Restriction map for
pFN21AE1116
(Linear) (Six-base) MAPSORT of: KIEE1116.seq Check: 6121 from: 1 to: 600
KIEE1116.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AvaI C'yCGr_G
Cuts at: 0 80 201 600
Size: 80 121 399
Fragments arranged by size:
399 121 80
BanII G_rGCy'C
Cuts at: 0 214 566 600
Size: 214 352 34
Fragments arranged by size:
352 214 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 26 350 600
Size: 26 324 250
Fragments arranged by size:
324 250 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 375 409 600
Size: 375 34 191
Fragments arranged by size:
375 191 34
BglI GCCn_nnn'nGGC
Cuts at: 0 412 600
Size: 412 188
BmrI ACTGGGnnnn_n'
Cuts at: 0 420 600
Size: 420 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 600
Size: 301 299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 550 600
Size: 13 537 50
Fragments arranged by size:
537 50 13
BsaAI yAC'GTr
Cuts at: 0 594 600
Size: 594 6
BsaWI w'CCGG_w
Cuts at: 0 494 600
Size: 494 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 268 298 600
Size: 268 30 302
Fragments arranged by size:
302 268 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 175 600
Size: 73 102 425
Fragments arranged by size:
425 102 73
BsiEI CG_ry'CG
Cuts at: 0 42 600
Size: 42 558
BsiHKAI G_wGCw'C
Cuts at: 0 266 600
Size: 266 334
Bsp1286I G_dGCh'C
Cuts at: 0 214 266 566 600
Size: 214 52 300 34
Fragments arranged by size:
300 214 52 34
BspEI T'CCGG_A
Cuts at: 0 494 600
Size: 494 106
BstBI TT'CG_AA
Cuts at: 0 16 600
Size: 16 584
BtgI C'CryG_G
Cuts at: 0 186 291 302 600
Size: 186 105 11 298
Fragments arranged by size:
298 186 105 11
BtsI GCAGTG_nn'
Cuts at: 0 329 436 600
Size: 329 107 164
Fragments arranged by size:
329 164 107
DrdI GACnn_nn'nnGTC
Cuts at: 0 105 600
Size: 105 495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 600
Size: 301 299
EcoNI CCTnn'n_nnAGG
Cuts at: 0 535 600
Size: 535 65
EcoO109I rG'GnC_Cy
Cuts at: 0 84 600
Size: 84 516
HaeII r_GCGC'y
Cuts at: 0 576 600
Size: 576 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 600
Size: 319 32 249
Fragments arranged by size:
319 249 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 197 600
Size: 62 135 403
Fragments arranged by size:
403 135 62
MspA1I CmG'CkG
Cuts at: 0 120 132 188 293 600
Size: 120 12 56 105 307
Fragments arranged by size:
307 120 105 56 12
NcoI C'CATG_G
Cuts at: 0 302 600
Size: 302 298
NspI r_CATG'y
Cuts at: 0 129 396 600
Size: 129 267 204
Fragments arranged by size:
267 204 129
PpuMI rG'GwC_Cy
Cuts at: 0 84 600
Size: 84 516
PstI C_TGCA'G
Cuts at: 0 548 600
Size: 548 52
PvuII CAG'CTG
Cuts at: 0 120 600
Size: 120 480
SacII CC_GC'GG
Cuts at: 0 189 294 600
Size: 189 105 306
Fragments arranged by size:
306 189 105
SfcI C'TryA_G
Cuts at: 0 544 600
Size: 544 56
SmaI CCC'GGG
Cuts at: 0 203 600
Size: 203 397
SmlI C'TyrA_G
Cuts at: 0 28 565 600
Size: 28 537 35
Fragments arranged by size:
537 35 28
SspI AAT'ATT
Cuts at: 0 381 600
Size: 381 219
StyI C'CwwG_G
Cuts at: 0 45 302 600
Size: 45 257 298
Fragments arranged by size:
298 257 45
XmaI C'CCGG_G
Cuts at: 0 201 600
Size: 201 399
Enzymes that do cut:
AvaI BanII BbsI BcgI BglI BmrI BpmI BpuEI
BsaAI BsaWI BsaXI BseRI BsiEI BsiHKAI Bsp1286I BspEI
BstBI BtgI BtsI DrdI Eco57MI EcoNI EcoO109I HaeII
Hin4I MmeI MspA1I NcoI NspI PpuMI PstI PvuII
SacII SfcI SmaI SmlI SspI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI
BseYI BsgI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EaeI EagI EarI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI