(Linear) (Six-base) MAPSORT of: KIEE1111.seq  Check: 5079  from: 1  to: 390



KIEE1111.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

ApaLI G'TGCA_C

Cuts at:      0      91     390 
   Size:         91     299
 
AscI GG'CGCG_CC

Cuts at:      0     140     390 
   Size:        140     250
 
BanII G_rGCy'C

Cuts at:      0      71     281     390 
   Size:         71     210     109

  Fragments arranged by size: 

                 210     109      71
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     364     390 
   Size:        364      26
 
BlpI GC'TnA_GC

Cuts at:      0     122     390 
   Size:        122     268
 
Bme1580I G_kGCm'C

Cuts at:      0      95     390 
   Size:         95     295
 
BmgBI CAC'GTC

Cuts at:      0      10     390 
   Size:         10     380
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      85     390 
   Size:         85     305
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     211     390 
   Size:        187      24     179

  Fragments arranged by size: 

                 187     179      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     311     380     390 
   Size:        311      69      10

  Fragments arranged by size: 

                 311      69      10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      45     289     390 
   Size:         45     244     101

  Fragments arranged by size: 

                 244     101      45
 
BseYI C'CCAG_C

Cuts at:      0      64     390 
   Size:         64     326
 
BsiHKAI G_wGCw'C

Cuts at:      0      95     281     390 
   Size:         95     186     109

  Fragments arranged by size: 

                 186     109      95
 
Bsp1286I G_dGCh'C

Cuts at:      0      71      95     281     390 
   Size:         71      24     186     109

  Fragments arranged by size: 

                 186     109      71      24
 
BsrBI CCG'CTC

Cuts at:      0      53     126     390 
   Size:         53      73     264

  Fragments arranged by size: 

                 264      73      53
 
BsrFI r'CCGG_y

Cuts at:      0     136     144     390 
   Size:        136       8     246

  Fragments arranged by size: 

                 246     136       8
 
BssHII G'CGCG_C

Cuts at:      0     140     390 
   Size:        140     250
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      99     390 
   Size:         99     291
 
BstYI r'GATC_y

Cuts at:      0     185     390 
   Size:        185     205
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     291     390 
   Size:        291      99
 
EaeI y'GGCC_r

Cuts at:      0     257     390 
   Size:        257     133
 
EcoICRI GAG'CTC

Cuts at:      0     279     390 
   Size:        279     111
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     211     390 
   Size:        187      24     179

  Fragments arranged by size: 

                 187     179      24
 
MscI TGG'CCA

Cuts at:      0     259     390 
   Size:        259     131
 
MspA1I CmG'CkG

Cuts at:      0     255     390 
   Size:        255     135
 
NaeI GCC'GGC

Cuts at:      0     138     390 
   Size:        138     252
 
NgoMIV G'CCGG_C

Cuts at:      0     136     390 
   Size:        136     254
 
PfoI T'CCnGG_A

Cuts at:      0     188     390 
   Size:        188     202
 
PvuII CAG'CTG

Cuts at:      0     255     390 
   Size:        255     135
 
SacI G_AGCT'C

Cuts at:      0     281     390 
   Size:        281     109
 
SfcI C'TryA_G

Cuts at:      0      80     390 
   Size:         80     310
 
StyI C'CwwG_G

Cuts at:      0      38     390 
   Size:         38     352
 
XbaI T'CTAG_A

Cuts at:      0      58     390 
   Size:         58     332
 

 Enzymes that do cut:

    ApaLI     AscI    BanII     BbsI     BlpI Bme1580I    BmgBI     BplI
     BpmI   Bpu10I    BseRI    BseYI  BsiHKAI Bsp1286I    BsrBI    BsrFI
   BssHII   BstAPI    BstYI    BtgZI     EaeI  EcoICRI  Eco57MI     MscI
   MspA1I     NaeI   NgoMIV     PfoI    PvuII     SacI     SfcI     StyI
     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     ApoI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrGI    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI