Restriction map for
pFN21AE1122
(Linear) (Six-base) MAPSORT of: KIEE1122.seq Check: 8346 from: 1 to: 1299
KIEE1122.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 611 1299
Size: 611 688
AccI GT'mk_AC
Cuts at: 0 1282 1299
Size: 1282 17
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 719 899 1299
Size: 241 478 180 400
Fragments arranged by size:
478 400 241 180
AleI CACnn'nnGTG
Cuts at: 0 1219 1299
Size: 1219 80
AlwNI CAG_nnn'CTG
Cuts at: 0 269 1299
Size: 269 1030
ApaI G_GGCC'C
Cuts at: 0 616 973 1299
Size: 616 357 326
Fragments arranged by size:
616 357 326
ApaLI G'TGCA_C
Cuts at: 0 887 1299
Size: 887 412
AvaI C'yCGr_G
Cuts at: 0 803 1072 1299
Size: 803 269 227
Fragments arranged by size:
803 269 227
BanI G'GyrC_C
Cuts at: 0 164 852 921 1065 1269 1299
Size: 164 688 69 144 204 30
Fragments arranged by size:
688 204 164 144 69 30
BanII G_rGCy'C
Cuts at: 0 154 616 973 996 1146 1299
Size: 154 462 357 23 150 153
Fragments arranged by size:
462 357 154 153 150 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 43 954 1299
Size: 43 911 345
Fragments arranged by size:
911 345 43
BbvCI CC'TCA_GC
Cuts at: 0 1104 1299
Size: 1104 195
BclI T'GATC_A
Cuts at: 0 1152 1299
Size: 1152 147
BglI GCCn_nnn'nGGC
Cuts at: 0 208 1299
Size: 208 1091
BlpI GC'TnA_GC
Cuts at: 0 148 358 1299
Size: 148 210 941
Fragments arranged by size:
941 210 148
Bme1580I G_kGCm'C
Cuts at: 0 167 616 826 855 891 973 1068 1299
Size: 167 449 210 29 36 82 95 231
Fragments arranged by size:
449 231 210 167 95 82 36 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 123 1299
Size: 123 1176
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 291 424 620 632 1299
Size: 288 3 133 196 12 667
Fragments arranged by size:
667 288 196 133 12 3
Bpu10I CC'TnA_GC
Cuts at: 0 1104 1299
Size: 1104 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1117 1299
Size: 1117 182
BsaI GGTCTCn'nnnn_
Cuts at: 0 891 1002 1299
Size: 891 111 297
Fragments arranged by size:
891 297 111
BsaHI Gr'CG_yC
Cuts at: 0 286 861 1299
Size: 286 575 438
Fragments arranged by size:
575 438 286
BsaWI w'CCGG_w
Cuts at: 0 230 1299
Size: 230 1069
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 412 442 1299
Size: 412 30 857
Fragments arranged by size:
857 412 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 1076 1299
Size: 391 685 223
Fragments arranged by size:
685 391 223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 664 1299
Size: 139 525 635
Fragments arranged by size:
635 525 139
BsiHKAI G_wGCw'C
Cuts at: 0 563 593 891 1299
Size: 563 30 298 408
Fragments arranged by size:
563 408 298 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 75 1299
Size: 75 1224
Bsp1286I G_dGCh'C
Cuts at: 0 154 167 563 593 616 826 855 891
Size: 154 13 396 30 23 210 29 36
Cuts at: 891 973 996 1068 1146 1299
Size: 82 23 72 78 153
Fragments arranged by size:
396 210 154 153 82 78 72 36
30 29 23 23 13
BspEI T'CCGG_A
Cuts at: 0 230 1299
Size: 230 1069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 482 587 611 1299
Size: 482 105 24 688
Fragments arranged by size:
688 482 105 24
BsrBI CCG'CTC
Cuts at: 0 314 1299
Size: 314 985
BsrFI r'CCGG_y
Cuts at: 0 143 692 1299
Size: 143 549 607
Fragments arranged by size:
607 549 143
BstAPI GCAn_nnn'nTGC
Cuts at: 0 171 666 675 1299
Size: 171 495 9 624
Fragments arranged by size:
624 495 171 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 776 1019 1299
Size: 417 359 243 280
Fragments arranged by size:
