(Linear) (Six-base) MAPSORT of: KIEE1112.seq  Check: 8513  from: 1  to: 426



KIEE1112.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      82     114     426 
   Size:         82      32     312

  Fragments arranged by size: 

                 312      82      32
 
ApaLI G'TGCA_C

Cuts at:      0     364     426 
   Size:        364      62
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      58      92     256     290     426 
   Size:         58      34     164      34     136

  Fragments arranged by size: 

                 164     136      58      34      34
 
Bme1580I G_kGCm'C

Cuts at:      0     368     426 
   Size:        368      58
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     426 
   Size:        109     317
 
Bpu10I CC'TnA_GC

Cuts at:      0     145     250     304     426 
   Size:        145     105      54     122

  Fragments arranged by size: 

                 145     122     105      54
 
BsaAI yAC'GTr

Cuts at:      0     219     426 
   Size:        219     207
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      82     112     426 
   Size:         82      30     314

  Fragments arranged by size: 

                 314      82      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     426 
   Size:        244     182
 
BsiHKAI G_wGCw'C

Cuts at:      0     368     403     426 
   Size:        368      35      23

  Fragments arranged by size: 

                 368      35      23
 
Bsp1286I G_dGCh'C

Cuts at:      0     368     403     426 
   Size:        368      35      23

  Fragments arranged by size: 

                 368      35      23
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     266     426 
   Size:        266     160
 
BssHII G'CGCG_C

Cuts at:      0      56     426 
   Size:         56     370
 
BstEII G'GTnAC_C

Cuts at:      0     321     426 
   Size:        321     105
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     210     426 
   Size:        210     216
 
BtgI C'CryG_G

Cuts at:      0     150     174     209     426 
   Size:        150      24      35     217

  Fragments arranged by size: 

                 217     150      35      24
 
BtsI GCAGTG_nn'

Cuts at:      0     309     426 
   Size:        309     117
 
EaeI y'GGCC_r

Cuts at:      0     188     329     426 
   Size:        188     141      97

  Fragments arranged by size: 

                 188     141      97
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     234     426 
   Size:        234     192
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     426 
   Size:        109     317
 
EcoO109I rG'GnC_Cy

Cuts at:      0      84     426 
   Size:         84     342
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     144     426 
   Size:        144     282
 
HincII GTy'rAC

Cuts at:      0     291     426 
   Size:        291     135
 
HindIII A'AGCT_T

Cuts at:      0     271     426 
   Size:        271     155
 
NspI r_CATG'y

Cuts at:      0     231     426 
   Size:        231     195
 
PmlI CAC'GTG

Cuts at:      0     219     426 
   Size:        219     207
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      82     114     426 
   Size:         82      32     312

  Fragments arranged by size: 

                 312      82      32
 
PshAI GACnn'nnGTC

Cuts at:      0      28     426 
   Size:         28     398
 
Tth111I GACn'n_nGTC

Cuts at:      0     286     426 
   Size:        286     140
 

 Enzymes that do cut:

     AloI    ApaLI     BcgI Bme1580I     BpmI   Bpu10I    BsaAI    BsaXI
     BsgI  BsiHKAI Bsp1286I    BspMI   BssHII   BstEII    BstXI     BtgI
     BtsI     EaeI     EciI  Eco57MI EcoO109I     FalI   HincII  HindIII
     NspI     PmlI     PpiI    PshAI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI