803 1299 Size: 803 496 XmnI GAAnn'nnTTC Cuts at: 0 226 1299 Size: 226 1073 Enzymes that do cut: AarI AccI AcuI AleI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BclI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI BstXI BstZ17I Bsu36I BtgI BtsI DraIII EciI Eco57MI EcoO109I Hin4I HincII MmeI MslI MspA1I NaeI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacII SexAI SfcI SmaI SmlI StuI StyI TaqII XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglII BmgBI BmtI BplI BsaAI BsaBI BseYI BsiEI BsiWI BsmI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI BtgZI ClaI CspCI DraI DrdI EaeI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI ZraI