Restriction map for
pFN21AE0994
(Linear) (Six-base) MAPSORT of: KIEE0994.seq Check: 1354 from: 1 to: 1941
KIEE0994.orf
With 182 enzymes: SgfI *
March 22, 2012 15:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 86 1017 1941
Size: 86 931 924
Fragments arranged by size:
931 924 86
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 946 1285 1888 1941
Size: 266 680 339 603 53
Fragments arranged by size:
680 603 339 266 53
AflIII A'CryG_T
Cuts at: 0 1320 1941
Size: 1320 621
AleI CACnn'nnGTG
Cuts at: 0 139 1941
Size: 139 1802
AlwNI CAG_nnn'CTG
Cuts at: 0 349 840 1941
Size: 349 491 1101
Fragments arranged by size:
1101 491 349
ApaI G_GGCC'C
Cuts at: 0 126 343 964 1941
Size: 126 217 621 977
Fragments arranged by size:
977 621 217 126
ApaLI G'TGCA_C
Cuts at: 0 1030 1941
Size: 1030 911
AvaI C'yCGr_G
Cuts at: 0 562 602 1171 1807 1941
Size: 562 40 569 636 134
Fragments arranged by size:
636 569 562 134 40
BanI G'GyrC_C
Cuts at: 0 615 1787 1941
Size: 615 1172 154
Fragments arranged by size:
1172 615 154
BanII G_rGCy'C
Cuts at: 0 126 343 964 1310 1376 1846 1941
Size: 126 217 621 346 66 470 95
Fragments arranged by size:
621 470 346 217 126 95 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 1673 1941
Size: 1673 268
BbvCI CC'TCA_GC
Cuts at: 0 1192 1783 1941
Size: 1192 591 158
Fragments arranged by size:
1192 591 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 757 791 1941
Size: 757 34 1150
Fragments arranged by size:
1150 757 34
BclI T'GATC_A
Cuts at: 0 107 1941
Size: 107 1834
BglI GCCn_nnn'nGGC
Cuts at: 0 358 547 1941
Size: 358 189 1394
Fragments arranged by size:
1394 358 189
BglII A'GATC_T
Cuts at: 0 884 1397 1941
Size: 884 513 544
Fragments arranged by size:
884 544 513
BlpI GC'TnA_GC
Cuts at: 0 589 841 1375 1941
Size: 589 252 534 566
Fragments arranged by size:
589 566 534 252
Bme1580I G_kGCm'C
Cuts at: 0 126 343 684 964 1034 1245 1941
Size: 126 217 341 280 70 211 696
Fragments arranged by size:
696 341 280 217 211 126 70
BmgBI CAC'GTC
Cuts at: 0 1188 1941
Size: 1188 753
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 741 1941
Size: 741 1200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1231 1941
Size: 1231 710
Bpu10I CC'TnA_GC
Cuts at: 0 551 1192 1783 1941
Size: 551 641 591 158
Fragments arranged by size:
641 591 551 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1941
Size: 808 1133
BsaAI yAC'GTr
Cuts at: 0 357 1941
Size: 357 1584
BsaHI Gr'CG_yC
Cuts at: 0 1670 1941
Size: 1670 271
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 393 1009 1915 1941
Size: 393 616 906 26
Fragments arranged by size:
906 616 393 26
BseYI C'CCAG_C
Cuts at: 0 342 744 828 1493 1934 1941
Size: 342 402 84 665 441 7
Fragments arranged by size:
665 441 402 342 84 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1810 1941
Size: 193 1617 131
Fragments arranged by size:
1617 193 131
BsiEI CG_ry'CG
Cuts at: 0 65 1056 1941
Size: 65 991 885
Fragments arranged by size:
991 885 65
BsiHKAI G_wGCw'C
Cuts at: 0 1034 1178 1310 1747 1941
Size: 1034 144 132 437 194
Fragments arranged by size:
1034 437 194 144 132
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1798 1941
Size: 1798 143
Bsp1286I G_dGCh'C
Cuts at: 0 126 343 684 964 1034 1178 1245 1310
Size: 126 217 341 280 70 144 67 65
Cuts at: 1310 1376 1747 1846 1941
Size: 66 371 99 95
Fragments arranged by size:
371 341 280 217 144 126 99 95
70 67 66 65
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 86 1017 1268 1941
Size: 86 931 251 673
Fragments arranged by size:
931 673 251 86
BsrBI CCG'CTC
Cuts at: 0 292 609 783 894 999 1941
Size: 292 317 174 111 105 942
Fragments arranged by size:
942 317 292 174 111 105
BsrFI r'CCGG_y
Cuts at: 0 635 1642 1941
Size: 635 1007 299
Fragments arranged by size:
1007 635 299
BstAPI GCAn_nnn'nTGC
Cuts at: 0 800 1919 1941
Size: 800 1119 22
Fragments arranged by size:
1119 800 22
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 141 1084 1338 1941
Size: 30 111 943 254 603
Fragments arranged by size:
943 603 254 111 30
BstYI r'GATC_y
Cuts at: 0 884 1073 1397 1546 1757 1941
Size: 884 189 324 149 211 184
Fragments arranged by size:
884 324 211 189 184 149
Bsu36I CC'TnA_GG
Cuts at: 0 726 1426 1941
Size: 726 700 515
Fragments arranged by size:
726 700 515
BtgI C'CryG_G
Cuts at: 0 65 134 464 662 1941
Size: 65 69 330 198 1279
Fragments arranged by size:
1279 330 198 69 65
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 792 827 1941
Size: 792 35 1114
Fragments arranged by size:
1114 792 35
DraIII CAC_nnn'GTG
Cuts at: 0 1506 1941
Size: 1506 435
EaeI y'GGCC_r
Cuts at: 0 62 116 359 393 633 641 659 1053
Size: 62 54 243 34 240 8 18 394
Cuts at: 1053 1064 1127 1941
Size: 11 63 814
Fragments arranged by size:
814 394 243 240 63 62 54 34
18 11 8
EagI C'GGCC_G
Cuts at: 0 62 1053 1941
Size: 62 991 888
Fragments arranged by size:
991 888 62
EarI CTCTTCn'nnn_
Cuts at: 0 285 697 1941
Size: 285 412 1244
Fragments arranged by size:
1244 412 285
EcoICRI GAG'CTC
Cuts at: 0 1308 1941
Size: 1308 633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 946 1231 1285 1888 1941
Size: 266 680 285 54 603 53
Fragments arranged by size:
680 603 285 266 54 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 766 1264 1360 1699 1941
Size: 766 498 96 339 242
Fragments arranged by size:
766 498 339 242 96
EcoO109I rG'GnC_Cy
Cuts at: 0 122 123 339 513 720 961 1257 1941
Size: 122 1 216 174 207 241 296 684
Fragments arranged by size:
684 296 241 216 207 174 122 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1893 1941
Size: 1893 48
FspI TGC'GCA
Cuts at: 0 793 1941
Size: 793 1148
FspAI rTGC'GCAy
Cuts at: 0 793 1941
Size: 793 1148
HaeII r_GCGC'y
Cuts at: 0 310 1941
Size: 310 1631
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 442 474 927 959 1327 1359
Size: 8 32 402 32 453 32 368 32
Cuts at: 1359 1776 1808 1941
Size: 417 32 133
Fragments arranged by size:
453 417 402 368 133 32 32 32
32 32 8
HincII GTy'rAC
Cuts at: 0 1116 1941
Size: 1116 825
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1499 1941
Size: 962 537 442
Fragments arranged by size:
962 537 442
MscI TGG'CCA
Cuts at: 0 361 643 1066 1941
Size: 361 282 423 875
Fragments arranged by size:
875 423 361 282
MslI CAynn'nnrTG
Cuts at: 0 25 28 139 1129 1627 1679 1941
Size: 25 3 111 990 498 52 262
Fragments arranged by size:
990 498 262 111 52 25 3
NaeI GCC'GGC
Cuts at: 0 637 1941
Size: 637 1304
NcoI C'CATG_G
Cuts at: 0 464 1941
Size: 464 1477
NgoMIV G'CCGG_C
Cuts at: 0 635 1941
Size: 635 1306
NotI GC'GGCC_GC
Cuts at: 0 1053 1941
Size: 1053 888
NspI r_CATG'y
Cuts at: 0 159 256 601 1152 1324 1941
Size: 159 97 345 551 172 617
Fragments arranged by size:
617 551 345 172 159 97
PasI CC'CwG_GG
Cuts at: 0 318 1941
Size: 318 1623
PciI A'CATG_T
Cuts at: 0 1320 1941
Size: 1320 621
PfoI T'CCnGG_A
Cuts at: 0 848 1941
Size: 848 1093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1618 1650 1941
Size: 1618 32 291
Fragments arranged by size:
1618 291 32
PpuMI rG'GwC_Cy
Cuts at: 0 720 1257 1941
Size: 720 537 684
Fragments arranged by size:
720 684 537
PspOMI G'GGCC_C
Cuts at: 0 122 339 960 1941
Size: 122 217 621 981
Fragments arranged by size:
981 621 217 122
PstI C_TGCA'G
Cuts at: 0 83 131 1315 1941
Size: 83 48 1184 626
Fragments arranged by size:
1184 626 83 48
SacI G_AGCT'C
Cuts at: 0 1310 1941
Size: 1310 631
SapI GCTCTTCn'nnn_
Cuts at: 0 697 1941
Size: 697 1244
SfcI C'TryA_G
Cuts at: 0 79 127 1311 1908 1941
Size: 79 48 1184 597 33
Fragments arranged by size:
1184 597 79 48 33
SmaI CCC'GGG
Cuts at: 0 604 1941
Size: 604 1337
SmlI C'TyrA_G
Cuts at: 0 823 1941
Size: 823 1118
SphI G_CATG'C
Cuts at: 0 256 601 1941
Size: 256 345 1340
Fragments arranged by size:
1340 345 256
SspI AAT'ATT
Cuts at: 0 199 1941
Size: 199 1742
StuI AGG'CCT
Cuts at: 0 41 400 1163 1705 1906 1941
Size: 41 359 763 542 201 35
Fragments arranged by size:
763 542 359 201 41 35
StyI C'CwwG_G
Cuts at: 0 464 1941
Size: 464 1477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 505 1692 1941
Size: 505 1187 249
Fragments arranged by size:
1187 505 249
XmaI C'CCGG_G
Cuts at: 0 602 1941
Size: 602 1339
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I HincII MmeI
MscI MslI NaeI NcoI NgoMIV NotI NspI PasI
PciI PfoI PpiI PpuMI PspOMI PstI SacI SapI
SfcI SmaI SmlI SphI SspI StuI StyI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BfrBI BmrI BmtI BsaI BsaBI
BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI
DraI DrdI EciI EcoRI EcoRV FseI HindIII HpaI
KasI KpnI MfeI MluI MspA1I NarI NdeI NheI
NruI NsiI PacI PflMI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmnI ZraI