Restriction map for
pFN21AE0982
(Linear) (Six-base) MAPSORT of: KIEE0982.seq Check: 1111 from: 1 to: 1761
KIEE0982.orf
With 182 enzymes: SgfI *
March 22, 2012 15:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1170 1761
Size: 1170 591
Acc65I G'GTAC_C
Cuts at: 0 578 1067 1761
Size: 578 489 694
Fragments arranged by size:
694 578 489
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 857 1660 1761
Size: 469 388 803 101
Fragments arranged by size:
803 469 388 101
AfeI AGC'GCT
Cuts at: 0 1219 1761
Size: 1219 542
AflIII A'CryG_T
Cuts at: 0 632 1052 1619 1761
Size: 632 420 567 142
Fragments arranged by size:
632 567 420 142
AgeI A'CCGG_T
Cuts at: 0 1109 1761
Size: 1109 652
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 286 1761
Size: 286 1475
AlwNI CAG_nnn'CTG
Cuts at: 0 800 1761
Size: 800 961
ApaI G_GGCC'C
Cuts at: 0 696 1761
Size: 696 1065
AscI GG'CGCG_CC
Cuts at: 0 1403 1761
Size: 1403 358
AvaI C'yCGr_G
Cuts at: 0 1540 1657 1708 1761
Size: 1540 117 51 53
Fragments arranged by size:
1540 117 53 51
BanI G'GyrC_C
Cuts at: 0 209 578 1067 1112 1158 1594 1761
Size: 209 369 489 45 46 436 167
Fragments arranged by size:
489 436 369 209 167 46 45
BanII G_rGCy'C
Cuts at: 0 410 696 940 965 1361 1761
Size: 410 286 244 25 396 400
Fragments arranged by size:
410 400 396 286 244 25
BbeI G_GCGC'C
Cuts at: 0 213 1162 1598 1761
Size: 213 949 436 163
Fragments arranged by size:
949 436 213 163
BbsI GAAGACnn'nnnn_
Cuts at: 0 758 1761
Size: 758 1003
BbvCI CC'TCA_GC
Cuts at: 0 1169 1761
Size: 1169 592
BclI T'GATC_A
Cuts at: 0 525 1761
Size: 525 1236
BglI GCCn_nnn'nGGC
Cuts at: 0 1261 1761
Size: 1261 500
Bme1580I G_kGCm'C
Cuts at: 0 265 358 696 952 1761
Size: 265 93 338 256 809
Fragments arranged by size:
809 338 265 256 93
BmgBI CAC'GTC
Cuts at: 0 1176 1620 1761
Size: 1176 444 141
Fragments arranged by size:
1176 444 141
BmrI ACTGGGnnnn_n'
Cuts at: 0 1056 1599 1690 1761
Size: 1056 543 91 71
Fragments arranged by size:
1056 543 91 71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 984 1761
Size: 984 777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 724 745 886 1114 1492 1761
Size: 367 357 21 141 228 378 269
Fragments arranged by size:
378 367 357 269 228 141 21
Bpu10I CC'TnA_GC
Cuts at: 0 157 769 1169 1761
Size: 157 612 400 592
Fragments arranged by size:
612 592 400 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 475 1761
Size: 394 81 1286
Fragments arranged by size:
1286 394 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 319 721 1761
Size: 319 402 1040
Fragments arranged by size:
1040 402 319
BsaAI yAC'GTr
Cuts at: 0 1055 1761
Size: 1055 706
BsaBI GATnn'nnATC
Cuts at: 0 199 1761
Size: 199 1562
BsaHI Gr'CG_yC
Cuts at: 0 210 1121 1159 1595 1761
Size: 210 911 38 436 166
Fragments arranged by size:
911 436 210 166 38
BsaWI w'CCGG_w
Cuts at: 0 1109 1761
Size: 1109 652
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 406 436 581 611 1195 1225 1261 1291
Size: 406 30 145 30 584 30 36 30
Cuts at: 1291 1761
Size: 470
Fragments arranged by size:
584 470 406 145 36 30 30 30
30
BseYI C'CCAG_C
Cuts at: 0 688 1761
Size: 688 1073
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1077 1198 1632 1750 1761
Size: 421 656 121 434 118 11
Fragments arranged by size:
656 434 421 121 118 11
BsiEI CG_ry'CG
Cuts at: 0 110 1400 1572 1761
Size: 110 1290 172 189
Fragments arranged by size:
1290 189 172 110
BsiHKAI G_wGCw'C
Cuts at: 0 251 491 1498 1517 1715 1761
Size: 251 240 1007 19 198 46
Fragments arranged by size:
1007 251 240 198 46 19
BsmI GAATG_Cn'
Cuts at: 0 683 1761
Size: 683 1078
Bsp1286I G_dGCh'C
Cuts at: 0 251 265 358 410 491 696 940 952
Size: 251 14 93 52 81 205 244 12
Cuts at: 952 965 1361 1498 1517 1715 1761
Size: 13 396 137 19 198 46
Fragments arranged by size:
396 251 244 205 198 137 93 81
52 46 19 14 13 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 278 1170 1673 1761
Size: 278 892 503 88
Fragments arranged by size:
892 503 278 88
BsrFI r'CCGG_y
Cuts at: 0 643 1109 1399 1455 1761
Size: 643 466 290 56 306
Fragments arranged by size:
643 466 306 290 56
BssHII G'CGCG_C
Cuts at: 0 1146 1251 1403 1441 1761
Size: 1146 105 152 38 320
Fragments arranged by size:
1146 320 152 105 38
BssSI C'ACGA_G
Cuts at: 0 484 945 1726 1761
Size: 484 461 781 35
Fragments arranged by size:
781 484 461 35
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1425 1761
Size: 1425 336
BstYI r'GATC_y
Cuts at: 0 872 1761
Size: 872 889
Bsu36I CC'TnA_GG
Cuts at: 0 953 1761
Size: 953 808
BtgI C'CryG_G
Cuts at: 0 110 402 890 1244 1412 1418 1761
Size: 110 292 488 354 168 6 343
Fragments arranged by size:
488 354 343 292 168 110 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 6 176 1761
Size: 6 170 1585
Fragments arranged by size:
1585 170 6
BtsI GCAGTG_nn'
Cuts at: 0 1489 1761
Size: 1489 272
DrdI GACnn_nn'nnGTC
Cuts at: 0 229 1761
Size: 229 1532
EaeI y'GGCC_r
Cuts at: 0 107 1019 1397 1415 1457 1569 1761
Size: 107 912 378 18 42 112 192
Fragments arranged by size:
912 378 192 112 107 42 18
EagI C'GGCC_G
Cuts at: 0 107 1397 1569 1761
Size: 107 1290 172 192
Fragments arranged by size:
1290 192 172 107
EarI CTCTTCn'nnn_
Cuts at: 0 362 805 1471 1761
Size: 362 443 666 290
Fragments arranged by size:
666 443 362 290
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 414 1444 1761
Size: 414 1030 317
Fragments arranged by size:
1030 414 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 469 724 745 857 886 1114 1492
Size: 367 102 255 21 112 29 228 378
Cuts at: 1492 1660 1761
Size: 168 101
Fragments arranged by size:
378 367 255 228 168 112 102 101
29 21
EcoO109I rG'GnC_Cy
Cuts at: 0 444 480 692 1662 1761
Size: 444 36 212 970 99
Fragments arranged by size:
970 444 212 99 36
FspI TGC'GCA
Cuts at: 0 823 1761
Size: 823 938
HaeII r_GCGC'y
Cuts at: 0 57 213 1162 1221 1278 1548 1598 1761
Size: 57 156 949 59 57 270 50 163
Fragments arranged by size:
949 270 163 156 59 57 57 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 510 542 1761
Size: 510 32 1219
Fragments arranged by size:
1219 510 32
HincII GTy'rAC
Cuts at: 0 63 1761
Size: 63 1698
KasI G'GCGC_C
Cuts at: 0 209 1158 1594 1761
Size: 209 949 436 167
Fragments arranged by size:
949 436 209 167
KpnI G_GTAC'C
Cuts at: 0 582 1071 1761
Size: 582 489 690
Fragments arranged by size:
690 582 489
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 1067 1761
Size: 93 974 694
Fragments arranged by size:
974 694 93
MslI CAynn'nnrTG
Cuts at: 0 631 945 1761
Size: 631 314 816
Fragments arranged by size:
816 631 314
MspA1I CmG'CkG
Cuts at: 0 396 404 907 1038 1761
Size: 396 8 503 131 723
Fragments arranged by size:
723 503 396 131 8
NaeI GCC'GGC
Cuts at: 0 645 1401 1457 1761
Size: 645 756 56 304
Fragments arranged by size:
756 645 304 56
NarI GG'CG_CC
Cuts at: 0 210 1159 1595 1761
Size: 210 949 436 166
Fragments arranged by size:
949 436 210 166
NcoI C'CATG_G
Cuts at: 0 890 1244 1761
Size: 890 354 517
Fragments arranged by size:
890 517 354
NgoMIV G'CCGG_C
Cuts at: 0 643 1399 1455 1761
Size: 643 756 56 306
Fragments arranged by size:
756 643 306 56
NspI r_CATG'y
Cuts at: 0 376 636 1761
Size: 376 260 1125
Fragments arranged by size:
1125 376 260
PasI CC'CwG_GG
Cuts at: 0 75 1761
Size: 75 1686
PciI A'CATG_T
Cuts at: 0 632 1761
Size: 632 1129
PflMI CCAn_nnn'nTGG
Cuts at: 0 75 702 1761
Size: 75 627 1059
Fragments arranged by size:
1059 627 75
PfoI T'CCnGG_A
Cuts at: 0 311 722 743 1370 1469 1761
Size: 311 411 21 627 99 292
Fragments arranged by size:
627 411 311 292 99 21
PmlI CAC'GTG
Cuts at: 0 1055 1761
Size: 1055 706
PpuMI rG'GwC_Cy
Cuts at: 0 444 480 1662 1761
Size: 444 36 1182 99
Fragments arranged by size:
1182 444 99 36
PspOMI G'GGCC_C
Cuts at: 0 692 1761
Size: 692 1069
PstI C_TGCA'G
Cuts at: 0 275 1761
Size: 275 1486
PvuII CAG'CTG
Cuts at: 0 396 907 1038 1761
Size: 396 511 131 723
Fragments arranged by size:
723 511 396 131
SacII CC_GC'GG
Cuts at: 0 405 1761
Size: 405 1356
SapI GCTCTTCn'nnn_
Cuts at: 0 362 1761
Size: 362 1399
SbfI CC_TGCA'GG
Cuts at: 0 275 1761
Size: 275 1486
SfcI C'TryA_G
Cuts at: 0 271 1761
Size: 271 1490
SfoI GGC'GCC
Cuts at: 0 211 1160 1596 1761
Size: 211 949 436 165
Fragments arranged by size:
949 436 211 165
SmlI C'TyrA_G
Cuts at: 0 409 490 1761
Size: 409 81 1271
Fragments arranged by size:
1271 409 81
SphI G_CATG'C
Cuts at: 0 376 1761
Size: 376 1385
StuI AGG'CCT
Cuts at: 0 816 1741 1761
Size: 816 925 20
Fragments arranged by size:
925 816 20
StyI C'CwwG_G
Cuts at: 0 257 890 1244 1761
Size: 257 633 354 517
Fragments arranged by size:
633 517 354 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 241 343 1750 1761
Size: 241 102 1407 11
Fragments arranged by size:
1407 241 102 11
TatI w'GTAC_w
Cuts at: 0 317 413 1761
Size: 317 96 1348
Fragments arranged by size:
1348 317 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 581 613 1081 1113 1195 1227 1761
Size: 581 32 468 32 82 32 534
Fragments arranged by size:
581 534 468 82 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 861 1368 1467 1761
Size: 861 507 99 294
Fragments arranged by size:
861 507 294 99
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AgeI AlfI AlwNI
ApaI AscI AvaI BanI BanII BbeI BbsI BbvCI
BclI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BssHII
BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I FspI HaeII
Hin4I HincII KasI KpnI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuII SacII SapI SbfI
SfcI SfoI SmlI SphI StuI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AflII AhdI AleI AloI ApaLI
ApoI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BfrBI BglII BlpI BmtI BseRI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstZ17I
ClaI CspCI DraI DraIII EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI MfeI MluI MscI
NdeI NheI NotI NruI NsiI PacI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI