(Linear) (Six-base) MAPSORT of: KIEE1084.seq  Check: 7886  from: 1  to: 636



KIEE1084.orf

 With 182 enzymes: SgfI * 

                        June 30, 2009 14:14  ..

AccI GT'mk_AC

Cuts at:      0     371     636 
   Size:        371     265
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     636 
   Size:        131     505
 
AflIII A'CryG_T

Cuts at:      0     211     227     636 
   Size:        211      16     409

  Fragments arranged by size: 

                 409     211      16
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27     636 
   Size:         27     609
 
AlwNI CAG_nnn'CTG

Cuts at:      0     459     555     636 
   Size:        459      96      81

  Fragments arranged by size: 

                 459      96      81
 
ApoI r'AATT_y

Cuts at:      0     167     636 
   Size:        167     469
 
BanII G_rGCy'C

Cuts at:      0     624     636 
   Size:        624      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      79     636 
   Size:         79     557
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     636 
   Size:        370     266
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25     636 
   Size:         25     611
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     337     636 
   Size:        337     299
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     636 
   Size:        448     188
 
Bsp1286I G_dGCh'C

Cuts at:      0     624     636 
   Size:        624      12
 
BstZ17I GTA'TAC

Cuts at:      0     372     636 
   Size:        372     264
 
Bsu36I CC'TnA_GG

Cuts at:      0     616     636 
   Size:        616      20
 
EarI CTCTTCn'nnn_

Cuts at:      0     150     629     636 
   Size:        150     479       7

  Fragments arranged by size: 

                 479     150       7
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     370     636 
   Size:        131     239     266

  Fragments arranged by size: 

                 266     239     131
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     202     636 
   Size:        202     434
 
FspI TGC'GCA

Cuts at:      0     586     636 
   Size:        586      50
 
HaeII r_GCGC'y

Cuts at:      0      23     636 
   Size:         23     613
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     122     154     175     207     558     590     636 
   Size:        122      32      21      32     351      32      46

  Fragments arranged by size: 

                 351     122      46      32      32      32      21
 
NspI r_CATG'y

Cuts at:      0     215     231     636 
   Size:        215      16     405

  Fragments arranged by size: 

                 405     215      16
 
PasI CC'CwG_GG

Cuts at:      0      45     636 
   Size:         45     591
 
PciI A'CATG_T

Cuts at:      0     211     227     636 
   Size:        211      16     409

  Fragments arranged by size: 

                 409     211      16
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      27     636 
   Size:         27     609
 
PstI C_TGCA'G

Cuts at:      0     464     539     560     608     636 
   Size:        464      75      21      48      28

  Fragments arranged by size: 

                 464      75      48      28      21
 
SapI GCTCTTCn'nnn_

Cuts at:      0     629     636 
   Size:        629       7
 
SfcI C'TryA_G

Cuts at:      0     460     535     556     604     636 
   Size:        460      75      21      48      32

  Fragments arranged by size: 

                 460      75      48      32      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      16     636 
   Size:         16     620
 
TatI w'GTAC_w

Cuts at:      0     436     636 
   Size:        436     200
 
XbaI T'CTAG_A

Cuts at:      0     480     636 
   Size:        480     156
 
XmnI GAAnn'nnTTC

Cuts at:      0       7     636 
   Size:          7     629
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AloI    AlwNI     ApoI    BanII     BmrI
     BpmI    BsaXI    BseRI     BsgI Bsp1286I  BstZ17I   Bsu36I     EarI
  Eco57MI    EcoNI     FspI    HaeII    Hin4I     NspI     PasI     PciI
     PpiI     PstI     SapI     SfcI    TaqII     TatI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I
     XcmI     XhoI     XmaI     ZraI