Restriction map for
pFN21AE1091
(Linear) (Six-base) MAPSORT of: KIEE1091.seq Check: 3675 from: 1 to: 984
KIEE1091.orf
With 182 enzymes: SgfI *
October 13, 2009 15:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 361 984
Size: 361 623
AccI GT'mk_AC
Cuts at: 0 56 984
Size: 56 928
AfeI AGC'GCT
Cuts at: 0 370 984
Size: 370 614
AleI CACnn'nnGTG
Cuts at: 0 184 410 984
Size: 184 226 574
Fragments arranged by size:
574 226 184
AlwNI CAG_nnn'CTG
Cuts at: 0 30 477 682 984
Size: 30 447 205 302
Fragments arranged by size:
447 302 205 30
ApaI G_GGCC'C
Cuts at: 0 171 984
Size: 171 813
BanII G_rGCy'C
Cuts at: 0 171 944 984
Size: 171 773 40
Fragments arranged by size:
773 171 40
BbvCI CC'TCA_GC
Cuts at: 0 538 984
Size: 538 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 779 813 984
Size: 779 34 171
Fragments arranged by size:
779 171 34
BclI T'GATC_A
Cuts at: 0 267 984
Size: 267 717
BglI GCCn_nnn'nGGC
Cuts at: 0 625 668 718 787 984
Size: 625 43 50 69 197
Fragments arranged by size:
625 197 69 50 43
BglII A'GATC_T
Cuts at: 0 149 984
Size: 149 835
Bme1580I G_kGCm'C
Cuts at: 0 171 984
Size: 171 813
BmgBI CAC'GTC
Cuts at: 0 158 984
Size: 158 826
BmrI ACTGGGnnnn_n'
Cuts at: 0 465 984
Size: 465 519
Bpu10I CC'TnA_GC
Cuts at: 0 538 984
Size: 538 446
BsaHI Gr'CG_yC
Cuts at: 0 802 809 984
Size: 802 7 175
Fragments arranged by size:
802 175 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 926 956 984
Size: 926 30 28
Fragments arranged by size:
926 30 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 313 801 984
Size: 138 175 488 183
Fragments arranged by size:
488 183 175 138
BsiEI CG_ry'CG
Cuts at: 0 185 862 984
Size: 185 677 122
Fragments arranged by size:
677 185 122
BsiHKAI G_wGCw'C
Cuts at: 0 944 984
Size: 944 40
Bsp1286I G_dGCh'C
Cuts at: 0 171 944 984
Size: 171 773 40
Fragments arranged by size:
773 171 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 335 361 984
Size: 224 111 26 623
Fragments arranged by size:
623 224 111 26
BsrBI CCG'CTC
Cuts at: 0 696 984
Size: 696 288
BsrFI r'CCGG_y
Cuts at: 0 926 984
Size: 926 58
BsrGI T'GTAC_A
Cuts at: 0 188 984
Size: 188 796
BstAPI GCAn_nnn'nTGC
Cuts at: 0 81 527 984
Size: 81 446 457
Fragments arranged by size:
457 446 81
BstYI r'GATC_y
Cuts at: 0 149 984
Size: 149 835
BtgI C'CryG_G
Cuts at: 0 722 984
Size: 722 262
BtsI GCAGTG_nn'
Cuts at: 0 797 984
Size: 797 187
EaeI y'GGCC_r
Cuts at: 0 182 458 554 719 859 984
Size: 182 276 96 165 140 125
Fragments arranged by size:
276 182 165 140 125 96
EagI C'GGCC_G
Cuts at: 0 182 859 984
Size: 182 677 125
Fragments arranged by size:
677 182 125
EarI CTCTTCn'nnn_
Cuts at: 0 319 349 904 984
Size: 319 30 555 80
Fragments arranged by size:
555 319 80 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 913 984
Size: 913 71
EcoICRI GAG'CTC
Cuts at: 0 942 984
Size: 942 42
EcoO109I rG'GnC_Cy
Cuts at: 0 168 984
Size: 168 816
HaeII r_GCGC'y
Cuts at: 0 372 984
Size: 372 612
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 963 984
Size: 963 21
MscI TGG'CCA
Cuts at: 0 556 984
Size: 556 428
MslI CAynn'nnrTG
Cuts at: 0 184 410 721 984
Size: 184 226 311 263
Fragments arranged by size:
311 263 226 184
MspA1I CmG'CkG
Cuts at: 0 126 393 841 984
Size: 126 267 448 143
Fragments arranged by size:
448 267 143 126
NaeI GCC'GGC
Cuts at: 0 928 984
Size: 928 56
NgoMIV G'CCGG_C
Cuts at: 0 926 984
Size: 926 58
PflMI CCAn_nnn'nTGG
Cuts at: 0 30 393 984
Size: 30 363 591
Fragments arranged by size:
591 363 30
PspOMI G'GGCC_C
Cuts at: 0 167 984
Size: 167 817
SacI G_AGCT'C
Cuts at: 0 944 984
Size: 944 40
SfcI C'TryA_G
Cuts at: 0 946 984
Size: 946 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 718 984
Size: 718 266
SgrAI Cr'CCGG_yG
Cuts at: 0 926 984
Size: 926 58
StuI AGG'CCT
Cuts at: 0 832 984
Size: 832 152
StyI C'CwwG_G
Cuts at: 0 261 984
Size: 261 723
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 57 574 925 984
Size: 57 517 351 59
Fragments arranged by size:
517 351 59 57
TatI w'GTAC_w
Cuts at: 0 188 984
Size: 188 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 926 958 984
Size: 926 32 26
Fragments arranged by size:
926 32 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 697 882 984
Size: 697 185 102
Fragments arranged by size:
697 185 102
XmnI GAAnn'nnTTC
Cuts at: 0 919 984
Size: 919 65
Enzymes that do cut:
AarI AccI AfeI AleI AlwNI ApaI BanII BbvCI
BcgI BclI BglI BglII Bme1580I BmgBI BmrI Bpu10I
BsaHI BsaXI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BsrGI BstAPI BstYI BtgI BtsI EaeI EagI
EarI EciI EcoICRI EcoO109I HaeII Hin4I MscI MslI
MspA1I NaeI NgoMIV PflMI PspOMI SacI SfcI SfiI
SgrAI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BciVI BfrBI
BlpI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaWI BseRI BseYI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI ZraI