417 359 280 243
BstZ17I GTA'TAC
Cuts at: 0 1283 1299
Size: 1283 16
Bsu36I CC'TnA_GG
Cuts at: 0 620 1088 1299
Size: 620 468 211
Fragments arranged by size:
620 468 211
BtgI C'CryG_G
Cuts at: 0 501 1299
Size: 501 798
BtsI GCAGTG_nn'
Cuts at: 0 1142 1157 1299
Size: 1142 15 142
Fragments arranged by size:
1142 142 15
DraIII CAC_nnn'GTG
Cuts at: 0 171 1299
Size: 171 1128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 226 1299
Size: 226 1073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 288 291 424 620 632 719 899
Size: 241 47 3 133 196 12 87 180
Cuts at: 899 1299
Size: 400
Fragments arranged by size:
400 241 196 180 133 87 47 12
3
EcoO109I rG'GnC_Cy
Cuts at: 0 237 624 933 969 970 1299
Size: 237 387 309 36 1 329
Fragments arranged by size:
387 329 309 237 36 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1068 1100 1299
Size: 1068 32 199
Fragments arranged by size:
1068 199 32
HincII GTy'rAC
Cuts at: 0 791 1299
Size: 791 508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 1299
Size: 398 901
MslI CAynn'nnrTG
Cuts at: 0 1219 1299
Size: 1219 80
MspA1I CmG'CkG
Cuts at: 0 30 357 503 877 1241 1299
Size: 30 327 146 374 364 58
Fragments arranged by size:
374 364 327 146 58 30
NaeI GCC'GGC
Cuts at: 0 145 694 1299
Size: 145 549 605
Fragments arranged by size:
605 549 145
NgoMIV G'CCGG_C
Cuts at: 0 143 692 1299
Size: 143 549 607
Fragments arranged by size:
607 549 143
PasI CC'CwG_GG
Cuts at: 0 609 1299
Size: 609 690
PflMI CCAn_nnn'nTGG
Cuts at: 0 204 1299
Size: 204 1095
PfoI T'CCnGG_A
Cuts at: 0 440 1003 1299
Size: 440 563 296
Fragments arranged by size:
563 440 296
PpuMI rG'GwC_Cy
Cuts at: 0 237 1299
Size: 237 1062
PspOMI G'GGCC_C
Cuts at: 0 612 969 1299
Size: 612 357 330
Fragments arranged by size:
612 357 330
PstI C_TGCA'G
Cuts at: 0 35 548 659 876 1299
Size: 35 513 111 217 423
Fragments arranged by size:
513 423 217 111 35
PvuII CAG'CTG
Cuts at: 0 30 877 1299
Size: 30 847 422
Fragments arranged by size:
847 422 30
SacII CC_GC'GG
Cuts at: 0 504 1299
Size: 504 795
SexAI A'CCwGG_T
Cuts at: 0 167 1299
Size: 167 1132
SfcI C'TryA_G
Cuts at: 0 31 544 655 872 1299
Size: 31 513 111 217 427
Fragments arranged by size:
513 427 217 111 31
SmaI CCC'GGG
Cuts at: 0 805 1299
Size: 805 494
SmlI C'TyrA_G
Cuts at: 0 1096 1299
Size: 1096 203
StuI AGG'CCT
Cuts at: 0 455 799 1102 1299
Size: 455 344 303 197
Fragments arranged by size:
455 344 303 197
StyI C'CwwG_G
Cuts at: 0 318 722 1299
Size: 318 404 577
Fragments arranged by size:
577 404 318
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 124 1125 1299
Size: 124 1001 174
Fragments arranged by size:
1001 174 124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 19 776 915 1299
Size: 19 757 139 384
Fragments arranged by size:
757 384 139 19
XmaI C'CCGG_G
Cuts at: 0 803 1299
Size: 803 496
XmnI GAAnn'nnTTC
Cuts at: 0 226 1299
Size: 226 1073
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BbvCI BclI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BstAPI BstXI BstZ17I Bsu36I BtgI BtsI DraIII
EciI Eco57MI EcoO109I Hin4I HincII MmeI MslI MspA1I
NaeI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SacII SexAI SfcI SmaI SmlI StuI StyI
TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BfrBI BglII BmgBI BmtI
BplI BsaAI BsaBI BseYI BsiEI BsiWI BsmI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI BtgZI
ClaI CspCI DraI DrdI EaeI EagI EarI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XhoI ZraI