(Linear) (Six-base) MAPSORT of: KIEE1109.seq  Check: 5387  from: 1  to: 333



KIEE1109.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     301     333 
   Size:        301      32
 
AvrII C'CTAG_G

Cuts at:      0      31     333 
   Size:         31     302
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     245     278     333 
   Size:        245      33      55

  Fragments arranged by size: 

                 245      55      33
 
BclI T'GATC_A

Cuts at:      0     144     162     333 
   Size:        144      18     171

  Fragments arranged by size: 

                 171     144      18
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     242     333 
   Size:        242      91
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     333 
   Size:        193     140
 
Bpu10I CC'TnA_GC

Cuts at:      0      59     333 
   Size:         59     274
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     333 
   Size:        191     142
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     301     333 
   Size:        301      32
 
DraI TTT'AAA

Cuts at:      0     297     333 
   Size:        297      36
 
EarI CTCTTCn'nnn_

Cuts at:      0      89     101     145     333 
   Size:         89      12      44     188

  Fragments arranged by size: 

                 188      89      44      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     333 
   Size:        193     140
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     333 
   Size:        109     224
 
MspA1I CmG'CkG

Cuts at:      0     175     333 
   Size:        175     158
 
PfoI T'CCnGG_A

Cuts at:      0     209     333 
   Size:        209     124
 
PvuII CAG'CTG

Cuts at:      0     175     333 
   Size:        175     158
 
SapI GCTCTTCn'nnn_

Cuts at:      0      89     333 
   Size:         89     244
 
StuI AGG'CCT

Cuts at:      0      30     333 
   Size:         30     303
 
StyI C'CwwG_G

Cuts at:      0      31     333 
   Size:         31     302
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     132     333 
   Size:        132     201
 

 Enzymes that do cut:

     AarI    AvrII     BaeI     BclI     BmrI     BpmI   Bpu10I     BsgI
    BspMI     DraI     EarI  Eco57MI     MmeI   MspA1I     PfoI    PvuII
     SapI     StuI     StyI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI