Restriction map for
pFN21AE0980
(Linear) (Six-base) MAPSORT of: KIEE0980.seq Check: 5931 from: 1 to: 1734
KIEE0980.orf
With 182 enzymes: SgfI *
July 3, 2012 16:42 ..
AccI GT'mk_AC
Cuts at: 0 216 1117 1734
Size: 216 901 617
Fragments arranged by size:
901 617 216
Acc65I G'GTAC_C
Cuts at: 0 1602 1734
Size: 1602 132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 847 1734
Size: 271 576 887
Fragments arranged by size:
887 576 271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 52 1734
Size: 52 1682
AlwNI CAG_nnn'CTG
Cuts at: 0 87 777 1471 1734
Size: 87 690 694 263
Fragments arranged by size:
694 690 263 87
ApoI r'AATT_y
Cuts at: 0 128 259 921 1671 1734
Size: 128 131 662 750 63
Fragments arranged by size:
750 662 131 128 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 814 847 1586 1619 1734
Size: 814 33 739 33 115
Fragments arranged by size:
814 739 115 33 33
BanI G'GyrC_C
Cuts at: 0 51 1059 1602 1734
Size: 51 1008 543 132
Fragments arranged by size:
1008 543 132 51
BanII G_rGCy'C
Cuts at: 0 1338 1734
Size: 1338 396
BbsI GAAGACnn'nnnn_
Cuts at: 0 277 1329 1734
Size: 277 1052 405
Fragments arranged by size:
1052 405 277
BbvCI CC'TCA_GC
Cuts at: 0 660 1734
Size: 660 1074
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1588 1622 1734
Size: 1588 34 112
Fragments arranged by size:
1588 112 34
BclI T'GATC_A
Cuts at: 0 305 1710 1734
Size: 305 1405 24
Fragments arranged by size:
1405 305 24
BglI GCCn_nnn'nGGC
Cuts at: 0 73 1734
Size: 73 1661
BlpI GC'TnA_GC
Cuts at: 0 1657 1734
Size: 1657 77
BmgBI CAC'GTC
Cuts at: 0 1493 1734
Size: 1493 241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1734
Size: 769 965
Bpu10I CC'TnA_GC
Cuts at: 0 660 1734
Size: 660 1074
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1734
Size: 937 797
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1435 1465 1734
Size: 1435 30 269
Fragments arranged by size:
1435 269 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1372 1541 1734
Size: 1372 169 193
Fragments arranged by size:
1372 193 169
BseYI C'CCAG_C
Cuts at: 0 536 1610 1734
Size: 536 1074 124
Fragments arranged by size:
1074 536 124
BsiEI CG_ry'CG
Cuts at: 0 1048 1734
Size: 1048 686
BsiHKAI G_wGCw'C
Cuts at: 0 1338 1597 1722 1734
Size: 1338 259 125 12
Fragments arranged by size:
1338 259 125 12
BsmI GAATG_Cn'
Cuts at: 0 1431 1734
Size: 1431 303
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1625 1734
Size: 1625 109
Bsp1286I G_dGCh'C
Cuts at: 0 1338 1597 1722 1734
Size: 1338 259 125 12
Fragments arranged by size:
1338 259 125 12
BspHI T'CATG_A
Cuts at: 0 837 1734
Size: 837 897
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1614 1734
Size: 1614 120
BsrFI r'CCGG_y
Cuts at: 0 25 1734
Size: 25 1709
BstEII G'GTnAC_C
Cuts at: 0 633 1734
Size: 633 1101
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1012 1098 1734
Size: 1012 86 636
Fragments arranged by size:
1012 636 86
BstYI r'GATC_y
Cuts at: 0 357 705 1229 1351 1468 1734
Size: 357 348 524 122 117 266
Fragments arranged by size:
524 357 348 266 122 117
BtgI C'CryG_G
Cuts at: 0 1091 1347 1734
Size: 1091 256 387
Fragments arranged by size:
1091 387 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1205 1487 1734
Size: 1205 282 247
Fragments arranged by size:
1205 282 247
BtsI GCAGTG_nn'
Cuts at: 0 1005 1734
Size: 1005 729
EaeI y'GGCC_r
Cuts at: 0 1014 1103 1734
Size: 1014 89 631
Fragments arranged by size:
1014 631 89
EarI CTCTTCn'nnn_
Cuts at: 0 227 1276 1421 1734
Size: 227 1049 145 313
Fragments arranged by size:
1049 313 227 145
EcoICRI GAG'CTC
Cuts at: 0 1336 1734
Size: 1336 398
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 769 847 1734
Size: 271 498 78 887
Fragments arranged by size:
887 498 271 78
EcoO109I rG'GnC_Cy
Cuts at: 0 774 1649 1734
Size: 774 875 85
Fragments arranged by size:
875 774 85
EcoRI G'AATT_C
Cuts at: 0 259 921 1734
Size: 259 662 813
Fragments arranged by size:
813 662 259
EcoRV GAT'ATC
Cuts at: 0 1532 1734
Size: 1532 202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 492 524 1320 1352 1371 1403 1734
Size: 492 32 796 32 19 32 331
Fragments arranged by size:
796 492 331 32 32 32 19
KpnI G_GTAC'C
Cuts at: 0 1606 1734
Size: 1606 128
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1091 1734
Size: 448 643 643
Fragments arranged by size:
643 643 448
MscI TGG'CCA
Cuts at: 0 1016 1734
Size: 1016 718
MslI CAynn'nnrTG
Cuts at: 0 1096 1734
Size: 1096 638
MspA1I CmG'CkG
Cuts at: 0 57 146 1734
Size: 57 89 1588
Fragments arranged by size:
1588 89 57
NaeI GCC'GGC
Cuts at: 0 27 1734
Size: 27 1707
NcoI C'CATG_G
Cuts at: 0 1091 1347 1734
Size: 1091 256 387
Fragments arranged by size:
1091 387 256
NdeI CA'TA_TG
Cuts at: 0 801 1734
Size: 801 933
NgoMIV G'CCGG_C
Cuts at: 0 25 1734
Size: 25 1709
NspI r_CATG'y
Cuts at: 0 1152 1734
Size: 1152 582
PshAI GACnn'nnGTC
Cuts at: 0 899 1734
Size: 899 835
PstI C_TGCA'G
Cuts at: 0 92 1734
Size: 92 1642
PvuII CAG'CTG
Cuts at: 0 146 1734
Size: 146 1588
SacI G_AGCT'C
Cuts at: 0 1338 1734
Size: 1338 396
SapI GCTCTTCn'nnn_
Cuts at: 0 1421 1734
Size: 1421 313
ScaI AGT'ACT
Cuts at: 0 751 1734
Size: 751 983
SfcI C'TryA_G
Cuts at: 0 88 1734
Size: 88 1646
SmlI C'TyrA_G
Cuts at: 0 916 1734
Size: 916 818
SphI G_CATG'C
Cuts at: 0 1152 1734
Size: 1152 582
SspI AAT'ATT
Cuts at: 0 487 815 1734
Size: 487 328 919
Fragments arranged by size:
919 487 328
StuI AGG'CCT
Cuts at: 0 209 1734
Size: 209 1525
StyI C'CwwG_G
Cuts at: 0 546 1091 1347 1734
Size: 546 545 256 387
Fragments arranged by size:
546 545 387 256
TatI w'GTAC_w
Cuts at: 0 749 1734
Size: 749 985
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 119 151 1734
Size: 119 32 1583
Fragments arranged by size:
1583 119 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1098 1734
Size: 1098 636
XmnI GAAnn'nnTTC
Cuts at: 0 487 1734
Size: 487 1247
Enzymes that do cut:
AccI Acc65I AcuI AlfI AlwNI ApoI BaeI BanI
BanII BbsI BbvCI BcgI BclI BglI BlpI BmgBI
BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BstEII BstXI
BstYI BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV Hin4I KpnI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NspI PshAI PstI
PvuII SacI SapI ScaI SfcI SmlI SphI SspI
StuI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BciVI BfrBI BglII Bme1580I BmrI
BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsgI
BsiWI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI NarI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI
TaqII Tth111I XbaI XhoI XmaI ZraI