Restriction map for
pFN21AE1094
(Linear) (Six-base) MAPSORT of: KIEE1094.seq Check: 7272 from: 1 to: 2733
KIEE1094.orf
With 182 enzymes: SgfI *
December 27, 2011 16:31 ..
AccI GT'mk_AC
Cuts at: 0 1640 2733
Size: 1640 1093
AclI AA'CG_TT
Cuts at: 0 1952 2733
Size: 1952 781
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 941 2341 2387 2733
Size: 99 842 1400 46 346
Fragments arranged by size:
1400 842 346 99 46
AflIII A'CryG_T
Cuts at: 0 2087 2733
Size: 2087 646
AlwNI CAG_nnn'CTG
Cuts at: 0 2110 2733
Size: 2110 623
ApoI r'AATT_y
Cuts at: 0 121 142 1961 2050 2346 2733
Size: 121 21 1819 89 296 387
Fragments arranged by size:
1819 387 296 121 89 21
AseI AT'TA_AT
Cuts at: 0 2477 2733
Size: 2477 256
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2472 2505 2733
Size: 2472 33 228
Fragments arranged by size:
2472 228 33
BbvCI CC'TCA_GC
Cuts at: 0 218 2570 2733
Size: 218 2352 163
Fragments arranged by size:
2352 218 163
BclI T'GATC_A
Cuts at: 0 566 897 2733
Size: 566 331 1836
Fragments arranged by size:
1836 566 331
BfrBI ATG'CAT
Cuts at: 0 952 1249 2733
Size: 952 297 1484
Fragments arranged by size:
1484 952 297
BglI GCCn_nnn'nGGC
Cuts at: 0 2172 2733
Size: 2172 561
BglII A'GATC_T
Cuts at: 0 657 2733
Size: 657 2076
BlpI GC'TnA_GC
Cuts at: 0 269 2733
Size: 269 2464
Bme1580I G_kGCm'C
Cuts at: 0 2242 2733
Size: 2242 491
BmgBI CAC'GTC
Cuts at: 0 94 2733
Size: 94 2639
BmrI ACTGGGnnnn_n'
Cuts at: 0 1691 2733
Size: 1691 1042
BmtI G_CTAG'C
Cuts at: 0 751 2733
Size: 751 1982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 2053 2733
Size: 386 1667 680
Fragments arranged by size:
1667 680 386
Bpu10I CC'TnA_GC
Cuts at: 0 218 2570 2733
Size: 218 2352 163
Fragments arranged by size:
2352 218 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 2733
Size: 562 2171
BsaAI yAC'GTr
Cuts at: 0 2090 2733
Size: 2090 643
BsaBI GATnn'nnATC
Cuts at: 0 1073 2733
Size: 1073 1660
BsaHI Gr'CG_yC
Cuts at: 0 2442 2733
Size: 2442 291
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2521 2537 2551 2567 2733
Size: 2521 16 14 16 166
Fragments arranged by size:
2521 166 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2414 2733
Size: 2414 319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 1228 1475 1512 1704 2733
Size: 116 1112 247 37 192 1029
Fragments arranged by size:
1112 1029 247 192 116 37
BsiHKAI G_wGCw'C
Cuts at: 0 773 1928 2733
Size: 773 1155 805
Fragments arranged by size:
1155 805 773
BsmI GAATG_Cn'
Cuts at: 0 952 2733
Size: 952 1781
Bsp1286I G_dGCh'C
Cuts at: 0 773 1928 2242 2733
Size: 773 1155 314 491
Fragments arranged by size:
1155 773 491 314
BspHI T'CATG_A
Cuts at: 0 147 2733
Size: 147 2586
BsrDI GCAATG_nn'
Cuts at: 0 206 383 2733
Size: 206 177 2350
Fragments arranged by size:
2350 206 177
BssSI C'ACGA_G
Cuts at: 0 1511 2733
Size: 1511 1222
BstAPI GCAn_nnn'nTGC
Cuts at: 0 382 2733
Size: 382 2351
BstYI r'GATC_y
Cuts at: 0 657 2733
Size: 657 2076
BstZ17I GTA'TAC
Cuts at: 0 1641 2733
Size: 1641 1092
BtgI C'CryG_G
Cuts at: 0 2209 2733
Size: 2209 524
DraI TTT'AAA
Cuts at: 0 329 581 738 2625 2658 2733
Size: 329 252 157 1887 33 75
Fragments arranged by size:
1887 329 252 157 75 33
DraIII CAC_nnn'GTG
Cuts at: 0 1429 2733
Size: 1429 1304
EaeI y'GGCC_r
Cuts at: 0 1565 2733
Size: 1565 1168
EarI CTCTTCn'nnn_
Cuts at: 0 161 1271 2733
Size: 161 1110 1462
Fragments arranged by size:
1462 1110 161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 386 941 2053 2341 2387 2733
Size: 99 287 555 1112 288 46 346
Fragments arranged by size:
1112 555 346 288 287 99 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 695 920 2733
Size: 695 225 1813
Fragments arranged by size:
1813 695 225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 946 2733
Size: 946 1787
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1474 1506 2563 2595 2733
Size: 1474 32 1057 32 138
Fragments arranged by size:
1474 1057 138 32 32
HincII GTy'rAC
Cuts at: 0 1956 2733
Size: 1956 777
HindIII A'AGCT_T
Cuts at: 0 271 583 2199 2627 2733
Size: 271 312 1616 428 106
Fragments arranged by size:
1616 428 312 271 106
HpaI GTT'AAC
Cuts at: 0 1956 2733
Size: 1956 777
MfeI C'AATT_G
Cuts at: 0 461 2733
Size: 461 2272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1380 2733
Size: 1380 1353
MscI TGG'CCA
Cuts at: 0 1567 2733
Size: 1567 1166
MslI CAynn'nnrTG
Cuts at: 0 173 359 626 848 2122 2609 2733
Size: 173 186 267 222 1274 487 124
Fragments arranged by size:
1274 487 267 222 186 173 124
NcoI C'CATG_G
Cuts at: 0 2209 2733
Size: 2209 524
NdeI CA'TA_TG
Cuts at: 0 2234 2733
Size: 2234 499
NheI G'CTAG_C
Cuts at: 0 747 2733
Size: 747 1986
NsiI A_TGCA'T
Cuts at: 0 954 1251 2733
Size: 954 297 1482
Fragments arranged by size:
1482 954 297
PflMI CCAn_nnn'nTGG
Cuts at: 0 1574 2733
Size: 1574 1159
PsiI TTA'TAA
Cuts at: 0 523 2733
Size: 523 2210
SapI GCTCTTCn'nnn_
Cuts at: 0 161 2733
Size: 161 2572
SfcI C'TryA_G
Cuts at: 0 333 653 672 695 2733
Size: 333 320 19 23 2038
Fragments arranged by size:
2038 333 320 23 19
SmlI C'TyrA_G
Cuts at: 0 541 2733
Size: 541 2192
StyI C'CwwG_G
Cuts at: 0 2209 2525 2733
Size: 2209 316 208
Fragments arranged by size:
2209 316 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 357 2733
Size: 357 2376
TatI w'GTAC_w
Cuts at: 0 2067 2733
Size: 2067 666
Enzymes that do cut:
AccI AclI AcuI AflIII AlwNI ApoI AseI BaeI
BbvCI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI
BssSI BstAPI BstYI BstZ17I BtgI DraI DraIII EaeI
EarI Eco57MI EcoNI FalI Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI NcoI NdeI NheI NsiI
PflMI PsiI SapI SfcI SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BplI
BsaI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BstBI BstEII BstXI Bsu36I
BtgZI BtsI ClaI CspCI DrdI EagI EciI EcoICRI
EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII KasI
KpnI MluI MspA1I NaeI NarI NgoMIV NotI NruI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI