Restriction map for
pFN21AE1053
(Linear) (Six-base) MAPSORT of: KIEE1053.seq Check: 1106 from: 1 to: 1128
KIEE1053.orf
With 182 enzymes: SgfI *
March 2, 2010 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 779 1031 1128
Size: 779 252 97
Fragments arranged by size:
779 252 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 726 810 1128
Size: 616 110 84 318
Fragments arranged by size:
616 318 110 84
AfeI AGC'GCT
Cuts at: 0 655 1128
Size: 655 473
AleI CACnn'nnGTG
Cuts at: 0 869 1128
Size: 869 259
AlwNI CAG_nnn'CTG
Cuts at: 0 457 771 861 1128
Size: 457 314 90 267
Fragments arranged by size:
457 314 267 90
ApaI G_GGCC'C
Cuts at: 0 333 464 1128
Size: 333 131 664
Fragments arranged by size:
664 333 131
ApaLI G'TGCA_C
Cuts at: 0 1114 1128
Size: 1114 14
AvaI C'yCGr_G
Cuts at: 0 463 475 1128
Size: 463 12 653
Fragments arranged by size:
653 463 12
AvrII C'CTAG_G
Cuts at: 0 9 1128
Size: 9 1119
BanI G'GyrC_C
Cuts at: 0 421 1019 1060 1128
Size: 421 598 41 68
Fragments arranged by size:
598 421 68 41
BanII G_rGCy'C
Cuts at: 0 233 333 464 838 922 1090 1128
Size: 233 100 131 374 84 168 38
Fragments arranged by size:
374 233 168 131 100 84 38
BbeI G_GCGC'C
Cuts at: 0 425 1064 1128
Size: 425 639 64
Fragments arranged by size:
639 425 64
BbvCI CC'TCA_GC
Cuts at: 0 95 1128
Size: 95 1033
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 466 500 1059 1093 1128
Size: 466 34 559 34 35
Fragments arranged by size:
559 466 35 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 521 731 899 1128
Size: 521 210 168 229
Fragments arranged by size:
521 229 210 168
Bme1580I G_kGCm'C
Cuts at: 0 333 464 1118 1128
Size: 333 131 654 10
Fragments arranged by size:
654 333 131 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 936 1128
Size: 120 816 192
Fragments arranged by size:
816 192 120
Bpu10I CC'TnA_GC
Cuts at: 0 95 1128
Size: 95 1033
BsaHI Gr'CG_yC
Cuts at: 0 422 1061 1128
Size: 422 639 67
Fragments arranged by size:
639 422 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 958 988 1128
Size: 958 30 140
Fragments arranged by size:
958 140 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 327 1128
Size: 327 801
BseYI C'CCAG_C
Cuts at: 0 80 155 410 457 492 1091 1128
Size: 80 75 255 47 35 599 37
Fragments arranged by size:
599 255 80 75 47 37 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 463 847 1128
Size: 357 106 384 281
Fragments arranged by size:
384 357 281 106
BsiEI CG_ry'CG
Cuts at: 0 376 510 735 1002 1128
Size: 376 134 225 267 126
Fragments arranged by size:
376 267 225 134 126
BsiHKAI G_wGCw'C
Cuts at: 0 152 838 922 929 938 976 1090 1118
Size: 152 686 84 7 9 38 114 28
Cuts at: 1118 1128
Size: 10
Fragments arranged by size:
686 152 114 84 38 28 10 9
7
Bsp1286I G_dGCh'C
Cuts at: 0 152 233 333 464 838 922 929 938
Size: 152 81 100 131 374 84 7 9
Cuts at: 938 976 1090 1118 1128
Size: 38 114 28 10
Fragments arranged by size:
374 152 131 114 100 84 81 38
28 10 9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 779 1031 1128
Size: 779 252 97
Fragments arranged by size:
779 252 97
BsrBI CCG'CTC
Cuts at: 0 372 480 1004 1128
Size: 372 108 524 124
Fragments arranged by size:
524 372 124 108
BsrFI r'CCGG_y
Cuts at: 0 343 375 515 1128
Size: 343 32 140 613
Fragments arranged by size:
613 343 140 32
BssHII G'CGCG_C
Cuts at: 0 527 1128
Size: 527 601
BssSI C'ACGA_G
Cuts at: 0 88 439 640 1128
Size: 88 351 201 488
Fragments arranged by size:
488 351 201 88
BstAPI GCAn_nnn'nTGC
Cuts at: 0 771 896 1128
Size: 771 125 232
Fragments arranged by size:
771 232 125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 391 1128
Size: 391 737
BstYI r'GATC_y
Cuts at: 0 171 1128
Size: 171 957
Bsu36I CC'TnA_GG
Cuts at: 0 143 310 1128
Size: 143 167 818
Fragments arranged by size:
818 167 143
BtgI C'CryG_G
Cuts at: 0 108 415 660 996 1128
Size: 108 307 245 336 132
Fragments arranged by size:
336 307 245 132 108
EaeI y'GGCC_r
Cuts at: 0 373 723 732 816 999 1080 1128
Size: 373 350 9 84 183 81 48
Fragments arranged by size:
373 350 183 84 81 48 9
EagI C'GGCC_G
Cuts at: 0 373 732 999 1128
Size: 373 359 267 129
Fragments arranged by size:
373 359 267 129
EcoICRI GAG'CTC
Cuts at: 0 836 920 1088 1128
Size: 836 84 168 40
Fragments arranged by size:
836 168 84 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 616 726 810 936 1128
Size: 120 496 110 84 126 192
Fragments arranged by size:
496 192 126 120 110 84
EcoO109I rG'GnC_Cy
Cuts at: 0 270 329 1128
Size: 270 59 799
Fragments arranged by size:
799 270 59
FspI TGC'GCA
Cuts at: 0 301 775 1128
Size: 301 474 353
Fragments arranged by size:
474 353 301
HaeII r_GCGC'y
Cuts at: 0 298 344 425 657 1064 1128
Size: 298 46 81 232 407 64
Fragments arranged by size:
407 298 232 81 64 46
HincII GTy'rAC
Cuts at: 0 501 1128
Size: 501 627
KasI G'GCGC_C
Cuts at: 0 421 1060 1128
Size: 421 639 68
Fragments arranged by size:
639 421 68
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 490 863 1001 1128
Size: 188 302 373 138 127
Fragments arranged by size:
373 302 188 138 127
MslI CAynn'nnrTG
Cuts at: 0 107 119 869 1128
Size: 107 12 750 259
Fragments arranged by size:
750 259 107 12
MspA1I CmG'CkG
Cuts at: 0 417 454 662 858 998 1110 1128
Size: 417 37 208 196 140 112 18
Fragments arranged by size:
417 208 196 140 112 37 18
NaeI GCC'GGC
Cuts at: 0 345 377 517 1128
Size: 345 32 140 611
Fragments arranged by size:
611 345 140 32
NarI GG'CG_CC
Cuts at: 0 422 1061 1128
Size: 422 639 67
Fragments arranged by size:
639 422 67
NcoI C'CATG_G
Cuts at: 0 108 1128
Size: 108 1020
NgoMIV G'CCGG_C
Cuts at: 0 343 375 515 1128
Size: 343 32 140 613
Fragments arranged by size:
613 343 140 32
NotI GC'GGCC_GC
Cuts at: 0 732 999 1128
Size: 732 267 129
Fragments arranged by size:
732 267 129
PspOMI G'GGCC_C
Cuts at: 0 329 460 1128
Size: 329 131 668
Fragments arranged by size:
668 329 131
PstI C_TGCA'G
Cuts at: 0 892 1128
Size: 892 236
SacI G_AGCT'C
Cuts at: 0 838 922 1090 1128
Size: 838 84 168 38
Fragments arranged by size:
838 168 84 38
SacII CC_GC'GG
Cuts at: 0 418 663 999 1128
Size: 418 245 336 129
Fragments arranged by size:
418 336 245 129
SexAI A'CCwGG_T
Cuts at: 0 842 1128
Size: 842 286
SfcI C'TryA_G
Cuts at: 0 888 1128
Size: 888 240
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 731 1128
Size: 731 397
SfoI GGC'GCC
Cuts at: 0 423 1062 1128
Size: 423 639 66
Fragments arranged by size:
639 423 66
StuI AGG'CCT
Cuts at: 0 559 823 907 1075 1128
Size: 559 264 84 168 53
Fragments arranged by size:
559 264 168 84 53
StyI C'CwwG_G
Cuts at: 0 9 108 1128
Size: 9 99 1020
Fragments arranged by size:
1020 99 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 496 1128
Size: 496 632
XmnI GAAnn'nnTTC
Cuts at: 0 1048 1128
Size: 1048 80
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI ApaLI AvaI
AvrII BanI BanII BbeI BbvCI BcgI BglI Bme1580I
BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstXI BstYI Bsu36I BtgI EaeI EagI EcoICRI Eco57MI
EcoO109I FspI HaeII HincII KasI MmeI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI PspOMI PstI SacI
SacII SexAI SfcI SfiI SfoI StuI StyI TaqII
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI BaeI BamHI
BbsI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BstBI BstEII
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoNI EcoRI EcoRV FalI FseI FspAI
Hin4I HindIII HpaI KpnI MfeI MluI MscI NdeI
NheI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SalI SanDI SapI SbfI
ScaI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI