Restriction map for
pFN21AE0995
(Linear) (Six-base) MAPSORT of: KIEE0995.seq Check: 2735 from: 1 to: 1944
KIEE0995.orf
With 182 enzymes: SgfI *
July 3, 2012 16:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1869 1944
Size: 1869 75
AccI GT'mk_AC
Cuts at: 0 1259 1787 1944
Size: 1259 528 157
Fragments arranged by size:
1259 528 157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 427 1807 1944
Size: 43 384 1380 137
Fragments arranged by size:
1380 384 137 43
AfeI AGC'GCT
Cuts at: 0 1801 1944
Size: 1801 143
AflIII A'CryG_T
Cuts at: 0 1604 1944
Size: 1604 340
AleI CACnn'nnGTG
Cuts at: 0 95 158 1944
Size: 95 63 1786
Fragments arranged by size:
1786 95 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 113 1160 1944
Size: 113 1047 784
Fragments arranged by size:
1047 784 113
AlwNI CAG_nnn'CTG
Cuts at: 0 453 545 1944
Size: 453 92 1399
Fragments arranged by size:
1399 453 92
ApaI G_GGCC'C
Cuts at: 0 75 508 1944
Size: 75 433 1436
Fragments arranged by size:
1436 433 75
ApoI r'AATT_y
Cuts at: 0 1568 1944
Size: 1568 376
AvaI C'yCGr_G
Cuts at: 0 68 238 396 559 869 1944
Size: 68 170 158 163 310 1075
Fragments arranged by size:
1075 310 170 163 158 68
BanI G'GyrC_C
Cuts at: 0 221 1121 1944
Size: 221 900 823
Fragments arranged by size:
900 823 221
BanII G_rGCy'C
Cuts at: 0 75 508 974 1355 1688 1944
Size: 75 433 466 381 333 256
Fragments arranged by size:
466 433 381 333 256 75
BbvCI CC'TCA_GC
Cuts at: 0 115 640 769 1944
Size: 115 525 129 1175
Fragments arranged by size:
1175 525 129 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1374 1408 1944
Size: 1374 34 536
Fragments arranged by size:
1374 536 34
BclI T'GATC_A
Cuts at: 0 1211 1370 1944
Size: 1211 159 574
Fragments arranged by size:
1211 574 159
BglI GCCn_nnn'nGGC
Cuts at: 0 1005 1944
Size: 1005 939
BglII A'GATC_T
Cuts at: 0 1205 1761 1944
Size: 1205 556 183
Fragments arranged by size:
1205 556 183
Bme1580I G_kGCm'C
Cuts at: 0 75 102 508 1944
Size: 75 27 406 1436
Fragments arranged by size:
1436 406 75 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 305 1743 1944
Size: 305 1438 201
Fragments arranged by size:
1438 305 201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 488 1244 1944
Size: 488 756 700
Fragments arranged by size:
756 700 488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 938 1312 1615 1944
Size: 475 463 374 303 329
Fragments arranged by size:
475 463 374 329 303
Bpu10I CC'TnA_GC
Cuts at: 0 115 640 707 769 1944
Size: 115 525 67 62 1175
Fragments arranged by size:
1175 525 115 67 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1159 1417 1944
Size: 617 542 258 527
Fragments arranged by size:
617 542 527 258
BsaI GGTCTCn'nnnn_
Cuts at: 0 143 160 166 560 639 1123 1304 1515
Size: 143 17 6 394 79 484 181 211
Cuts at: 1515 1944
Size: 429
Fragments arranged by size:
484 429 394 211 181 143 79 17
6
BsaAI yAC'GTr
Cuts at: 0 1299 1557 1944
Size: 1299 258 387
Fragments arranged by size:
1299 387 258
BsaBI GATnn'nnATC
Cuts at: 0 1153 1210 1944
Size: 1153 57 734
Fragments arranged by size:
1153 734 57
BsaHI Gr'CG_yC
Cuts at: 0 1322 1944
Size: 1322 622
BsaWI w'CCGG_w
Cuts at: 0 1907 1944
Size: 1907 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 574 604 1944
Size: 574 30 1340
Fragments arranged by size:
1340 574 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 132 256 657 775 887 1322 1944
Size: 132 124 401 118 112 435 622
Fragments arranged by size:
622 435 401 132 124 118 112
BseYI C'CCAG_C
Cuts at: 0 86 649 1009 1704 1888 1944
Size: 86 563 360 695 184 56
Fragments arranged by size:
695 563 360 184 86 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1321 1944
Size: 1321 623
BsiEI CG_ry'CG
Cuts at: 0 1017 1944
Size: 1017 927
BsiHKAI G_wGCw'C
Cuts at: 0 839 1688 1944
Size: 839 849 256
Fragments arranged by size:
849 839 256
BsmI GAATG_Cn'
Cuts at: 0 29 1944
Size: 29 1915
Bsp1286I G_dGCh'C
Cuts at: 0 75 102 508 839 974 1355 1688 1944
Size: 75 27 406 331 135 381 333 256
Fragments arranged by size:
406 381 333 331 256 135 75 27
BspEI T'CCGG_A
Cuts at: 0 1907 1944
Size: 1907 37
BspHI T'CATG_A
Cuts at: 0 1367 1944
Size: 1367 577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 960 1869 1926 1944
Size: 960 909 57 18
Fragments arranged by size:
960 909 57 18
BsrBI CCG'CTC
Cuts at: 0 1005 1944
Size: 1005 939
BsrDI GCAATG_nn'
Cuts at: 0 1040 1944
Size: 1040 904
BsrFI r'CCGG_y
Cuts at: 0 1772 1944
Size: 1772 172
BstAPI GCAn_nnn'nTGC
Cuts at: 0 571 1944
Size: 571 1373
BstXI CCAn_nnnn'nTGG
Cuts at: 0 160 489 1944
Size: 160 329 1455
Fragments arranged by size:
1455 329 160
BstYI r'GATC_y
Cuts at: 0 233 1205 1761 1944
Size: 233 972 556 183
Fragments arranged by size:
972 556 233 183
BtgI C'CryG_G
Cuts at: 0 1024 1031 1944
Size: 1024 7 913
Fragments arranged by size:
1024 913 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1162 1944
Size: 1162 782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 798 833 1496 1531 1944
Size: 798 35 663 35 413
Fragments arranged by size:
798 663 413 35 35
EaeI y'GGCC_r
Cuts at: 0 1074 1944
Size: 1074 870
EarI CTCTTCn'nnn_
Cuts at: 0 19 338 872 983 1944
Size: 19 319 534 111 961
Fragments arranged by size:
961 534 319 111 19
EcoICRI GAG'CTC
Cuts at: 0 1686 1944
Size: 1686 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 427 475 938 1312 1615 1807 1944
Size: 43 384 48 463 374 303 192 137
Fragments arranged by size:
463 384 374 303 192 137 48 43
EcoO109I rG'GnC_Cy
Cuts at: 0 72 269 504 732 866 1428 1544 1944
Size: 72 197 235 228 134 562 116 400
Fragments arranged by size:
562 400 235 228 197 134 116 72
HaeII r_GCGC'y
Cuts at: 0 1803 1944
Size: 1803 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1456 1488 1729 1761 1924 1944
Size: 1456 32 241 32 163 20
Fragments arranged by size:
1456 241 163 32 32 20
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1944
Size: 997 947
MslI CAynn'nnrTG
Cuts at: 0 34 95 158 1372 1944
Size: 34 61 63 1214 572
Fragments arranged by size:
1214 572 63 61 34
MspA1I CmG'CkG
Cuts at: 0 158 1013 1704 1846 1944
Size: 158 855 691 142 98
Fragments arranged by size:
855 691 158 142 98
NcoI C'CATG_G
Cuts at: 0 1031 1944
Size: 1031 913
NspI r_CATG'y
Cuts at: 0 1404 1516 1608 1944
Size: 1404 112 92 336
Fragments arranged by size:
1404 336 112 92
PasI CC'CwG_GG
Cuts at: 0 326 1944
Size: 326 1618
PciI A'CATG_T
Cuts at: 0 1604 1944
Size: 1604 340
PfoI T'CCnGG_A
Cuts at: 0 1289 1346 1944
Size: 1289 57 598
Fragments arranged by size:
1289 598 57
PpuMI rG'GwC_Cy
Cuts at: 0 269 732 866 1428 1544 1944
Size: 269 463 134 562 116 400
Fragments arranged by size:
562 463 400 269 134 116
PspOMI G'GGCC_C
Cuts at: 0 71 504 1944
Size: 71 433 1440
Fragments arranged by size:
1440 433 71
PstI C_TGCA'G
Cuts at: 0 190 550 1944
Size: 190 360 1394
Fragments arranged by size:
1394 360 190
PvuII CAG'CTG
Cuts at: 0 1704 1846 1944
Size: 1704 142 98
Fragments arranged by size:
1704 142 98
SacI G_AGCT'C
Cuts at: 0 1688 1944
Size: 1688 256
SanDI GG'GwC_CC
Cuts at: 0 1544 1944
Size: 1544 400
SapI GCTCTTCn'nnn_
Cuts at: 0 19 1944
Size: 19 1925
SfcI C'TryA_G
Cuts at: 0 41 186 546 1944
Size: 41 145 360 1398
Fragments arranged by size:
1398 360 145 41
SmaI CCC'GGG
Cuts at: 0 70 1944
Size: 70 1874
SmlI C'TyrA_G
Cuts at: 0 632 1174 1432 1944
Size: 632 542 258 512
Fragments arranged by size:
632 542 512 258
SnaBI TAC'GTA
Cuts at: 0 1557 1944
Size: 1557 387
SrfI GCCC'GGGC
Cuts at: 0 70 1944
Size: 70 1874
StuI AGG'CCT
Cuts at: 0 1141 1944
Size: 1141 803
StyI C'CwwG_G
Cuts at: 0 109 719 1031 1944
Size: 109 610 312 913
Fragments arranged by size:
913 610 312 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 1944
Size: 184 1760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 574 606 1583 1615 1944
Size: 574 32 977 32 329
Fragments arranged by size:
977 574 329 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 657 1944
Size: 657 1287
XmaI C'CCGG_G
Cuts at: 0 68 1944
Size: 68 1876
XmnI GAAnn'nnTTC
Cuts at: 0 746 1944
Size: 746 1198
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApoI AvaI BanI BanII BbvCI BcgI BclI
BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI
CspCI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I
MmeI MslI MspA1I NcoI NspI PasI PciI PfoI
PpuMI PspOMI PstI PvuII SacI SanDI SapI SfcI
SmaI SmlI SnaBI SrfI StuI StyI TaqII TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BciVI BfrBI BlpI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI
DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SpeI SphI SspI SwaI TatI Tth111I XbaI XhoI
ZraI