Restriction map for
pFN21AE1121
(Linear) (Six-base) MAPSORT of: KIEE1121.seq Check: 8268 from: 1 to: 1098
KIEE1121.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 299 895 1098
Size: 299 596 203
Fragments arranged by size:
596 299 203
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 1098
Size: 779 319
AleI CACnn'nnGTG
Cuts at: 0 166 1098
Size: 166 932
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 109 307 1098
Size: 109 198 791
Fragments arranged by size:
791 198 109
AlwNI CAG_nnn'CTG
Cuts at: 0 9 81 318 567 588 1098
Size: 9 72 237 249 21 510
Fragments arranged by size:
510 249 237 72 21 9
ApaI G_GGCC'C
Cuts at: 0 177 429 628 1098
Size: 177 252 199 470
Fragments arranged by size:
470 252 199 177
AvaI C'yCGr_G
Cuts at: 0 52 422 428 855 1098
Size: 52 370 6 427 243
Fragments arranged by size:
427 370 243 52 6
BanI G'GyrC_C
Cuts at: 0 117 640 675 778 883 1098
Size: 117 523 35 103 105 215
Fragments arranged by size:
523 215 117 105 103 35
BanII G_rGCy'C
Cuts at: 0 177 429 628 698 741 854 1098
Size: 177 252 199 70 43 113 244
Fragments arranged by size:
252 244 199 177 113 70 43
BbeI G_GCGC'C
Cuts at: 0 644 679 1098
Size: 644 35 419
Fragments arranged by size:
644 419 35
BglI GCCn_nnn'nGGC
Cuts at: 0 12 684 1086 1098
Size: 12 672 402 12
Fragments arranged by size:
672 402 12 12
BlpI GC'TnA_GC
Cuts at: 0 568 1098
Size: 568 530
Bme1580I G_kGCm'C
Cuts at: 0 27 122 177 429 628 781 886 1098
Size: 27 95 55 252 199 153 105 212
Fragments arranged by size:
252 212 199 153 105 95 55 27
BmgBI CAC'GTC
Cuts at: 0 537 1098
Size: 537 561
BmrI ACTGGGnnnn_n'
Cuts at: 0 171 849 1098
Size: 171 678 249
Fragments arranged by size:
678 249 171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 981 1098
Size: 981 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 724 868 1075 1098
Size: 477 247 144 207 23
Fragments arranged by size:
477 247 207 144 23
Bpu10I CC'TnA_GC
Cuts at: 0 740 1098
Size: 740 358
BsaAI yAC'GTr
Cuts at: 0 168 183 1098
Size: 168 15 915
Fragments arranged by size:
915 168 15
BsaHI Gr'CG_yC
Cuts at: 0 634 641 676 1098
Size: 634 7 35 422
Fragments arranged by size:
634 422 35 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 451 463 481 493 1098
Size: 451 12 18 12 605
Fragments arranged by size:
605 451 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 1098
Size: 160 938
BseYI C'CCAG_C
Cuts at: 0 20 698 1098
Size: 20 678 400
Fragments arranged by size:
678 400 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 373 628 694 1063 1071 1098
Size: 361 12 255 66 369 8 27
Fragments arranged by size:
369 361 255 66 27 12 8
BsiHKAI G_wGCw'C
Cuts at: 0 741 1098
Size: 741 357
Bsp1286I G_dGCh'C
Cuts at: 0 27 122 177 429 628 698 741 781
Size: 27 95 55 252 199 70 43 40
Cuts at: 781 854 886 1098
Size: 73 32 212
Fragments arranged by size:
252 212 199 95 73 70 55 43
40 32 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 251 299 821 895 1098
Size: 251 48 522 74 203
Fragments arranged by size:
522 251 203 74 48
BsrBI CCG'CTC
Cuts at: 0 215 518 1098
Size: 215 303 580
Fragments arranged by size:
580 303 215
BsrFI r'CCGG_y
Cuts at: 0 256 369 572 890 1098
Size: 256 113 203 318 208
Fragments arranged by size:
318 256 208 203 113
BsrGI T'GTAC_A
Cuts at: 0 803 1098
Size: 803 295
BssSI C'ACGA_G
Cuts at: 0 511 1075 1098
Size: 511 564 23
Fragments arranged by size:
564 511 23
BstAPI GCAn_nnn'nTGC
Cuts at: 0 318 348 588 785 1098
Size: 318 30 240 197 313
Fragments arranged by size:
318 313 240 197 30
BstEII G'GTnAC_C
Cuts at: 0 432 1098
Size: 432 666
BtgI C'CryG_G
Cuts at: 0 204 219 1098
Size: 204 15 879
Fragments arranged by size:
879 204 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 933 1098
Size: 933 165
EaeI y'GGCC_r
Cuts at: 0 44 254 922 1098
Size: 44 210 668 176
Fragments arranged by size:
668 210 176 44
EarI CTCTTCn'nnn_
Cuts at: 0 364 1098
Size: 364 734
EcoICRI GAG'CTC
Cuts at: 0 739 1098
Size: 739 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 724 779 868 1075 1098
Size: 477 247 55 89 207 23
Fragments arranged by size:
477 247 207 89 55 23
EcoO109I rG'GnC_Cy
Cuts at: 0 173 906 934 1010 1098
Size: 173 733 28 76 88
Fragments arranged by size:
733 173 88 76 28
HaeII r_GCGC'y
Cuts at: 0 422 644 679 1098
Size: 422 222 35 419
Fragments arranged by size:
422 419 222 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 603 635 932 964 1098
Size: 451 32 120 32 297 32 134
Fragments arranged by size:
451 297 134 120 32 32 32
KasI G'GCGC_C
Cuts at: 0 640 675 1098
Size: 640 35 423
Fragments arranged by size:
640 423 35
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 494 1098
Size: 254 240 604
Fragments arranged by size:
604 254 240
MscI TGG'CCA
Cuts at: 0 46 1098
Size: 46 1052
MslI CAynn'nnrTG
Cuts at: 0 166 1039 1098
Size: 166 873 59
Fragments arranged by size:
873 166 59
MspA1I CmG'CkG
Cuts at: 0 102 221 315 567 588 630 702 785
Size: 102 119 94 252 21 42 72 83
Cuts at: 785 1055 1098
Size: 270 43
Fragments arranged by size:
270 252 119 102 94 83 72 43
42 21
NaeI GCC'GGC
Cuts at: 0 258 574 892 1098
Size: 258 316 318 206
Fragments arranged by size:
318 316 258 206
NarI GG'CG_CC
Cuts at: 0 641 676 1098
Size: 641 35 422
Fragments arranged by size:
641 422 35
NgoMIV G'CCGG_C
Cuts at: 0 256 572 890 1098
Size: 256 316 318 208
Fragments arranged by size:
318 316 256 208
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 651 1098
Size: 183 468 447
Fragments arranged by size:
468 447 183
PfoI T'CCnGG_A
Cuts at: 0 362 1098
Size: 362 736
PmlI CAC'GTG
Cuts at: 0 168 1098
Size: 168 930
PspOMI G'GGCC_C
Cuts at: 0 173 425 624 1098
Size: 173 252 199 474
Fragments arranged by size:
474 252 199 173
PstI C_TGCA'G
Cuts at: 0 107 197 818 1098
Size: 107 90 621 280
Fragments arranged by size:
621 280 107 90
PvuII CAG'CTG
Cuts at: 0 102 567 702 1055 1098
Size: 102 465 135 353 43
Fragments arranged by size:
465 353 135 102 43
SacI G_AGCT'C
Cuts at: 0 741 1098
Size: 741 357
SacII CC_GC'GG
Cuts at: 0 222 1098
Size: 222 876
SapI GCTCTTCn'nnn_
Cuts at: 0 364 1098
Size: 364 734
SbfI CC_TGCA'GG
Cuts at: 0 818 1098
Size: 818 280
SfcI C'TryA_G
Cuts at: 0 103 193 595 814 1098
Size: 103 90 402 219 284
Fragments arranged by size:
402 284 219 103 90
SfoI GGC'GCC
Cuts at: 0 642 677 1098
Size: 642 35 421
Fragments arranged by size:
642 421 35
SmaI CCC'GGG
Cuts at: 0 54 424 430 857 1098
Size: 54 370 6 427 241
Fragments arranged by size:
427 370 241 54 6
SrfI GCCC'GGGC
Cuts at: 0 424 1098
Size: 424 674
StuI AGG'CCT
Cuts at: 0 529 1098
Size: 529 569
StyI C'CwwG_G
Cuts at: 0 377 723 1081 1098
Size: 377 346 358 17
Fragments arranged by size:
377 358 346 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 178 556 787 831 1098
Size: 21 157 378 231 44 267
Fragments arranged by size:
378 267 231 157 44 21
TatI w'GTAC_w
Cuts at: 0 803 1098
Size: 803 295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 1014 1098
Size: 333 681 84
Fragments arranged by size:
681 333 84
XmaI C'CCGG_G
Cuts at: 0 52 422 428 855 1098
Size: 52 370 6 427 243
Fragments arranged by size:
427 370 243 52 6
XmnI GAAnn'nnTTC
Cuts at: 0 529 1098
Size: 529 569
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI
BstEII BtgI BtgZI EaeI EarI EcoICRI Eco57MI EcoO109I
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV PflMI PfoI PmlI PspOMI PstI PvuII
SacI SacII SapI SbfI SfcI SfoI SmaI SrfI
StuI StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BmtI BpuEI BsaI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BssHII BstBI BstXI
BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI
TstI Tth111I XbaI XhoI ZraI